Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ)

Marlyn P. Langford, Patrick W. Gray, G. John Stanton, Bala Lakhchaura, Teh sheng Chan, Howard M. Johnson

Research output: Contribution to journal › Article

6 Citations (Scopus)

Abstract

Antibodies to an N-terminal synthetic peptide of mouse γ interferon (MoIFNγ) neutralized the antiviral activity of MoIFNγ but not MoIFNα β, human IFNα (HuIFNα), HuIFNβ or cynomologus monkey IFNγ (CynIFNγ). Comparatively, antibodies to mouse N-terminal synthetic peptide showed only 10% reciprocal cross-reactivity in neutralization tests against heterologous HuIFNγ and 13% cross-reactivity in the ELISA test against Hu N-terminal peptide. The predetermined specificity of these antibodies make them powerful tools for studying antigenic relatedness and biological properties of IFNγs.

Original languageEnglish (US)
Pages (from-to)866-871
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume117
Issue number3
DOIs
StatePublished - Dec 28 1983

Fingerprint

Interferons
Interferon-gamma
Peptides
Antibodies
Neutralization Tests
Antibody Specificity
Haplorhini
Antiviral Agents
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ). / Langford, Marlyn P.; Gray, Patrick W.; Stanton, G. John; Lakhchaura, Bala; Chan, Teh sheng; Johnson, Howard M.

In: Biochemical and Biophysical Research Communications, Vol. 117, No. 3, 28.12.1983, p. 866-871.

Research output: Contribution to journal › Article

Langford, MP, Gray, PW, Stanton, GJ, Lakhchaura, B, Chan, TS & Johnson, HM 1983, 'Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ)', Biochemical and Biophysical Research Communications, vol. 117, no. 3, pp. 866-871. https://doi.org/10.1016/0006-291X(83)91676-5
Langford MP, Gray PW, Stanton GJ, Lakhchaura B, Chan TS, Johnson HM. Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ). Biochemical and Biophysical Research Communications. 1983 Dec 28;117(3):866-871. https://doi.org/10.1016/0006-291X(83)91676-5
Langford, Marlyn P. ; Gray, Patrick W. ; Stanton, G. John ; Lakhchaura, Bala ; Chan, Teh sheng ; Johnson, Howard M. / Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ). In: Biochemical and Biophysical Research Communications. 1983 ; Vol. 117, No. 3. pp. 866-871.
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