Analysis of receptor tyrosine kinase internalization using flow cytometry

Ning Li, Kristen S. Hill, Lisa A. Elferink

Research output: Chapter in Book/Report/Conference proceedingChapter

20 Scopus citations

Abstract

The internalization of activated receptor tyrosine kinases (RTKs) by endocytosis and their subsequent down regulation in lysosomes plays a critical role in regulating the duration and intensity of downstream signaling events. Uncoupling of the RTK cMet from ligand-induced degradation was recently shown to correlate with sustained receptor signaling and increased cell tumorigenicity, suggesting that the corruption of these endocytic mechanisms could contribute to increased cMet signaling in metastatic cancers. To understand how cMet signaling for normal cell growth is controlled by endocytosis and how these mechanisms are dysregulated in metastatic cancers, we developed flow cytometry-based assays to examine cMet internalization.

Original languageEnglish (US)
Title of host publicationMembrane Trafficking
PublisherHumana Press Inc.
Pages305-317
Number of pages13
ISBN (Print)9781588299253
DOIs
StatePublished - 2008

Publication series

NameMethods in Molecular Biology
Volume457
ISSN (Print)1064-3745

Keywords

  • CMet
  • Endocytosis
  • Flow cytometry
  • Hepatocyte growth factor
  • Internalin B
  • Receptor tyrosine kinase

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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