Anti-interleukin 8 autoantibody

interleukin 8 immune complexes visualized by laser confocal microscopy in injured lung: Colocalization with FcγRIIa in lung tissue from acute respiratory distress syndrome patients

Timothy Craig Allen, Rafal Fudala, Sandra E. Nash, Anna Kurdowska

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Context. - Anti-interleukin 8 autoantibody:interleukin 8 (anti-IL-8 autoantibody:IL-8) complexes are present in lung fluids of patients with acute lung injury/acute respiratory distress syndrome (ALI/ARDS), and levels of these complexes correlate with progression to and the outcome of ARDS. FcγRIIa, an immunoglobulin G (IgG) receptor, mediates proinflammatory activity of the complexes. Objective. - To evaluate lung tissues from patients with ARDS for presence of anti-IL-8 autoantibody:IL-8 complexes and to establish whether the complexes associate with FcγRIIa. Design. - Lung tissue sections from 3 patients with ARDS and sections of normal lung tissues from 3 patients were stained with antibodies against IL-8 and IgG to detect immune complexes and with antibody against FcγRIIa. In some experiments, sections were blocked with anti-FcγRIIa antibody before staining. Samples were analyzed using confocal microscopy. Results. - Interleukin 8 costained with IgG and FcγRIIa in lung tissues from patients with ARDS but not in control tissues, suggesting that anti-IL-8 autoantibody:IL-8 complexes are deposited in lungs of patients with ARDS via FcγRIIa. Further, colocalization between IL-8 and FcγRIIa could be blocked by anti-FcγRIIa. Conclusions. - Our data demonstrate that anti-IL-8 autoantibody:IL-8 complexes are present in lung tissues of patients with ARDS, and are attached to FcγRIIa.

Original languageEnglish (US)
Pages (from-to)452-456
Number of pages5
JournalArchives of Pathology and Laboratory Medicine
Volume131
Issue number3
StatePublished - Mar 2007
Externally publishedYes

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Adult Respiratory Distress Syndrome
Antigen-Antibody Complex
Interleukin-8
Confocal Microscopy
Autoantibodies
Lung
Immunoglobulin G
IgG Receptors
Antibodies
Acute Lung Injury
Anti-Idiotypic Antibodies

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

@article{aac44eb235334605a4ed73b31424a942,
title = "Anti-interleukin 8 autoantibody: interleukin 8 immune complexes visualized by laser confocal microscopy in injured lung: Colocalization with FcγRIIa in lung tissue from acute respiratory distress syndrome patients",
abstract = "Context. - Anti-interleukin 8 autoantibody:interleukin 8 (anti-IL-8 autoantibody:IL-8) complexes are present in lung fluids of patients with acute lung injury/acute respiratory distress syndrome (ALI/ARDS), and levels of these complexes correlate with progression to and the outcome of ARDS. FcγRIIa, an immunoglobulin G (IgG) receptor, mediates proinflammatory activity of the complexes. Objective. - To evaluate lung tissues from patients with ARDS for presence of anti-IL-8 autoantibody:IL-8 complexes and to establish whether the complexes associate with FcγRIIa. Design. - Lung tissue sections from 3 patients with ARDS and sections of normal lung tissues from 3 patients were stained with antibodies against IL-8 and IgG to detect immune complexes and with antibody against FcγRIIa. In some experiments, sections were blocked with anti-FcγRIIa antibody before staining. Samples were analyzed using confocal microscopy. Results. - Interleukin 8 costained with IgG and FcγRIIa in lung tissues from patients with ARDS but not in control tissues, suggesting that anti-IL-8 autoantibody:IL-8 complexes are deposited in lungs of patients with ARDS via FcγRIIa. Further, colocalization between IL-8 and FcγRIIa could be blocked by anti-FcγRIIa. Conclusions. - Our data demonstrate that anti-IL-8 autoantibody:IL-8 complexes are present in lung tissues of patients with ARDS, and are attached to FcγRIIa.",
author = "Allen, {Timothy Craig} and Rafal Fudala and Nash, {Sandra E.} and Anna Kurdowska",
year = "2007",
month = "3",
language = "English (US)",
volume = "131",
pages = "452--456",
journal = "Archives of Pathology and Laboratory Medicine",
issn = "0003-9985",
publisher = "College of American Pathologists",
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TY - JOUR

T1 - Anti-interleukin 8 autoantibody

T2 - interleukin 8 immune complexes visualized by laser confocal microscopy in injured lung: Colocalization with FcγRIIa in lung tissue from acute respiratory distress syndrome patients

AU - Allen, Timothy Craig

AU - Fudala, Rafal

AU - Nash, Sandra E.

AU - Kurdowska, Anna

PY - 2007/3

Y1 - 2007/3

N2 - Context. - Anti-interleukin 8 autoantibody:interleukin 8 (anti-IL-8 autoantibody:IL-8) complexes are present in lung fluids of patients with acute lung injury/acute respiratory distress syndrome (ALI/ARDS), and levels of these complexes correlate with progression to and the outcome of ARDS. FcγRIIa, an immunoglobulin G (IgG) receptor, mediates proinflammatory activity of the complexes. Objective. - To evaluate lung tissues from patients with ARDS for presence of anti-IL-8 autoantibody:IL-8 complexes and to establish whether the complexes associate with FcγRIIa. Design. - Lung tissue sections from 3 patients with ARDS and sections of normal lung tissues from 3 patients were stained with antibodies against IL-8 and IgG to detect immune complexes and with antibody against FcγRIIa. In some experiments, sections were blocked with anti-FcγRIIa antibody before staining. Samples were analyzed using confocal microscopy. Results. - Interleukin 8 costained with IgG and FcγRIIa in lung tissues from patients with ARDS but not in control tissues, suggesting that anti-IL-8 autoantibody:IL-8 complexes are deposited in lungs of patients with ARDS via FcγRIIa. Further, colocalization between IL-8 and FcγRIIa could be blocked by anti-FcγRIIa. Conclusions. - Our data demonstrate that anti-IL-8 autoantibody:IL-8 complexes are present in lung tissues of patients with ARDS, and are attached to FcγRIIa.

AB - Context. - Anti-interleukin 8 autoantibody:interleukin 8 (anti-IL-8 autoantibody:IL-8) complexes are present in lung fluids of patients with acute lung injury/acute respiratory distress syndrome (ALI/ARDS), and levels of these complexes correlate with progression to and the outcome of ARDS. FcγRIIa, an immunoglobulin G (IgG) receptor, mediates proinflammatory activity of the complexes. Objective. - To evaluate lung tissues from patients with ARDS for presence of anti-IL-8 autoantibody:IL-8 complexes and to establish whether the complexes associate with FcγRIIa. Design. - Lung tissue sections from 3 patients with ARDS and sections of normal lung tissues from 3 patients were stained with antibodies against IL-8 and IgG to detect immune complexes and with antibody against FcγRIIa. In some experiments, sections were blocked with anti-FcγRIIa antibody before staining. Samples were analyzed using confocal microscopy. Results. - Interleukin 8 costained with IgG and FcγRIIa in lung tissues from patients with ARDS but not in control tissues, suggesting that anti-IL-8 autoantibody:IL-8 complexes are deposited in lungs of patients with ARDS via FcγRIIa. Further, colocalization between IL-8 and FcγRIIa could be blocked by anti-FcγRIIa. Conclusions. - Our data demonstrate that anti-IL-8 autoantibody:IL-8 complexes are present in lung tissues of patients with ARDS, and are attached to FcγRIIa.

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