Animal model of fatal human monocytotropic ehrlichiosis

E. A. Sotomayor, Vsevolod Popov, H. M. Feng, David Walker, Juan Olano

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Human monocytotropic ehrlichiosis caused by Ehrlichia chaffeensis is a life-threatening, tick-borne, emerging infectious disease for which no satisfactory animal model has been developed. Strain HF565, an ehrlichial organism closely related to E. chaffeensis isolated from Ixodes ovatus ticks in Japan, causes fatal infection of mice. C57BL/6 mice became ill on day 7 after inoculation and died on day 9. The liver revealed confluent necrosis, ballooning cell injury, apoptosis, poorly formed granulomas, Kupffer cell hyperplasia, erythrophagocytosis, and microvesicular fatty metamorphosis. The other significant histological findings consisted of marked expansion of the marginal zone and infiltration of the red pulp of the spleen by macrophages, interstitial pneumonitis, and increased numbers of immature myeloid cells and areas of necrosis in the bone marrow. Ehrlichiae were detected by immunohistology and electron microscopy in the liver, lungs, and spleen. The main target cells were macrophages, including Kupffer cells, hepatocytes, and endothelial cells. Apoptosis was detected in Kupffer cells, hepatocytes, and macrophages in the lungs and spleen. This tropism for macrophages and the pathological lesions closely resemble those of human monocytotropic ehrlichiosis for which it is a promising model for investigation of immunity and pathogenesis.

Original languageEnglish (US)
Pages (from-to)757-769
Number of pages13
JournalAmerican Journal of Pathology
Volume158
Issue number2
StatePublished - 2001
Externally publishedYes

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Ehrlichiosis
Kupffer Cells
Ehrlichia chaffeensis
Animal Models
Macrophages
Spleen
Ticks
Hepatocytes
Necrosis
Ehrlichia
Emerging Communicable Diseases
Apoptosis
Ixodes
Lung
Tropism
Liver
Interstitial Lung Diseases
Myeloid Cells
Granuloma
Inbred C57BL Mouse

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Animal model of fatal human monocytotropic ehrlichiosis. / Sotomayor, E. A.; Popov, Vsevolod; Feng, H. M.; Walker, David; Olano, Juan.

In: American Journal of Pathology, Vol. 158, No. 2, 2001, p. 757-769.

Research output: Contribution to journalArticle

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