Angiotensin II induces nuclear factor (NF)-κB1 isoforms to bind the angiotensinogen gene acute-phase response element

A stimulus-specific pathway for NF-κB activation

Mohammad Jamaluddin, Tao Meng, Juan Sun, Istvan Boldogh, Youqi Han, Allan R. Brasier

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The vasopressor angiotensin II (AII) activates transcriptional expression of its precursor, angiotensinogen. This biological "positive feedback loop" occurs through an angiotensin receptor-coupled pathway that activates a multihormone-responsive enhancer of the angiotensinogen promoter, termed the acute-phase response element (APRE). Previously, we showed that the APRE is a cytokine [tumor necrosis factor-α (TNFα)]- inducible enhancer by binding the heterodimeric nuclear factor-κB (NF-κB) complex Rel A•NF-κB1. Here, we compare the mechanism for NF-κB activation by the All agonist, Sar1 All, with TNFα in HepG2 hepatocytes. Although Sar1 All and TNFα both rapidly activate APRE-driven transcription within 3 h of treatment, the pattern of inducible NF-κB binding activity in electrophoretic mobility shift assay is distinct. In contrast to the TNFα mechanism, which strongly induces Rel A•NF-κB1 binding, Sar1 All selectively activates a heterogenous pattern of NF-κB1 binding. Using a two-step microaffinity DNA binding assay, we observe that Sar1 All recruits 50-, 56-, and 96-kDa NF-κB1 isoforms to bind the APRE. Binding of all three NF-κB1 isoforms occurs independently of changes in their nuclear abundance or proteolysis of cytoplasmic IκB inhibitors. Phorbol ester-sensitive protein kinase C (PKC) isoforms are required because PKC down-regulation completely blocks All-inducible transcription and inducible NF-κB1 binding. We conclude that All stimulates the NF-κB transcription factor pathway by activating latent DNA-binding activity of NF-κB subunits through a phorbol ester-sensitive (PKC-dependent) mechanism.

Original languageEnglish (US)
Pages (from-to)99-113
Number of pages15
JournalMolecular Endocrinology
Volume14
Issue number1
DOIs
StatePublished - 2000

Fingerprint

Angiotensinogen
Acute-Phase Reaction
Response Elements
Angiotensin II
Protein Isoforms
Tumor Necrosis Factor-alpha
Protein Kinase C
Atrial Natriuretic Factor
Phorbol Esters
Genes
Angiotensin Receptors
DNA
Electrophoretic Mobility Shift Assay
Proteolysis
Hepatocytes
Transcription Factors
Down-Regulation
Cytokines

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Angiotensin II induces nuclear factor (NF)-κB1 isoforms to bind the angiotensinogen gene acute-phase response element : A stimulus-specific pathway for NF-κB activation. / Jamaluddin, Mohammad; Meng, Tao; Sun, Juan; Boldogh, Istvan; Han, Youqi; Brasier, Allan R.

In: Molecular Endocrinology, Vol. 14, No. 1, 2000, p. 99-113.

Research output: Contribution to journalArticle

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