Analysis of nuclear transport signals in the human apurinic/apyrimidinic endonuclease (APE1/Ref1)

Elias B. Jackson, Corey A. Theriot, Ranajoy Chattopadhyay, Sankar Mitra, Tadahide Izumi

Research output: Contribution to journalArticle

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Abstract

The mammalian abasic-endonuclease1/redox-factor1 (APE1/Ref1) is an essential protein whose subcellular distribution depends on the cellular physiological status. However, its nuclear localization signals have not been studied in detail. We examined nuclear translocation of APE1, by monitoring enhanced green fluorescent protein (EGFP) fused to APE1. APE1's nuclear localization was significantly decreased by deleting 20 amino acid residues from its N-terminus. Fusion of APE1's N-terminal 20 residues directed nuclear localization of EGFP. An APE1 mutant lacking the seven N-terminal residues (ND7 APE1) showed nearly normal nuclear localization, which was drastically reduced when the deletion was combined with the E12A/D13A double mutation. On the other hand, nearly normal nuclear localization of the full-length E12A/D13A mutant suggests that the first 7 residues and residues 8-13 can independently promote nuclear import. Both far-western analyses and immuno-pull-down assays indicate interaction of APE1 with karyopherin alpha 1 and 2, which requires the 20 N-terminal residues and implicates nuclear importins in APE1's nuclear translocation. Nuclear accumulation of the ND7 APE1(E12A/D13A) mutant after treatment with the nuclear export inhibitor leptomycin B suggests the presence of a previously unidentified nuclear export signal, and the subcellular distribution of APE1 may be regulated by both nuclear import and export.

Original languageEnglish (US)
Pages (from-to)3303-3312
Number of pages10
JournalNucleic Acids Research
Volume33
Issue number10
DOIs
StatePublished - 2005

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DNA-(Apurinic or Apyrimidinic Site) Lyase
Cell Nucleus Active Transport
Nuclear Export Signals
Karyopherins
Nuclear Localization Signals
Oxidation-Reduction
Amino Acids
Mutation
Proteins
enhanced green fluorescent protein

ASJC Scopus subject areas

  • Genetics

Cite this

Jackson, E. B., Theriot, C. A., Chattopadhyay, R., Mitra, S., & Izumi, T. (2005). Analysis of nuclear transport signals in the human apurinic/apyrimidinic endonuclease (APE1/Ref1). Nucleic Acids Research, 33(10), 3303-3312. https://doi.org/10.1093/nar/gki641

Analysis of nuclear transport signals in the human apurinic/apyrimidinic endonuclease (APE1/Ref1). / Jackson, Elias B.; Theriot, Corey A.; Chattopadhyay, Ranajoy; Mitra, Sankar; Izumi, Tadahide.

In: Nucleic Acids Research, Vol. 33, No. 10, 2005, p. 3303-3312.

Research output: Contribution to journalArticle

Jackson, EB, Theriot, CA, Chattopadhyay, R, Mitra, S & Izumi, T 2005, 'Analysis of nuclear transport signals in the human apurinic/apyrimidinic endonuclease (APE1/Ref1)', Nucleic Acids Research, vol. 33, no. 10, pp. 3303-3312. https://doi.org/10.1093/nar/gki641
Jackson, Elias B. ; Theriot, Corey A. ; Chattopadhyay, Ranajoy ; Mitra, Sankar ; Izumi, Tadahide. / Analysis of nuclear transport signals in the human apurinic/apyrimidinic endonuclease (APE1/Ref1). In: Nucleic Acids Research. 2005 ; Vol. 33, No. 10. pp. 3303-3312.
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