Antibody production by human colostral cells. I. Immunoglobulin class, specificity, and quantity

S. Ahlstedt, B. Carlsson, L. A. Hanson, R. M. Goldblum

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The production of antibody by human colostral cells was assayed by the hemolysis in gel technique. When sheep erythrocytes coated with O antigens from frequently encountered Escherichia coli bacteria were used as detector cells and anti IgA serum was added for development, numerous plaque forming cells (PFC) were demonstrated in all samples tested. In contrast, plaques were rarely seen in the presence of anti IgG developing serum. The direct (IgM) plaques occasionally noted with both antigen coated and uncoated sheep erythrocytes were mainly due to the production of heterophil antibodies, since they were not formed when human erythrocytes were used as O antigen carriers. A strikingly high number of the colostral lymphocytes formed antibodies to the E. coli antigens, up to 8%. This suggests that these cells represent a rather selective population, possibly cells from the gastrointestinal tract exposed to enteric bacteria. The large number of plaques observed, the predominance of the cells forming IgA antibodies, and the marked changes in PFC number in relationship to parturition raise a number of questions relevant to the antibody producing colostrum cells and their relationship to the secretory immune system.

Original languageEnglish (US)
Pages (from-to)535-539
Number of pages5
JournalScandinavian Journal of Immunology
Volume4
Issue number5-6
StatePublished - 1975
Externally publishedYes

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Immunoglobulin Isotypes
Antibody Formation
O Antigens
Erythrocytes
Sheep
Escherichia coli
Heterophile Antibodies
Antigens
Antibody-Producing Cells
Colostrum
Antibodies
Lymphocyte Count
Enterobacteriaceae
Hemolysis
Serum
Immunoglobulin A
Immunoglobulin M
Gastrointestinal Tract
Immune System
Cell Count

ASJC Scopus subject areas

  • Immunology

Cite this

Ahlstedt, S., Carlsson, B., Hanson, L. A., & Goldblum, R. M. (1975). Antibody production by human colostral cells. I. Immunoglobulin class, specificity, and quantity. Scandinavian Journal of Immunology, 4(5-6), 535-539.

Antibody production by human colostral cells. I. Immunoglobulin class, specificity, and quantity. / Ahlstedt, S.; Carlsson, B.; Hanson, L. A.; Goldblum, R. M.

In: Scandinavian Journal of Immunology, Vol. 4, No. 5-6, 1975, p. 535-539.

Research output: Contribution to journalArticle

Ahlstedt, S, Carlsson, B, Hanson, LA & Goldblum, RM 1975, 'Antibody production by human colostral cells. I. Immunoglobulin class, specificity, and quantity', Scandinavian Journal of Immunology, vol. 4, no. 5-6, pp. 535-539.
Ahlstedt, S. ; Carlsson, B. ; Hanson, L. A. ; Goldblum, R. M. / Antibody production by human colostral cells. I. Immunoglobulin class, specificity, and quantity. In: Scandinavian Journal of Immunology. 1975 ; Vol. 4, No. 5-6. pp. 535-539.
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