Anti-G-CSF treatment induces protective tumor immunity in mouse colon cancer by promoting NK cell, macrophage and T cell responses

Katherine T. Morris, Eliseo F. Castillo, Anita L. Ray, Lea L. Weston, Robert A. Nofchissey, Joshua A. Hanson, Von G. Samedi, Irina V. Pinchuk, Laurie G. Hudson, Ellen J. Beswick

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Granulocyte colony-stimulating factor (G-CSF) is a cytokine that is highly expressed in human and mouse colorectal cancers (CRC). We previously reported that G-CSF stimulated human CRC cell growth and migration, therefore in this study we sought to examine the therapeutic potential of anti-G-CSF treatment for CRC. G-CSF is known to mobilize neutrophils, however its impact on other immune cells has not been well examined. Here, we investigated the effects of therapeutic anti-G-CSF treatment on CRC growth and anti-tumor immune responses. C57BL/6 mice treated with azoxymethane/dextran sodium sulfate (AOM/DSS) to induce neoplasms were administered anti-G-CSF or isotype control antibodies three times a week for three weeks. Animals treated with anti-G-CSF antibodies had a marked decrease in neoplasm number and size compared to the isotype control group. Colon neutrophil and macrophage frequency were unchanged, but the number of macrophages producing IL-10 were decreased while IL-12 producing macrophages were increased. NK cells were substantially increased in colons of anti-G-CSF treated mice, along with IFNγ producing CD4+ and CD8+ T cells. These studies are the first to indicate a crucial role for G-CSF inhibition in promoting protective anti-tumor immunity, and suggest that anti-G-CSF treatment is a potential therapeutic approach for CRC.

Original languageEnglish (US)
Pages (from-to)22338-22347
Number of pages10
JournalOncotarget
Volume6
Issue number26
DOIs
StatePublished - 2015

Keywords

  • Colorectal cancer
  • G-CSF
  • Macrophages
  • NK cells
  • Th1

ASJC Scopus subject areas

  • Oncology

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