Anionic-zwitterionic mixed micelles in the micellar electrokinetic separation of clinically relevant steroids on a fused-silica capillary

G. A. Valbuena, L. V. Rao, J. R. Petersen, Anthony Okorodudu, M. G. Bissell, Amin A. Mohammad

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The structural similarities of different clinically relevant steroids make their routine determination in biological samples an analytical challenge. Determination of steroidal hormones in urine and serum is of great interest in clinical endocrinology. A large number of disorders is associated with hypo- or hypersecretion of one or more steroids, for example, Addison's disease, Cushing's syndrome, hirsutism and virilism, adrenal adenomas, hypertension, congenital adrenal hyperplasia etc. A SDS/SB3-12 mixed micellar system was used to achieve baseline separation of eight steroids in less than 10 min. Five different zwitterionic surfactants were evaluated at different concentrations, pH and temperature conditions. The SB3-12/SDS mixed micellar system gave highest resolution and precision. No significant capillary wall interaction or coating effects were observed with the zwitterionic surfactants as is evident from the reproducible migration times (relative standard deviation ≤2%). A detection limit of 1-5 μg/ml was obtained for pure standards.

Original languageEnglish
Pages (from-to)467-474
Number of pages8
JournalJournal of Chromatography A
Volume781
Issue number1-2
DOIs
StatePublished - Sep 26 1997

Fingerprint

Micelles
Fused silica
Silicon Dioxide
Steroids
Surface-Active Agents
Endocrinology
Virilism
Addison Disease
Congenital Adrenal Hyperplasia
Hirsutism
Cushing Syndrome
Adenoma
Limit of Detection
Urine
Hormones
Hypertension
Coatings
Temperature
Serum

Keywords

  • Buffer composition
  • Hormones
  • Steroids

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Anionic-zwitterionic mixed micelles in the micellar electrokinetic separation of clinically relevant steroids on a fused-silica capillary. / Valbuena, G. A.; Rao, L. V.; Petersen, J. R.; Okorodudu, Anthony; Bissell, M. G.; Mohammad, Amin A.

In: Journal of Chromatography A, Vol. 781, No. 1-2, 26.09.1997, p. 467-474.

Research output: Contribution to journalArticle

Valbuena, G. A. ; Rao, L. V. ; Petersen, J. R. ; Okorodudu, Anthony ; Bissell, M. G. ; Mohammad, Amin A. / Anionic-zwitterionic mixed micelles in the micellar electrokinetic separation of clinically relevant steroids on a fused-silica capillary. In: Journal of Chromatography A. 1997 ; Vol. 781, No. 1-2. pp. 467-474.
@article{6fb1ca0add0542b7b6ddca07efd815be,
title = "Anionic-zwitterionic mixed micelles in the micellar electrokinetic separation of clinically relevant steroids on a fused-silica capillary",
abstract = "The structural similarities of different clinically relevant steroids make their routine determination in biological samples an analytical challenge. Determination of steroidal hormones in urine and serum is of great interest in clinical endocrinology. A large number of disorders is associated with hypo- or hypersecretion of one or more steroids, for example, Addison's disease, Cushing's syndrome, hirsutism and virilism, adrenal adenomas, hypertension, congenital adrenal hyperplasia etc. A SDS/SB3-12 mixed micellar system was used to achieve baseline separation of eight steroids in less than 10 min. Five different zwitterionic surfactants were evaluated at different concentrations, pH and temperature conditions. The SB3-12/SDS mixed micellar system gave highest resolution and precision. No significant capillary wall interaction or coating effects were observed with the zwitterionic surfactants as is evident from the reproducible migration times (relative standard deviation ≤2{\%}). A detection limit of 1-5 μg/ml was obtained for pure standards.",
keywords = "Buffer composition, Hormones, Steroids",
author = "Valbuena, {G. A.} and Rao, {L. V.} and Petersen, {J. R.} and Anthony Okorodudu and Bissell, {M. G.} and Mohammad, {Amin A.}",
year = "1997",
month = "9",
day = "26",
doi = "10.1016/S0021-9673(97)00669-9",
language = "English",
volume = "781",
pages = "467--474",
journal = "Journal of Chromatography",
issn = "0021-9673",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Anionic-zwitterionic mixed micelles in the micellar electrokinetic separation of clinically relevant steroids on a fused-silica capillary

AU - Valbuena, G. A.

AU - Rao, L. V.

AU - Petersen, J. R.

AU - Okorodudu, Anthony

AU - Bissell, M. G.

AU - Mohammad, Amin A.

PY - 1997/9/26

Y1 - 1997/9/26

N2 - The structural similarities of different clinically relevant steroids make their routine determination in biological samples an analytical challenge. Determination of steroidal hormones in urine and serum is of great interest in clinical endocrinology. A large number of disorders is associated with hypo- or hypersecretion of one or more steroids, for example, Addison's disease, Cushing's syndrome, hirsutism and virilism, adrenal adenomas, hypertension, congenital adrenal hyperplasia etc. A SDS/SB3-12 mixed micellar system was used to achieve baseline separation of eight steroids in less than 10 min. Five different zwitterionic surfactants were evaluated at different concentrations, pH and temperature conditions. The SB3-12/SDS mixed micellar system gave highest resolution and precision. No significant capillary wall interaction or coating effects were observed with the zwitterionic surfactants as is evident from the reproducible migration times (relative standard deviation ≤2%). A detection limit of 1-5 μg/ml was obtained for pure standards.

AB - The structural similarities of different clinically relevant steroids make their routine determination in biological samples an analytical challenge. Determination of steroidal hormones in urine and serum is of great interest in clinical endocrinology. A large number of disorders is associated with hypo- or hypersecretion of one or more steroids, for example, Addison's disease, Cushing's syndrome, hirsutism and virilism, adrenal adenomas, hypertension, congenital adrenal hyperplasia etc. A SDS/SB3-12 mixed micellar system was used to achieve baseline separation of eight steroids in less than 10 min. Five different zwitterionic surfactants were evaluated at different concentrations, pH and temperature conditions. The SB3-12/SDS mixed micellar system gave highest resolution and precision. No significant capillary wall interaction or coating effects were observed with the zwitterionic surfactants as is evident from the reproducible migration times (relative standard deviation ≤2%). A detection limit of 1-5 μg/ml was obtained for pure standards.

KW - Buffer composition

KW - Hormones

KW - Steroids

UR - http://www.scopus.com/inward/record.url?scp=0030726663&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030726663&partnerID=8YFLogxK

U2 - 10.1016/S0021-9673(97)00669-9

DO - 10.1016/S0021-9673(97)00669-9

M3 - Article

VL - 781

SP - 467

EP - 474

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0021-9673

IS - 1-2

ER -