Abstract
Antibodies to an N-terminal synthetic peptide of mouse γ interferon (MoIFNγ) neutralized the antiviral activity of MoIFNγ but not MoIFNα β, human IFNα (HuIFNα), HuIFNβ or cynomologus monkey IFNγ (CynIFNγ). Comparatively, antibodies to mouse N-terminal synthetic peptide showed only 10% reciprocal cross-reactivity in neutralization tests against heterologous HuIFNγ and 13% cross-reactivity in the ELISA test against Hu N-terminal peptide. The predetermined specificity of these antibodies make them powerful tools for studying antigenic relatedness and biological properties of IFNγs.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 866-871 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 117 |
| Issue number | 3 |
| DOIs | |
| State | Published - Dec 28 1983 |
Fingerprint
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ). / Langford, Marlyn P.; Gray, Patrick W.; Stanton, G. John; Lakhchaura, Bala; Chan, Teh sheng; Johnson, Howard M.
In: Biochemical and Biophysical Research Communications, Vol. 117, No. 3, 28.12.1983, p. 866-871.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFNγ)
AU - Langford, Marlyn P.
AU - Gray, Patrick W.
AU - Stanton, G. John
AU - Lakhchaura, Bala
AU - Chan, Teh sheng
AU - Johnson, Howard M.
PY - 1983/12/28
Y1 - 1983/12/28
N2 - Antibodies to an N-terminal synthetic peptide of mouse γ interferon (MoIFNγ) neutralized the antiviral activity of MoIFNγ but not MoIFNα β, human IFNα (HuIFNα), HuIFNβ or cynomologus monkey IFNγ (CynIFNγ). Comparatively, antibodies to mouse N-terminal synthetic peptide showed only 10% reciprocal cross-reactivity in neutralization tests against heterologous HuIFNγ and 13% cross-reactivity in the ELISA test against Hu N-terminal peptide. The predetermined specificity of these antibodies make them powerful tools for studying antigenic relatedness and biological properties of IFNγs.
AB - Antibodies to an N-terminal synthetic peptide of mouse γ interferon (MoIFNγ) neutralized the antiviral activity of MoIFNγ but not MoIFNα β, human IFNα (HuIFNα), HuIFNβ or cynomologus monkey IFNγ (CynIFNγ). Comparatively, antibodies to mouse N-terminal synthetic peptide showed only 10% reciprocal cross-reactivity in neutralization tests against heterologous HuIFNγ and 13% cross-reactivity in the ELISA test against Hu N-terminal peptide. The predetermined specificity of these antibodies make them powerful tools for studying antigenic relatedness and biological properties of IFNγs.
UR - http://www.scopus.com/inward/record.url?scp=0021114584&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021114584&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(83)91676-5
DO - 10.1016/0006-291X(83)91676-5
M3 - Article
C2 - 6421288
AN - SCOPUS:0021114584
VL - 117
SP - 866
EP - 871
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -