Analysis of sputum/tracheal aspirate and nasopharyngeal samples for SARS-CoV-2 detection by laboratory-developed test and Panther Fusion system

Phyu M. Thwe; Ping Ren

doi:10.1016/j.diagmicrobio.2020.115228

Analysis of sputum/tracheal aspirate and nasopharyngeal samples for SARS-CoV-2 detection by laboratory-developed test and Panther Fusion system

Phyu M. Thwe, Ping Ren

Pathology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

In this study, 127 sputum/tracheal aspirate specimens were evaluated by a laboratory-developed real-time RT-PCR method and Fusion SARS-CoV-2 assay. These specimens were collected from the patients who have nasopharyngeal swab (NPS) samples being used for SARS-CoV-2 detection previously or simultaneously. The overall agreement was 96% between the lower respiratory tract (LRT) and NPS samples, suggesting that LRT specimens could be an option for patients who develop a productive cough or those receiving invasive mechanical ventilation.

Original language	English (US)
Article number	115228
Journal	Diagnostic Microbiology and Infectious Disease
Volume	99
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2020.115228
State	Published - Jan 2021

Keywords

COVID-19
Lower respiratory tract
Nasopharyngeal swab sample
SARS-CoV-2
Sputum
Tracheal aspirate

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

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title = "Analysis of sputum/tracheal aspirate and nasopharyngeal samples for SARS-CoV-2 detection by laboratory-developed test and Panther Fusion system",

abstract = "In this study, 127 sputum/tracheal aspirate specimens were evaluated by a laboratory-developed real-time RT-PCR method and Fusion SARS-CoV-2 assay. These specimens were collected from the patients who have nasopharyngeal swab (NPS) samples being used for SARS-CoV-2 detection previously or simultaneously. The overall agreement was 96% between the lower respiratory tract (LRT) and NPS samples, suggesting that LRT specimens could be an option for patients who develop a productive cough or those receiving invasive mechanical ventilation.",

keywords = "COVID-19, Lower respiratory tract, Nasopharyngeal swab sample, SARS-CoV-2, Sputum, Tracheal aspirate",

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AB - In this study, 127 sputum/tracheal aspirate specimens were evaluated by a laboratory-developed real-time RT-PCR method and Fusion SARS-CoV-2 assay. These specimens were collected from the patients who have nasopharyngeal swab (NPS) samples being used for SARS-CoV-2 detection previously or simultaneously. The overall agreement was 96% between the lower respiratory tract (LRT) and NPS samples, suggesting that LRT specimens could be an option for patients who develop a productive cough or those receiving invasive mechanical ventilation.

KW - COVID-19

KW - Lower respiratory tract

KW - Nasopharyngeal swab sample

KW - SARS-CoV-2

KW - Sputum

KW - Tracheal aspirate

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Analysis of sputum/tracheal aspirate and nasopharyngeal samples for SARS-CoV-2 detection by laboratory-developed test and Panther Fusion system

Abstract

Keywords

ASJC Scopus subject areas

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