Analysis of the local organization and dynamics of cellular actin networks

Weiwei Luo, Cheng han Yu, Zi Zhao Lieu, Jun Allard, Alex Mogilner, Michael Sheetz, Alexander D. Bershadsky

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

A ctin filaments, with the aid of multiple accessory proteins, self-assemble into a variety of network patterns. We studied the organization and dynamics of the actin network in nonadhesive regions of cells bridging fibronectin-coated adhesive strips. The network was formed by actin nodes associated with and linked by myosin II and containing the formin disheveled-associated activator of morphogenesis 1 (DAAM1) and the crosslinker filamin A (FlnA). After Latrunculin A (LatA) addition, actin nodes appeared to be more prominent and demonstrated drift-diffusion motion. Superresolution microscopy revealed that, in untreated cells, DAAM1 formed patches with a similar spatial arrangement to the actin nodes. Node movement (diffusion coefficient and velocity) in LatA-treated cells was dependent on the level and activity of myosin IIA, DAAM1, and FlnA. Based on our results, we developed a computational model of the dynamic formin-filamin-actin asters that can self-organize into a contractile actomyosin network. We suggest that such networks are critical for connecting distant parts of the cell to maintain the mechanical coherence of the cytoplasm.

Original languageEnglish (US)
Pages (from-to)1057-1073
Number of pages17
JournalJournal of Cell Biology
Volume202
Issue number7
DOIs
StatePublished - Oct 25 2013
Externally publishedYes

Fingerprint

Filamins
Actins
Morphogenesis
Nonmuscle Myosin Type IIA
Myosin Type II
Actomyosin
Fibronectins
Adhesives
Microscopy
Cytoplasm
Proteins
latrunculin A

ASJC Scopus subject areas

  • Cell Biology

Cite this

Luo, W., Yu, C. H., Lieu, Z. Z., Allard, J., Mogilner, A., Sheetz, M., & Bershadsky, A. D. (2013). Analysis of the local organization and dynamics of cellular actin networks. Journal of Cell Biology, 202(7), 1057-1073. https://doi.org/10.1083/jcb.201210123

Analysis of the local organization and dynamics of cellular actin networks. / Luo, Weiwei; Yu, Cheng han; Lieu, Zi Zhao; Allard, Jun; Mogilner, Alex; Sheetz, Michael; Bershadsky, Alexander D.

In: Journal of Cell Biology, Vol. 202, No. 7, 25.10.2013, p. 1057-1073.

Research output: Contribution to journalArticle

Luo, W, Yu, CH, Lieu, ZZ, Allard, J, Mogilner, A, Sheetz, M & Bershadsky, AD 2013, 'Analysis of the local organization and dynamics of cellular actin networks', Journal of Cell Biology, vol. 202, no. 7, pp. 1057-1073. https://doi.org/10.1083/jcb.201210123
Luo, Weiwei ; Yu, Cheng han ; Lieu, Zi Zhao ; Allard, Jun ; Mogilner, Alex ; Sheetz, Michael ; Bershadsky, Alexander D. / Analysis of the local organization and dynamics of cellular actin networks. In: Journal of Cell Biology. 2013 ; Vol. 202, No. 7. pp. 1057-1073.
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