Antibody localization in horse, rabbit, and goat antilymphocyte sera

Noboru Kashiwagi, David Sherer, Courtney Townsend, Roberta Jacobs, Keiji Ono, Brij Kapur, Thomas E. Starzl

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The localization of antibodies was studied in rabbit, goat, and horse ALS raised by weekly immunization with canine or human spleen cells for 4 to 12 weeks. A combination of analytic techniques was used including column chromatography, electrophoresis, immunoelectrophoresis, determination of protein concentration, and measurement of antibody titers. In the rabbit and goat ALS, virtually all of the leukoagglutinins and lymphocytotoxins were in the easily separable IgG; accidentally induced thromboagglutinins were in the same location. In the rabbit hemagglutinins were found in both the IgG and IgM, whereas in the goat these were almost exclusively in the IgM. The antiwhite cell antibodies were most widely distributed in the horse. The cytotoxins were primarily in the IgG, but the leukoagglutinins were most heavily concentrated in the T-equine globulin which consists mostly of IgA. By differential ammonium sulfate precipitation of a horse antidoglymphocyte serum, fractions were prepared that were rich in IgG and IgA. Both were able to delay the rejection of canine renal homografts, the IgA-rich preparation to a somewhat greater degree. The findings in this study have been discussed in relation to the refining techniques that have been used for the production of globulin from heterologous ALS.

Original languageEnglish (US)
Pages (from-to)789-798
Number of pages10
JournalSurgery
Volume67
Issue number5
StatePublished - 1970
Externally publishedYes

Fingerprint

Antilymphocyte Serum
Goats
Horses
Immunoglobulin G
Immunoglobulin A
Rabbits
Antibodies
Immunoglobulin M
Canidae
Immunoelectrophoresis
Cytotoxins
Globulins
Ammonium Sulfate
Hemagglutinins
Allografts
Electrophoresis
Chromatography
Immunization
Spleen
Kidney

ASJC Scopus subject areas

  • Surgery

Cite this

Kashiwagi, N., Sherer, D., Townsend, C., Jacobs, R., Ono, K., Kapur, B., & Starzl, T. E. (1970). Antibody localization in horse, rabbit, and goat antilymphocyte sera. Surgery, 67(5), 789-798.

Antibody localization in horse, rabbit, and goat antilymphocyte sera. / Kashiwagi, Noboru; Sherer, David; Townsend, Courtney; Jacobs, Roberta; Ono, Keiji; Kapur, Brij; Starzl, Thomas E.

In: Surgery, Vol. 67, No. 5, 1970, p. 789-798.

Research output: Contribution to journalArticle

Kashiwagi, N, Sherer, D, Townsend, C, Jacobs, R, Ono, K, Kapur, B & Starzl, TE 1970, 'Antibody localization in horse, rabbit, and goat antilymphocyte sera', Surgery, vol. 67, no. 5, pp. 789-798.
Kashiwagi N, Sherer D, Townsend C, Jacobs R, Ono K, Kapur B et al. Antibody localization in horse, rabbit, and goat antilymphocyte sera. Surgery. 1970;67(5):789-798.
Kashiwagi, Noboru ; Sherer, David ; Townsend, Courtney ; Jacobs, Roberta ; Ono, Keiji ; Kapur, Brij ; Starzl, Thomas E. / Antibody localization in horse, rabbit, and goat antilymphocyte sera. In: Surgery. 1970 ; Vol. 67, No. 5. pp. 789-798.
@article{cfe71389cbfc4818a3b65fd8f444c60c,
title = "Antibody localization in horse, rabbit, and goat antilymphocyte sera",
abstract = "The localization of antibodies was studied in rabbit, goat, and horse ALS raised by weekly immunization with canine or human spleen cells for 4 to 12 weeks. A combination of analytic techniques was used including column chromatography, electrophoresis, immunoelectrophoresis, determination of protein concentration, and measurement of antibody titers. In the rabbit and goat ALS, virtually all of the leukoagglutinins and lymphocytotoxins were in the easily separable IgG; accidentally induced thromboagglutinins were in the same location. In the rabbit hemagglutinins were found in both the IgG and IgM, whereas in the goat these were almost exclusively in the IgM. The antiwhite cell antibodies were most widely distributed in the horse. The cytotoxins were primarily in the IgG, but the leukoagglutinins were most heavily concentrated in the T-equine globulin which consists mostly of IgA. By differential ammonium sulfate precipitation of a horse antidoglymphocyte serum, fractions were prepared that were rich in IgG and IgA. Both were able to delay the rejection of canine renal homografts, the IgA-rich preparation to a somewhat greater degree. The findings in this study have been discussed in relation to the refining techniques that have been used for the production of globulin from heterologous ALS.",
author = "Noboru Kashiwagi and David Sherer and Courtney Townsend and Roberta Jacobs and Keiji Ono and Brij Kapur and Starzl, {Thomas E.}",
year = "1970",
language = "English (US)",
volume = "67",
pages = "789--798",
journal = "Surgery",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "5",

