Analysis of the pentafluorobenzoyl derivative of phenylethylamine utilizing negative ion chemical ionization and gas chromatography/mass spectrometry.

P. Gashkoff, Reuben Matalon, V. Papa, D. Sebasta

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A negative ion chemical ionization (NICI) mass spectrometric procedure has been developed to measure phenylethylamine in human urine as the pentafluorobenzoyl derivative. The aromatic amine was isolated from alkaline urine by hexane and ethyl acetate extraction followed by a back-extraction into diluted acid. The acid extract was lyophilized and the residue derivatized with (2,3,4,5,6)-pentafluorobenzoyl chloride. Quantification was achieved by comparing the peak areas of the [M-HF]- ion to a ratio of internal and external standards. The phenylethylamine levels excreted by normal subjects and patients with phenylketonuria were found to be consistent with previous reports.

Original languageEnglish (US)
Pages (from-to)327-329
Number of pages3
JournalBiomedical & environmental mass spectrometry
Volume16
Issue number1-12
StatePublished - Oct 1988
Externally publishedYes

Fingerprint

Phenethylamines
Gas chromatography
Gas Chromatography-Mass Spectrometry
Ionization
Mass spectrometry
Negative ions
Urine
Ions
Derivatives
Phenylketonurias
Acids
Hexanes
Hexane
Amines
pentafluorobenzoyl chloride
ethyl acetate

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Pollution

Cite this

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abstract = "A negative ion chemical ionization (NICI) mass spectrometric procedure has been developed to measure phenylethylamine in human urine as the pentafluorobenzoyl derivative. The aromatic amine was isolated from alkaline urine by hexane and ethyl acetate extraction followed by a back-extraction into diluted acid. The acid extract was lyophilized and the residue derivatized with (2,3,4,5,6)-pentafluorobenzoyl chloride. Quantification was achieved by comparing the peak areas of the [M-HF]- ion to a ratio of internal and external standards. The phenylethylamine levels excreted by normal subjects and patients with phenylketonuria were found to be consistent with previous reports.",
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T1 - Analysis of the pentafluorobenzoyl derivative of phenylethylamine utilizing negative ion chemical ionization and gas chromatography/mass spectrometry.

AU - Gashkoff, P.

AU - Matalon, Reuben

AU - Papa, V.

AU - Sebasta, D.

PY - 1988/10

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AB - A negative ion chemical ionization (NICI) mass spectrometric procedure has been developed to measure phenylethylamine in human urine as the pentafluorobenzoyl derivative. The aromatic amine was isolated from alkaline urine by hexane and ethyl acetate extraction followed by a back-extraction into diluted acid. The acid extract was lyophilized and the residue derivatized with (2,3,4,5,6)-pentafluorobenzoyl chloride. Quantification was achieved by comparing the peak areas of the [M-HF]- ion to a ratio of internal and external standards. The phenylethylamine levels excreted by normal subjects and patients with phenylketonuria were found to be consistent with previous reports.

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