Angiotensin II induces gene transcription through cell-type-dependent effects on the nuclear factor-κB (NF-κB) transcription factor

A. R. Brasier, M. Jamaluddin, Y. Han, C. Patterson, M. S. Runge

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

The vasopressor octapeptide, angiotensin II (Ang II), exerts homeostatic responses in cardiovascular tissues, including the heart, blood vessel wall, adrenal cortex and liver (a major source of circulating plasma proteins). One of the effects of Ang II is to induce expression of regulatory, structural and cytokine genes that play important roles in long-term control of blood pressure, vascular remodeling, cardiac hypertrophy and inflammation. The identification of nuclear signaling pathways and target transcription factors has provide important insight into cellular responses and the spectrum of genes controlled by Ang II. Here we will review how Ang II activates the transcription factors, Activator Protein 1 (AP-1), Signal Transducer and Activator of Transcription (STATs), and Nuclear Factor-κB (NF-κB). NF-κB is of particular interest because it is an important mediator of resynthesis of the Ang II precursor, angiotensinogen AGT. Through this positive feedback loop, long-term changes in the activity of the renin angiotensin system occur. Although NF-κB is ubiquitously expressed, surprisingly the mechanism for Ang II-inducible NF-κB regulation differs between aortic smooth muscle cells (VSMCs) and hepatocytes. In VSMC, Ang II induces nuclear translocation of cytoplasmic transactivatory NF-κB proteins through proteolysis of its inhibitor, IκB. By contrast, in hepatocytes, Ang II induces large nuclear isoforms of NF-κB1 to bind DNA through a mechanism independent of changes in IκB turnover. NF-κB activation depends upon the activity of DAG-sensitive PKC isoforms and ROS signaling pathway. These observations indicate that significant differences exist in Ang II signaling depending upon cell-type involved and suggest the possibility that tissue-selective modulation of Ang II effects is possible in the cardiovascular system.

Original languageEnglish (US)
Pages (from-to)155-169
Number of pages15
JournalMolecular and Cellular Biochemistry
Volume212
Issue number1-2
StatePublished - 2000

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Transcription
Angiotensin II
Transcription Factors
Genes
Hepatocytes
Protein Isoforms
3'-(1-butylphosphoryl)adenosine
Tissue
Proteolysis
Cardiovascular system
Angiotensinogen
Adrenal Cortex
Blood pressure
Transcription Factor AP-1
Angiotensins
Blood vessels
Cardiomegaly
Renin-Angiotensin System
Cardiovascular System
Transducers

Keywords

  • Angiotensin II
  • AP-1
  • Hepatocyte
  • Myocyte
  • NF-κB
  • NF-κB1
  • Renin Angiotensin System (RAS)
  • STAT
  • Transcription factor

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Angiotensin II induces gene transcription through cell-type-dependent effects on the nuclear factor-κB (NF-κB) transcription factor. / Brasier, A. R.; Jamaluddin, M.; Han, Y.; Patterson, C.; Runge, M. S.

In: Molecular and Cellular Biochemistry, Vol. 212, No. 1-2, 2000, p. 155-169.

Research output: Contribution to journalArticle

Brasier, A. R. ; Jamaluddin, M. ; Han, Y. ; Patterson, C. ; Runge, M. S. / Angiotensin II induces gene transcription through cell-type-dependent effects on the nuclear factor-κB (NF-κB) transcription factor. In: Molecular and Cellular Biochemistry. 2000 ; Vol. 212, No. 1-2. pp. 155-169.
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