}

TY - JOUR

T1 - Antibody localization in horse, rabbit, and goat antilymphocyte sera

AU - Kashiwagi, Noboru

AU - Sherer, David

AU - Townsend, Courtney

AU - Jacobs, Roberta

AU - Ono, Keiji

AU - Kapur, Brij

AU - Starzl, Thomas E.

PY - 1970

Y1 - 1970

N2 - The localization of antibodies was studied in rabbit, goat, and horse ALS raised by weekly immunization with canine or human spleen cells for 4 to 12 weeks. A combination of analytic techniques was used including column chromatography, electrophoresis, immunoelectrophoresis, determination of protein concentration, and measurement of antibody titers. In the rabbit and goat ALS, virtually all of the leukoagglutinins and lymphocytotoxins were in the easily separable IgG; accidentally induced thromboagglutinins were in the same location. In the rabbit hemagglutinins were found in both the IgG and IgM, whereas in the goat these were almost exclusively in the IgM. The antiwhite cell antibodies were most widely distributed in the horse. The cytotoxins were primarily in the IgG, but the leukoagglutinins were most heavily concentrated in the T-equine globulin which consists mostly of IgA. By differential ammonium sulfate precipitation of a horse antidoglymphocyte serum, fractions were prepared that were rich in IgG and IgA. Both were able to delay the rejection of canine renal homografts, the IgA-rich preparation to a somewhat greater degree. The findings in this study have been discussed in relation to the refining techniques that have been used for the production of globulin from heterologous ALS.

AB - The localization of antibodies was studied in rabbit, goat, and horse ALS raised by weekly immunization with canine or human spleen cells for 4 to 12 weeks. A combination of analytic techniques was used including column chromatography, electrophoresis, immunoelectrophoresis, determination of protein concentration, and measurement of antibody titers. In the rabbit and goat ALS, virtually all of the leukoagglutinins and lymphocytotoxins were in the easily separable IgG; accidentally induced thromboagglutinins were in the same location. In the rabbit hemagglutinins were found in both the IgG and IgM, whereas in the goat these were almost exclusively in the IgM. The antiwhite cell antibodies were most widely distributed in the horse. The cytotoxins were primarily in the IgG, but the leukoagglutinins were most heavily concentrated in the T-equine globulin which consists mostly of IgA. By differential ammonium sulfate precipitation of a horse antidoglymphocyte serum, fractions were prepared that were rich in IgG and IgA. Both were able to delay the rejection of canine renal homografts, the IgA-rich preparation to a somewhat greater degree. The findings in this study have been discussed in relation to the refining techniques that have been used for the production of globulin from heterologous ALS.

UR - http://www.scopus.com/inward/record.url?scp=0014780109&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0014780109&partnerID=8YFLogxK

M3 - Article

VL - 67

SP - 789

EP - 798

JO - Surgery

JF - Surgery

SN - 0039-6060

IS - 5

ER -