Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ)

Marlyn P. Langford; Patrick W. Gray; G. John Stanton; Bala Lakhchaura; Teh sheng Chan; Howard M. Johnson

doi:10.1016/0006-291X(83)91676-5

Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ)

Marlyn P. Langford, Patrick W. Gray, G. John Stanton, Bala Lakhchaura, Teh sheng Chan, Howard M. Johnson

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Antibodies to an N-terminal synthetic peptide of mouse γ interferon (MoIFNγ) neutralized the antiviral activity of MoIFNγ but not MoIFNα β, human IFNα (HuIFNα), HuIFNβ or cynomologus monkey IFNγ (CynIFNγ). Comparatively, antibodies to mouse N-terminal synthetic peptide showed only 10% reciprocal cross-reactivity in neutralization tests against heterologous HuIFNγ and 13% cross-reactivity in the ELISA test against Hu N-terminal peptide. The predetermined specificity of these antibodies make them powerful tools for studying antigenic relatedness and biological properties of IFNγs.

Original language	English (US)
Pages (from-to)	866-871
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	117
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(83)91676-5
State	Published - Dec 28 1983
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0006-291X(83)91676-5

Cite this

@article{09fb6400f9b740b2aaf26d9c990ea79a,

title = "Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ)",

abstract = "Antibodies to an N-terminal synthetic peptide of mouse γ interferon (MoIFNγ) neutralized the antiviral activity of MoIFNγ but not MoIFNα β, human IFNα (HuIFNα), HuIFNβ or cynomologus monkey IFNγ (CynIFNγ). Comparatively, antibodies to mouse N-terminal synthetic peptide showed only 10% reciprocal cross-reactivity in neutralization tests against heterologous HuIFNγ and 13% cross-reactivity in the ELISA test against Hu N-terminal peptide. The predetermined specificity of these antibodies make them powerful tools for studying antigenic relatedness and biological properties of IFNγs.",

author = "Langford, {Marlyn P.} and Gray, {Patrick W.} and Stanton, {G. John} and Bala Lakhchaura and Chan, {Teh sheng} and Johnson, {Howard M.}",

year = "1983",

month = dec,

day = "28",

doi = "10.1016/0006-291X(83)91676-5",

language = "English (US)",

volume = "117",

pages = "866--871",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "3",

}

TY - JOUR

T1 - Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ)

AU - Langford, Marlyn P.

AU - Gray, Patrick W.

AU - Stanton, G. John

AU - Lakhchaura, Bala

AU - Chan, Teh sheng

AU - Johnson, Howard M.

PY - 1983/12/28

Y1 - 1983/12/28

N2 - Antibodies to an N-terminal synthetic peptide of mouse γ interferon (MoIFNγ) neutralized the antiviral activity of MoIFNγ but not MoIFNα β, human IFNα (HuIFNα), HuIFNβ or cynomologus monkey IFNγ (CynIFNγ). Comparatively, antibodies to mouse N-terminal synthetic peptide showed only 10% reciprocal cross-reactivity in neutralization tests against heterologous HuIFNγ and 13% cross-reactivity in the ELISA test against Hu N-terminal peptide. The predetermined specificity of these antibodies make them powerful tools for studying antigenic relatedness and biological properties of IFNγs.

AB - Antibodies to an N-terminal synthetic peptide of mouse γ interferon (MoIFNγ) neutralized the antiviral activity of MoIFNγ but not MoIFNα β, human IFNα (HuIFNα), HuIFNβ or cynomologus monkey IFNγ (CynIFNγ). Comparatively, antibodies to mouse N-terminal synthetic peptide showed only 10% reciprocal cross-reactivity in neutralization tests against heterologous HuIFNγ and 13% cross-reactivity in the ELISA test against Hu N-terminal peptide. The predetermined specificity of these antibodies make them powerful tools for studying antigenic relatedness and biological properties of IFNγs.

UR - http://www.scopus.com/inward/record.url?scp=0021114584&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021114584&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(83)91676-5

DO - 10.1016/0006-291X(83)91676-5

M3 - Article

C2 - 6421288

AN - SCOPUS:0021114584

SN - 0006-291X

VL - 117

SP - 866

EP - 871

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ)

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this