Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ)

Marlyn P. Langford, Patrick W. Gray, G. John Stanton, Bala Lakhchaura, Teh sheng Chan, Howard M. Johnson

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Antibodies to an N-terminal synthetic peptide of mouse γ interferon (MoIFNγ) neutralized the antiviral activity of MoIFNγ but not MoIFNα β, human IFNα (HuIFNα), HuIFNβ or cynomologus monkey IFNγ (CynIFNγ). Comparatively, antibodies to mouse N-terminal synthetic peptide showed only 10% reciprocal cross-reactivity in neutralization tests against heterologous HuIFNγ and 13% cross-reactivity in the ELISA test against Hu N-terminal peptide. The predetermined specificity of these antibodies make them powerful tools for studying antigenic relatedness and biological properties of IFNγs.

Original languageEnglish (US)
Pages (from-to)866-871
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume117
Issue number3
DOIs
StatePublished - Dec 28 1983

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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