Angiotensin II induces IL-6 expression and the Jak-STAT3 pathway in aortic adventitia of LDL receptor-deficient mice

Adrian Recinos, Wanda S. LeJeune, Hong Sun, Chang Y. Lee, Brian C. Tieu, Muping Lu, Tieying Hou, Istvan Boldogh, Ronald Tilton, Allan R. Brasier

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Angiotensin II (A-II), the major effector peptide of the renin angiotensin system potently accelerates progression of atherosclerosis. To investigate its effects on vascular inflammatory mechanisms, we elucidated vascular cytokine expression during early lesion development in A-II-infused atherosclerosis-prone LDLR-/- mice. Male LDLR-/- mice were placed on a "Western" high-fat diet for 4 weeks, followed by sham or A-II infusion for 7 weeks. Equal blood pressures and elevations in serum lipids were seen in both groups. Mice were sacrificed when significant A-II-induced plaque development was first detectable, aortae were explanted and culture media assayed for secreted cytokines. Nine cytokines were significantly induced with interleukin-6 (IL-6) being the most highly secreted. Local IL-6 production was confirmed by in situ mRNA hybridization and immunostaining, where the most abundant IL-6 was found in the aortic adventitia, with lesser production by the medial and intimal layers. Immunofluorescence colocalization showed IL-6 expression by fibroblasts and activated macrophages. Activation of downstream IL-6 signaling mediated by the Jak-STAT3 pathway was demonstrated by inducible phospho-Tyr705-STAT3 formation in the adventitia and endothelium (of IL-6+/+mice only). These findings define cytokine profiles in the A-II infusion model and demonstrate that IL-6, produced by activated macrophages and fibroblasts in the adventitia, induces the Jak-STAT3 pathway during early A-II-induced atherosclerosis.

Original languageEnglish (US)
Pages (from-to)125-133
Number of pages9
JournalAtherosclerosis
Volume194
Issue number1
DOIs
StatePublished - Sep 2007

Fingerprint

Adventitia
LDL Receptors
Angiotensin II
Interleukin-6
Cytokines
Atherosclerosis
Blood Vessels
Fibroblasts
Macrophages
Tunica Intima
High Fat Diet
Renin-Angiotensin System
Endothelium
In Situ Hybridization
Fluorescent Antibody Technique
Culture Media
Aorta
Blood Pressure
Lipids
Messenger RNA

Keywords

  • Angiotensin II
  • Atherosclerosis
  • Cytokine
  • Inflammation
  • Interleukin-6
  • LDL receptor knock-out mouse
  • STAT3

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Angiotensin II induces IL-6 expression and the Jak-STAT3 pathway in aortic adventitia of LDL receptor-deficient mice. / Recinos, Adrian; LeJeune, Wanda S.; Sun, Hong; Lee, Chang Y.; Tieu, Brian C.; Lu, Muping; Hou, Tieying; Boldogh, Istvan; Tilton, Ronald; Brasier, Allan R.

In: Atherosclerosis, Vol. 194, No. 1, 09.2007, p. 125-133.

Research output: Contribution to journalArticle

Recinos, A, LeJeune, WS, Sun, H, Lee, CY, Tieu, BC, Lu, M, Hou, T, Boldogh, I, Tilton, R & Brasier, AR 2007, 'Angiotensin II induces IL-6 expression and the Jak-STAT3 pathway in aortic adventitia of LDL receptor-deficient mice', Atherosclerosis, vol. 194, no. 1, pp. 125-133. https://doi.org/10.1016/j.atherosclerosis.2006.10.013
Recinos, Adrian ; LeJeune, Wanda S. ; Sun, Hong ; Lee, Chang Y. ; Tieu, Brian C. ; Lu, Muping ; Hou, Tieying ; Boldogh, Istvan ; Tilton, Ronald ; Brasier, Allan R. / Angiotensin II induces IL-6 expression and the Jak-STAT3 pathway in aortic adventitia of LDL receptor-deficient mice. In: Atherosclerosis. 2007 ; Vol. 194, No. 1. pp. 125-133.
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AU - Tieu, Brian C.

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AB - Angiotensin II (A-II), the major effector peptide of the renin angiotensin system potently accelerates progression of atherosclerosis. To investigate its effects on vascular inflammatory mechanisms, we elucidated vascular cytokine expression during early lesion development in A-II-infused atherosclerosis-prone LDLR-/- mice. Male LDLR-/- mice were placed on a "Western" high-fat diet for 4 weeks, followed by sham or A-II infusion for 7 weeks. Equal blood pressures and elevations in serum lipids were seen in both groups. Mice were sacrificed when significant A-II-induced plaque development was first detectable, aortae were explanted and culture media assayed for secreted cytokines. Nine cytokines were significantly induced with interleukin-6 (IL-6) being the most highly secreted. Local IL-6 production was confirmed by in situ mRNA hybridization and immunostaining, where the most abundant IL-6 was found in the aortic adventitia, with lesser production by the medial and intimal layers. Immunofluorescence colocalization showed IL-6 expression by fibroblasts and activated macrophages. Activation of downstream IL-6 signaling mediated by the Jak-STAT3 pathway was demonstrated by inducible phospho-Tyr705-STAT3 formation in the adventitia and endothelium (of IL-6+/+mice only). These findings define cytokine profiles in the A-II infusion model and demonstrate that IL-6, produced by activated macrophages and fibroblasts in the adventitia, induces the Jak-STAT3 pathway during early A-II-induced atherosclerosis.

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