Anopheles gambiae densovirus (AgDNV) has negligible effects on adult survival and transcriptome of its mosquito host

Xiaoxia Ren, Grant L. Hughes, Guodong Niu, Yasutsugu Suzuki, Jason L. Rasgon

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Mosquito densoviruses (DNVs) are candidate agents for paratransgenic control of malaria and other vector-borne diseases. Unlike other mosquito DNVs, the Anopheles gambiae DNV (AgDNV) is non-pathogenic to larval mosquitoes. However, the cost of infection upon adults and the molecular mechanisms underpinning infection in the mosquito host are unknown. Using life table analysis, we show that AgDNV infection has minimal effects on An. gambiae survival (no significant effect in 2 replicates and a slight 2 day survival decrease in the third replicate). Using microarrays, we show that AgDNV has very minimal effect on the adult mosquito transcriptome, with only 4-15 genes differentially regulated depending on the statistical criteria imposed. The minimal impact upon global transcription provides some mechanistic understanding of lack of virus pathogenicity, suggesting a long co-evolutionary history that has shifted towards avirulence. From an applied standpoint, lack of strong induced fitness costs makes AgDNV an attractive agent for paratransgenic malaria control.

Original languageEnglish (US)
Article numbere584
JournalPeerJ
Volume2014
Issue number1
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Densovirus
Anopheles gambiae
Culicidae
Transcriptome
transcriptome
Malaria control
Transcription
Microarrays
Viruses
Costs
Genes
malaria
Malaria
Infection
infection
Disease Vectors
Costs and Cost Analysis
vector-borne diseases
Life Tables
life tables

Keywords

  • Anopheles
  • Densovirus
  • Fitness
  • Malaria
  • Mosquito
  • Paratransgenesis
  • Transcriptome

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)
  • Neuroscience(all)

Cite this

Anopheles gambiae densovirus (AgDNV) has negligible effects on adult survival and transcriptome of its mosquito host. / Ren, Xiaoxia; Hughes, Grant L.; Niu, Guodong; Suzuki, Yasutsugu; Rasgon, Jason L.

In: PeerJ, Vol. 2014, No. 1, e584, 2014.

Research output: Contribution to journalArticle

Ren, Xiaoxia ; Hughes, Grant L. ; Niu, Guodong ; Suzuki, Yasutsugu ; Rasgon, Jason L. / Anopheles gambiae densovirus (AgDNV) has negligible effects on adult survival and transcriptome of its mosquito host. In: PeerJ. 2014 ; Vol. 2014, No. 1.
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