Anti-Proliferative Naphthalene Glucoside from Aerial Part of Neanotis wightiana

A new bioactive naphthalene glucoside, named neanoside C (1), was isolated from the aerial part of Neanotis wightiana. The structure of neanoside C (1) was elucidated as 1,4-dihydroxy-2-(6′-Omethylglucopyranosyloxymethyl)-naphthalene-3-vinyloxyglucopyranoside by interpretation of the spectroscopic data, including 2D NMR and chemical studies. Neanoside C (1) was further subjected to screening for anticancer potential using MTT based cytotoxicity assay, LDH leakage assay, and estimation of intracellular ROS production in two human breast cancer cell line. The compound exhibited promising anti-proliferative and cytotoxic effect against MCF-7 and MDA-MB-231 cell lines with an IC₅₀ value of 22.77 nM and 35.71 nM, respectively. Neanoside C (1) also exhibited concentration-dependent intracellular LDH leakage and ROS production.