Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ)

Marlyn P. Langford, Patrick W. Gray, G. John Stanton, Bala Lakhchaura, Teh sheng Chan, Howard M. Johnson

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Antibodies to an N-terminal synthetic peptide of mouse γ interferon (MoIFNγ) neutralized the antiviral activity of MoIFNγ but not MoIFNα β, human IFNα (HuIFNα), HuIFNβ or cynomologus monkey IFNγ (CynIFNγ). Comparatively, antibodies to mouse N-terminal synthetic peptide showed only 10% reciprocal cross-reactivity in neutralization tests against heterologous HuIFNγ and 13% cross-reactivity in the ELISA test against Hu N-terminal peptide. The predetermined specificity of these antibodies make them powerful tools for studying antigenic relatedness and biological properties of IFNγs.

Original languageEnglish (US)
Pages (from-to)866-871
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume117
Issue number3
DOIs
StatePublished - Dec 28 1983

Fingerprint

Interferons
Interferon-gamma
Peptides
Antibodies
Neutralization Tests
Antibody Specificity
Haplorhini
Antiviral Agents
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ). / Langford, Marlyn P.; Gray, Patrick W.; Stanton, G. John; Lakhchaura, Bala; Chan, Teh sheng; Johnson, Howard M.

In: Biochemical and Biophysical Research Communications, Vol. 117, No. 3, 28.12.1983, p. 866-871.

Research output: Contribution to journalArticle

Langford, Marlyn P. ; Gray, Patrick W. ; Stanton, G. John ; Lakhchaura, Bala ; Chan, Teh sheng ; Johnson, Howard M. / Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ). In: Biochemical and Biophysical Research Communications. 1983 ; Vol. 117, No. 3. pp. 866-871.
@article{09fb6400f9b740b2aaf26d9c990ea79a,
title = "Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ)",
abstract = "Antibodies to an N-terminal synthetic peptide of mouse γ interferon (MoIFNγ) neutralized the antiviral activity of MoIFNγ but not MoIFNα β, human IFNα (HuIFNα), HuIFNβ or cynomologus monkey IFNγ (CynIFNγ). Comparatively, antibodies to mouse N-terminal synthetic peptide showed only 10{\%} reciprocal cross-reactivity in neutralization tests against heterologous HuIFNγ and 13{\%} cross-reactivity in the ELISA test against Hu N-terminal peptide. The predetermined specificity of these antibodies make them powerful tools for studying antigenic relatedness and biological properties of IFNγs.",
author = "Langford, {Marlyn P.} and Gray, {Patrick W.} and Stanton, {G. John} and Bala Lakhchaura and Chan, {Teh sheng} and Johnson, {Howard M.}",
year = "1983",
month = "12",
day = "28",
doi = "10.1016/0006-291X(83)91676-5",
language = "English (US)",
volume = "117",
pages = "866--871",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ)

AU - Langford, Marlyn P.

AU - Gray, Patrick W.

AU - Stanton, G. John

AU - Lakhchaura, Bala

AU - Chan, Teh sheng

AU - Johnson, Howard M.

PY - 1983/12/28

Y1 - 1983/12/28

N2 - Antibodies to an N-terminal synthetic peptide of mouse γ interferon (MoIFNγ) neutralized the antiviral activity of MoIFNγ but not MoIFNα β, human IFNα (HuIFNα), HuIFNβ or cynomologus monkey IFNγ (CynIFNγ). Comparatively, antibodies to mouse N-terminal synthetic peptide showed only 10% reciprocal cross-reactivity in neutralization tests against heterologous HuIFNγ and 13% cross-reactivity in the ELISA test against Hu N-terminal peptide. The predetermined specificity of these antibodies make them powerful tools for studying antigenic relatedness and biological properties of IFNγs.

AB - Antibodies to an N-terminal synthetic peptide of mouse γ interferon (MoIFNγ) neutralized the antiviral activity of MoIFNγ but not MoIFNα β, human IFNα (HuIFNα), HuIFNβ or cynomologus monkey IFNγ (CynIFNγ). Comparatively, antibodies to mouse N-terminal synthetic peptide showed only 10% reciprocal cross-reactivity in neutralization tests against heterologous HuIFNγ and 13% cross-reactivity in the ELISA test against Hu N-terminal peptide. The predetermined specificity of these antibodies make them powerful tools for studying antigenic relatedness and biological properties of IFNγs.

UR - http://www.scopus.com/inward/record.url?scp=0021114584&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021114584&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(83)91676-5

DO - 10.1016/0006-291X(83)91676-5

M3 - Article

C2 - 6421288

AN - SCOPUS:0021114584

VL - 117

SP - 866

EP - 871

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -