Analysis of radiation-induced chromosome exchanges using combinatorial chromosome painting

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Combinatorial chromosome painting techniques such as multiplex fluorescence in situ hybridization (mFISH) or Spectral Karyotyping (SKY) follow basic fluorescence in situ hybridization (FISH) procedures but use combinations of fluorochromes to label probes to specific chromosomes in such a way that each chromosome is painted with a unique signal. Such signals are captured with image analysis systems allowing the construction of karyotypes with each chromosome unambiguously identified. These systems allow chromosomal analysis in great detail and are particularly useful for the detection of complex chromosome exchanges that originate from three or more breaks. This chapter will describe methods that can be used to analyze the results obtained in mFISH karyotypes particularly with relation to complex chromosome exchanges.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages123-135
Number of pages13
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1984
ISSN (Print)1064-3745

Keywords

  • Complex chromosome exchanges
  • Cycle structure
  • Karyotyping
  • mFISH
  • Rejoining cycle
  • SKY

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Loucas, B. (2019). Analysis of radiation-induced chromosome exchanges using combinatorial chromosome painting. In Methods in Molecular Biology (pp. 123-135). (Methods in Molecular Biology; Vol. 1984). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9432-8_15