Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ)

Marlyn P. Langford; Patrick W. Gray; G. John Stanton; Bala Lakhchaura; Teh sheng Chan; Howard M. Johnson

doi:10.1016/0006-291X(83)91676-5

Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ)

Marlyn P. Langford, Patrick W. Gray, G. John Stanton, Bala Lakhchaura, Teh sheng Chan, Howard M. Johnson

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Antibodies to an N-terminal synthetic peptide of mouse γ interferon (MoIFNγ) neutralized the antiviral activity of MoIFNγ but not MoIFNα β, human IFNα (HuIFNα), HuIFNβ or cynomologus monkey IFNγ (CynIFNγ). Comparatively, antibodies to mouse N-terminal synthetic peptide showed only 10% reciprocal cross-reactivity in neutralization tests against heterologous HuIFNγ and 13% cross-reactivity in the ELISA test against Hu N-terminal peptide. The predetermined specificity of these antibodies make them powerful tools for studying antigenic relatedness and biological properties of IFNγs.

Original language	English (US)
Pages (from-to)	866-871
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	117
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(83)91676-5
State	Published - Dec 28 1983
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ)",

abstract = "Antibodies to an N-terminal synthetic peptide of mouse γ interferon (MoIFNγ) neutralized the antiviral activity of MoIFNγ but not MoIFNα β, human IFNα (HuIFNα), HuIFNβ or cynomologus monkey IFNγ (CynIFNγ). Comparatively, antibodies to mouse N-terminal synthetic peptide showed only 10% reciprocal cross-reactivity in neutralization tests against heterologous HuIFNγ and 13% cross-reactivity in the ELISA test against Hu N-terminal peptide. The predetermined specificity of these antibodies make them powerful tools for studying antigenic relatedness and biological properties of IFNγs.",

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T1 - Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ)

AU - Langford, Marlyn P.

AU - Gray, Patrick W.

AU - Stanton, G. John

AU - Lakhchaura, Bala

AU - Chan, Teh sheng

AU - Johnson, Howard M.

PY - 1983/12/28

Y1 - 1983/12/28

N2 - Antibodies to an N-terminal synthetic peptide of mouse γ interferon (MoIFNγ) neutralized the antiviral activity of MoIFNγ but not MoIFNα β, human IFNα (HuIFNα), HuIFNβ or cynomologus monkey IFNγ (CynIFNγ). Comparatively, antibodies to mouse N-terminal synthetic peptide showed only 10% reciprocal cross-reactivity in neutralization tests against heterologous HuIFNγ and 13% cross-reactivity in the ELISA test against Hu N-terminal peptide. The predetermined specificity of these antibodies make them powerful tools for studying antigenic relatedness and biological properties of IFNγs.

AB - Antibodies to an N-terminal synthetic peptide of mouse γ interferon (MoIFNγ) neutralized the antiviral activity of MoIFNγ but not MoIFNα β, human IFNα (HuIFNα), HuIFNβ or cynomologus monkey IFNγ (CynIFNγ). Comparatively, antibodies to mouse N-terminal synthetic peptide showed only 10% reciprocal cross-reactivity in neutralization tests against heterologous HuIFNγ and 13% cross-reactivity in the ELISA test against Hu N-terminal peptide. The predetermined specificity of these antibodies make them powerful tools for studying antigenic relatedness and biological properties of IFNγs.

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Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ)

Abstract

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