Antibody against purified human hexosaminidase B cross-reacting with human hexosaminidase A

Satish K. Srivastava; Ernest Beutler

doi:10.1016/0006-291X(72)90556-6

Antibody against purified human hexosaminidase B cross-reacting with human hexosaminidase A

Satish K. Srivastava, Ernest Beutler

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article

25 Scopus citations

Abstract

Human hexosaminidase B has been purified to virtually homogeneous state from placenta. An anti-serum has been prepared in rabbits against the purified preparation. The serum reacted equally with human hexosaminidase B (free of hexosaminidase A) and with human hexosaminidase A (free of hexosaminidase B) as shown by immunodiffusion and by precipitation of enzyme activity from solution.

Original language	English (US)
Pages (from-to)	753-759
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	47
Issue number	4
DOIs	https://doi.org/10.1016/0006-291X(72)90556-6
State	Published - May 26 1972

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Srivastava, S. K., & Beutler, E. (1972). Antibody against purified human hexosaminidase B cross-reacting with human hexosaminidase A. Biochemical and Biophysical Research Communications, 47(4), 753-759. https://doi.org/10.1016/0006-291X(72)90556-6

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abstract = "Human hexosaminidase B has been purified to virtually homogeneous state from placenta. An anti-serum has been prepared in rabbits against the purified preparation. The serum reacted equally with human hexosaminidase B (free of hexosaminidase A) and with human hexosaminidase A (free of hexosaminidase B) as shown by immunodiffusion and by precipitation of enzyme activity from solution.",

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N2 - Human hexosaminidase B has been purified to virtually homogeneous state from placenta. An anti-serum has been prepared in rabbits against the purified preparation. The serum reacted equally with human hexosaminidase B (free of hexosaminidase A) and with human hexosaminidase A (free of hexosaminidase B) as shown by immunodiffusion and by precipitation of enzyme activity from solution.

AB - Human hexosaminidase B has been purified to virtually homogeneous state from placenta. An anti-serum has been prepared in rabbits against the purified preparation. The serum reacted equally with human hexosaminidase B (free of hexosaminidase A) and with human hexosaminidase A (free of hexosaminidase B) as shown by immunodiffusion and by precipitation of enzyme activity from solution.

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