Animal models for the analysis of immune responses to leishmaniasis.

D. L. Sacks, Peter Melby

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

This unit focuses on the murine model of cutaneous leishmaniasis and models of visceral leishmaniasis in mice and hamsters. Each basic protocol describes the methods used to inoculate parasites and to evaluate infections with regard to lesion progression and visceralization, and quantification of parasite load. Five support protocols are provided; two for the growth and isolation of metacyclic promastigotes from in vitro culture, one for isolation of tissue amastigotes from infected animals, one for cryopreservation of parasites, and one for the preparation of blood agar plates for quantitation of parasite numbers in infected tissue.

Original languageEnglish (US)
JournalCurrent protocols in immunology / edited by John E. Coligan ... [et al.]
VolumeChapter 19
StatePublished - May 2001
Externally publishedYes

Fingerprint

Leishmaniasis
Parasites
Animal Models
Parasite Load
Cutaneous Leishmaniasis
Visceral Leishmaniasis
Cryopreservation
Cricetinae
Agar
Growth
Infection

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{72cd0c2672b64c25bef2cd102f2ec5b0,
title = "Animal models for the analysis of immune responses to leishmaniasis.",
abstract = "This unit focuses on the murine model of cutaneous leishmaniasis and models of visceral leishmaniasis in mice and hamsters. Each basic protocol describes the methods used to inoculate parasites and to evaluate infections with regard to lesion progression and visceralization, and quantification of parasite load. Five support protocols are provided; two for the growth and isolation of metacyclic promastigotes from in vitro culture, one for isolation of tissue amastigotes from infected animals, one for cryopreservation of parasites, and one for the preparation of blood agar plates for quantitation of parasite numbers in infected tissue.",
author = "Sacks, {D. L.} and Peter Melby",
year = "2001",
month = "5",
language = "English (US)",
volume = "Chapter 19",
journal = "Current Protocols in Immunology",
issn = "1934-3671",
publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Animal models for the analysis of immune responses to leishmaniasis.

AU - Sacks, D. L.

AU - Melby, Peter

PY - 2001/5

Y1 - 2001/5

N2 - This unit focuses on the murine model of cutaneous leishmaniasis and models of visceral leishmaniasis in mice and hamsters. Each basic protocol describes the methods used to inoculate parasites and to evaluate infections with regard to lesion progression and visceralization, and quantification of parasite load. Five support protocols are provided; two for the growth and isolation of metacyclic promastigotes from in vitro culture, one for isolation of tissue amastigotes from infected animals, one for cryopreservation of parasites, and one for the preparation of blood agar plates for quantitation of parasite numbers in infected tissue.

AB - This unit focuses on the murine model of cutaneous leishmaniasis and models of visceral leishmaniasis in mice and hamsters. Each basic protocol describes the methods used to inoculate parasites and to evaluate infections with regard to lesion progression and visceralization, and quantification of parasite load. Five support protocols are provided; two for the growth and isolation of metacyclic promastigotes from in vitro culture, one for isolation of tissue amastigotes from infected animals, one for cryopreservation of parasites, and one for the preparation of blood agar plates for quantitation of parasite numbers in infected tissue.

UR - http://www.scopus.com/inward/record.url?scp=45849113668&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=45849113668&partnerID=8YFLogxK

M3 - Article

VL - Chapter 19

JO - Current Protocols in Immunology

JF - Current Protocols in Immunology

SN - 1934-3671

ER -