Angiopoietin-1 inhibits endothelial cell apoptosis via the Akt/survivin pathway

Andreas Papapetropoulos, David Fulton, Keyvan Mahboubi, Robert G. Kalb, Daniel S. O'Connor, Fengzhi Li, Dario C. Altieri, William C. Sessa

Research output: Contribution to journalArticle

505 Citations (Scopus)

Abstract

A productive angiogenic response must couple to the survival machinery of endothelial cells to preserve the integrity of newly formed vessels. Angiopoietin-1 (Ang-1) is an endothelium-specific ligand essential for embryonic vascular stabilization, branching morphogenesis, and post-natal angiogenesis, but its contribution to endothelial cell survival has not been completely elucidated. Here we show that Ang-1 acting via the Tie 2 receptor induces phosphorylation of the survival serine-threonine kinase, Akt (or protein kinase B). This is associated with up-regulation of the apoptosis inhibitor, survivin, in endothelial cells and protection of endothelium from death-inducing stimuli. Moreover, dominant negative survivin negates the ability of Ang-1 to protect cells from undergoing apoptosis. The activation of anti-apoptotic pathways mediated by Akt and survivin in endothelial cells may contribute to Ang-1 stabilization of vascular structures during angiogenesis, in vivo.

Original languageEnglish (US)
Pages (from-to)9102-9105
Number of pages4
JournalJournal of Biological Chemistry
Volume275
Issue number13
DOIs
StatePublished - Mar 31 2000
Externally publishedYes

Fingerprint

Angiopoietin-1
Endothelial cells
Endothelial Cells
Apoptosis
Endothelium
Blood Vessels
Stabilization
Proto-Oncogene Proteins c-akt
Phosphorylation
Cytoprotection
Protein-Serine-Threonine Kinases
Morphogenesis
Machinery
Cell Survival
Up-Regulation
Chemical activation
Ligands

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Papapetropoulos, A., Fulton, D., Mahboubi, K., Kalb, R. G., O'Connor, D. S., Li, F., ... Sessa, W. C. (2000). Angiopoietin-1 inhibits endothelial cell apoptosis via the Akt/survivin pathway. Journal of Biological Chemistry, 275(13), 9102-9105. https://doi.org/10.1074/jbc.275.13.9102

Angiopoietin-1 inhibits endothelial cell apoptosis via the Akt/survivin pathway. / Papapetropoulos, Andreas; Fulton, David; Mahboubi, Keyvan; Kalb, Robert G.; O'Connor, Daniel S.; Li, Fengzhi; Altieri, Dario C.; Sessa, William C.

In: Journal of Biological Chemistry, Vol. 275, No. 13, 31.03.2000, p. 9102-9105.

Research output: Contribution to journalArticle

Papapetropoulos, A, Fulton, D, Mahboubi, K, Kalb, RG, O'Connor, DS, Li, F, Altieri, DC & Sessa, WC 2000, 'Angiopoietin-1 inhibits endothelial cell apoptosis via the Akt/survivin pathway', Journal of Biological Chemistry, vol. 275, no. 13, pp. 9102-9105. https://doi.org/10.1074/jbc.275.13.9102
Papapetropoulos, Andreas ; Fulton, David ; Mahboubi, Keyvan ; Kalb, Robert G. ; O'Connor, Daniel S. ; Li, Fengzhi ; Altieri, Dario C. ; Sessa, William C. / Angiopoietin-1 inhibits endothelial cell apoptosis via the Akt/survivin pathway. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 13. pp. 9102-9105.
@article{b30a920379b34c26a46484943898f4da,
title = "Angiopoietin-1 inhibits endothelial cell apoptosis via the Akt/survivin pathway",
abstract = "A productive angiogenic response must couple to the survival machinery of endothelial cells to preserve the integrity of newly formed vessels. Angiopoietin-1 (Ang-1) is an endothelium-specific ligand essential for embryonic vascular stabilization, branching morphogenesis, and post-natal angiogenesis, but its contribution to endothelial cell survival has not been completely elucidated. Here we show that Ang-1 acting via the Tie 2 receptor induces phosphorylation of the survival serine-threonine kinase, Akt (or protein kinase B). This is associated with up-regulation of the apoptosis inhibitor, survivin, in endothelial cells and protection of endothelium from death-inducing stimuli. Moreover, dominant negative survivin negates the ability of Ang-1 to protect cells from undergoing apoptosis. The activation of anti-apoptotic pathways mediated by Akt and survivin in endothelial cells may contribute to Ang-1 stabilization of vascular structures during angiogenesis, in vivo.",
author = "Andreas Papapetropoulos and David Fulton and Keyvan Mahboubi and Kalb, {Robert G.} and O'Connor, {Daniel S.} and Fengzhi Li and Altieri, {Dario C.} and Sessa, {William C.}",
year = "2000",
month = "3",
day = "31",
doi = "10.1074/jbc.275.13.9102",
language = "English (US)",
volume = "275",
pages = "9102--9105",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "13",

}

TY - JOUR

T1 - Angiopoietin-1 inhibits endothelial cell apoptosis via the Akt/survivin pathway

AU - Papapetropoulos, Andreas

AU - Fulton, David

AU - Mahboubi, Keyvan

AU - Kalb, Robert G.

AU - O'Connor, Daniel S.

AU - Li, Fengzhi

AU - Altieri, Dario C.

AU - Sessa, William C.

PY - 2000/3/31

Y1 - 2000/3/31

N2 - A productive angiogenic response must couple to the survival machinery of endothelial cells to preserve the integrity of newly formed vessels. Angiopoietin-1 (Ang-1) is an endothelium-specific ligand essential for embryonic vascular stabilization, branching morphogenesis, and post-natal angiogenesis, but its contribution to endothelial cell survival has not been completely elucidated. Here we show that Ang-1 acting via the Tie 2 receptor induces phosphorylation of the survival serine-threonine kinase, Akt (or protein kinase B). This is associated with up-regulation of the apoptosis inhibitor, survivin, in endothelial cells and protection of endothelium from death-inducing stimuli. Moreover, dominant negative survivin negates the ability of Ang-1 to protect cells from undergoing apoptosis. The activation of anti-apoptotic pathways mediated by Akt and survivin in endothelial cells may contribute to Ang-1 stabilization of vascular structures during angiogenesis, in vivo.

AB - A productive angiogenic response must couple to the survival machinery of endothelial cells to preserve the integrity of newly formed vessels. Angiopoietin-1 (Ang-1) is an endothelium-specific ligand essential for embryonic vascular stabilization, branching morphogenesis, and post-natal angiogenesis, but its contribution to endothelial cell survival has not been completely elucidated. Here we show that Ang-1 acting via the Tie 2 receptor induces phosphorylation of the survival serine-threonine kinase, Akt (or protein kinase B). This is associated with up-regulation of the apoptosis inhibitor, survivin, in endothelial cells and protection of endothelium from death-inducing stimuli. Moreover, dominant negative survivin negates the ability of Ang-1 to protect cells from undergoing apoptosis. The activation of anti-apoptotic pathways mediated by Akt and survivin in endothelial cells may contribute to Ang-1 stabilization of vascular structures during angiogenesis, in vivo.

UR - http://www.scopus.com/inward/record.url?scp=0034737422&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034737422&partnerID=8YFLogxK

U2 - 10.1074/jbc.275.13.9102

DO - 10.1074/jbc.275.13.9102

M3 - Article

VL - 275

SP - 9102

EP - 9105

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 13

ER -