Ankrd7, a novel gene specifically expressed in Sertoli cells and its potential roles in Sertoli cell maturation

Yu Qiang Shi, Lian Cai Du, Qing Zhong Wang, Chun Fang Han

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The somatic Sertoli cells play an essential role in testis determination and spermatogenesis by providing nutrition and structural support. In the current study, we report on the novel Ankrd7 gene that contains five ankyrin repeat domains. This gene was specifically expressed in Sertoli cells and was regulated in a maturation-dependent manner. Its expression was restricted to testicular tissue, and its mRNA could be detected in testes at as early as 14 dpp (days post partum) using RT-PCR analysis. In both testicular tissue sections and in vitro cultured Sertoli cells, the Ankrd7 protein was localized to the nucleus of the Sertoli cell. Immuno-histochemistry and immunocytochemistry investigations showed that the protein was detectable in testicular tissues at 20 dpp, at which time Sertoli cells were gradually differentiating into their mature cellular form. These results suggest that Ankrd7 is probably involved in the process of Sertoli cell maturation and in spermatogenesis.

Original languageEnglish (US)
Pages (from-to)199-203
Number of pages5
JournalMolecules and Cells
Volume27
Issue number2
DOIs
StatePublished - Feb 2009
Externally publishedYes

Fingerprint

Sertoli Cells
Genes
Spermatogenesis
Testis
Ankyrin Repeat
Cultured Cells
Proteins
Immunohistochemistry
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Ankrd7
  • Ankyrin repeat motif
  • Maturation
  • Sertoli cells

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Ankrd7, a novel gene specifically expressed in Sertoli cells and its potential roles in Sertoli cell maturation. / Shi, Yu Qiang; Du, Lian Cai; Wang, Qing Zhong; Han, Chun Fang.

In: Molecules and Cells, Vol. 27, No. 2, 02.2009, p. 199-203.

Research output: Contribution to journalArticle

Shi, Yu Qiang ; Du, Lian Cai ; Wang, Qing Zhong ; Han, Chun Fang. / Ankrd7, a novel gene specifically expressed in Sertoli cells and its potential roles in Sertoli cell maturation. In: Molecules and Cells. 2009 ; Vol. 27, No. 2. pp. 199-203.
@article{9254f05387fc4e858eb1fe536fbf8fe2,
title = "Ankrd7, a novel gene specifically expressed in Sertoli cells and its potential roles in Sertoli cell maturation",
abstract = "The somatic Sertoli cells play an essential role in testis determination and spermatogenesis by providing nutrition and structural support. In the current study, we report on the novel Ankrd7 gene that contains five ankyrin repeat domains. This gene was specifically expressed in Sertoli cells and was regulated in a maturation-dependent manner. Its expression was restricted to testicular tissue, and its mRNA could be detected in testes at as early as 14 dpp (days post partum) using RT-PCR analysis. In both testicular tissue sections and in vitro cultured Sertoli cells, the Ankrd7 protein was localized to the nucleus of the Sertoli cell. Immuno-histochemistry and immunocytochemistry investigations showed that the protein was detectable in testicular tissues at 20 dpp, at which time Sertoli cells were gradually differentiating into their mature cellular form. These results suggest that Ankrd7 is probably involved in the process of Sertoli cell maturation and in spermatogenesis.",
keywords = "Ankrd7, Ankyrin repeat motif, Maturation, Sertoli cells",
author = "Shi, {Yu Qiang} and Du, {Lian Cai} and Wang, {Qing Zhong} and Han, {Chun Fang}",
year = "2009",
month = "2",
doi = "10.1007/s10059-009-0024-0",
language = "English (US)",
volume = "27",
pages = "199--203",
journal = "Molecules and Cells",
issn = "1016-8478",
publisher = "Korean Society for Molecular and Cellular Biology",
number = "2",

}

TY - JOUR

T1 - Ankrd7, a novel gene specifically expressed in Sertoli cells and its potential roles in Sertoli cell maturation

AU - Shi, Yu Qiang

AU - Du, Lian Cai

AU - Wang, Qing Zhong

AU - Han, Chun Fang

PY - 2009/2

Y1 - 2009/2

N2 - The somatic Sertoli cells play an essential role in testis determination and spermatogenesis by providing nutrition and structural support. In the current study, we report on the novel Ankrd7 gene that contains five ankyrin repeat domains. This gene was specifically expressed in Sertoli cells and was regulated in a maturation-dependent manner. Its expression was restricted to testicular tissue, and its mRNA could be detected in testes at as early as 14 dpp (days post partum) using RT-PCR analysis. In both testicular tissue sections and in vitro cultured Sertoli cells, the Ankrd7 protein was localized to the nucleus of the Sertoli cell. Immuno-histochemistry and immunocytochemistry investigations showed that the protein was detectable in testicular tissues at 20 dpp, at which time Sertoli cells were gradually differentiating into their mature cellular form. These results suggest that Ankrd7 is probably involved in the process of Sertoli cell maturation and in spermatogenesis.

AB - The somatic Sertoli cells play an essential role in testis determination and spermatogenesis by providing nutrition and structural support. In the current study, we report on the novel Ankrd7 gene that contains five ankyrin repeat domains. This gene was specifically expressed in Sertoli cells and was regulated in a maturation-dependent manner. Its expression was restricted to testicular tissue, and its mRNA could be detected in testes at as early as 14 dpp (days post partum) using RT-PCR analysis. In both testicular tissue sections and in vitro cultured Sertoli cells, the Ankrd7 protein was localized to the nucleus of the Sertoli cell. Immuno-histochemistry and immunocytochemistry investigations showed that the protein was detectable in testicular tissues at 20 dpp, at which time Sertoli cells were gradually differentiating into their mature cellular form. These results suggest that Ankrd7 is probably involved in the process of Sertoli cell maturation and in spermatogenesis.

KW - Ankrd7

KW - Ankyrin repeat motif

KW - Maturation

KW - Sertoli cells

UR - http://www.scopus.com/inward/record.url?scp=66149118651&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=66149118651&partnerID=8YFLogxK

U2 - 10.1007/s10059-009-0024-0

DO - 10.1007/s10059-009-0024-0

M3 - Article

VL - 27

SP - 199

EP - 203

JO - Molecules and Cells

JF - Molecules and Cells

SN - 1016-8478

IS - 2

ER -