Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ)

Marlyn P. Langford; Patrick W. Gray; G. John Stanton; Bala Lakhchaura; Teh sheng Chan; Howard M. Johnson

doi:10.1016/0006-291X(83)91676-5

Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ)

Marlyn P. Langford, Patrick W. Gray, G. John Stanton, Bala Lakhchaura, Teh sheng Chan, Howard M. Johnson

Microbiology And Immunology

Research output: Contribution to journal › Article

6 Scopus citations

Abstract

Antibodies to an N-terminal synthetic peptide of mouse γ interferon (MoIFNγ) neutralized the antiviral activity of MoIFNγ but not MoIFNα β, human IFNα (HuIFNα), HuIFNβ or cynomologus monkey IFNγ (CynIFNγ). Comparatively, antibodies to mouse N-terminal synthetic peptide showed only 10% reciprocal cross-reactivity in neutralization tests against heterologous HuIFNγ and 13% cross-reactivity in the ELISA test against Hu N-terminal peptide. The predetermined specificity of these antibodies make them powerful tools for studying antigenic relatedness and biological properties of IFNγs.

Original language	English (US)
Pages (from-to)	866-871
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	117
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(83)91676-5
State	Published - Dec 28 1983

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Langford, M. P., Gray, P. W., Stanton, G. J., Lakhchaura, B., Chan, T. S., & Johnson, H. M. (1983). Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ). Biochemical and Biophysical Research Communications, 117(3), 866-871. https://doi.org/10.1016/0006-291X(83)91676-5

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abstract = "Antibodies to an N-terminal synthetic peptide of mouse γ interferon (MoIFNγ) neutralized the antiviral activity of MoIFNγ but not MoIFNα β, human IFNα (HuIFNα), HuIFNβ or cynomologus monkey IFNγ (CynIFNγ). Comparatively, antibodies to mouse N-terminal synthetic peptide showed only 10% reciprocal cross-reactivity in neutralization tests against heterologous HuIFNγ and 13% cross-reactivity in the ELISA test against Hu N-terminal peptide. The predetermined specificity of these antibodies make them powerful tools for studying antigenic relatedness and biological properties of IFNγs.",

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AU - Gray, Patrick W.

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AU - Chan, Teh sheng

AU - Johnson, Howard M.

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