Anti-inflammatory protein TSG-6 secreted by activated MSCs attenuates zymosan-induced mouse peritonitis by decreasing TLR2/NF-κB signaling in resident macrophages

Hosoon Choi, Ryang Hwa Lee, Nikolay Bazhanov, Joo Youn Oh, Darwin J. Prockop

Research output: Contribution to journalArticle

332 Citations (Scopus)

Abstract

Human mesenchymal stem/progenitor cells (hMSCs) repair tissues and modulate immune systems but the mechanisms are not fully understood. We demonstrated that hMSCs are activated by inflammatory signals to secrete the anti-inflammatory protein, TNF-α-stimulated gene 6 protein (TSG-6) and thereby create a negative feedback loop that reduces inflammation in zymosan-induced peritonitis. The results demonstrate for the first time that TSG-6 interacts through the CD44 receptor on resident macrophages to decrease zymosan/TLR2-mediated nuclear translocation of the NF-κB. The negative feedback loop created by MSCs through TSG-6 attenuates the inflammatory cascade that is initiated by resident macrophages and then amplified by mesothelial cells and probably other cells of the peritoneum. Because inflammation underlies many pathologic processes, including immune responses, the results may explain the beneficial effects of MSCs and TSG-6 in several disease models.

Original languageEnglish (US)
Pages (from-to)330-338
Number of pages9
JournalBlood
Volume118
Issue number2
DOIs
StatePublished - Jul 14 2011
Externally publishedYes

Fingerprint

Zymosan
Macrophages
Peritonitis
Mesenchymal Stromal Cells
Anti-Inflammatory Agents
Genes
Proteins
Stem cells
Inflammation
Feedback
Immune system
Peritoneum
Pathologic Processes
Immune System
Repair
Tissue

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Anti-inflammatory protein TSG-6 secreted by activated MSCs attenuates zymosan-induced mouse peritonitis by decreasing TLR2/NF-κB signaling in resident macrophages. / Choi, Hosoon; Lee, Ryang Hwa; Bazhanov, Nikolay; Oh, Joo Youn; Prockop, Darwin J.

In: Blood, Vol. 118, No. 2, 14.07.2011, p. 330-338.

Research output: Contribution to journalArticle

Choi, Hosoon ; Lee, Ryang Hwa ; Bazhanov, Nikolay ; Oh, Joo Youn ; Prockop, Darwin J. / Anti-inflammatory protein TSG-6 secreted by activated MSCs attenuates zymosan-induced mouse peritonitis by decreasing TLR2/NF-κB signaling in resident macrophages. In: Blood. 2011 ; Vol. 118, No. 2. pp. 330-338.
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