Antibody Repertoires to the Same Ebola Vaccine Antigen Are Differentially Affected by Vaccine Vectors

Michelle Meyer, Asuka Yoshida, Palaniappan Ramanathan, Erica Ollmann Saphire, Peter L. Collins, James E. Crowe, Siba Samal, Alexander Bukreyev

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Comparative immune response profiling is important for selecting next-generation vaccines. We comprehensively evaluated the antibody responses from a panel of nine respiratory vaccines against Ebola virus (EBOV) derived from human and avian paramyxoviruses expressing EBOV glycoprotein (GP). Most vaccines were protective in guinea pigs but yielded antibody repertoires that differed in proportion targeting key antigenic regions, avidity, neutralizing antibody specificities, and linear epitope preferences. Competition studies with monoclonal antibodies from human survivors revealed that some epitopes in GP targeted for neutralization were vector dependent, while EBOV-neutralizing titers correlated with the response magnitude toward the receptor-binding domain and GP1/GP2 interface epitopes. While an immunogen determines the breadth of antibody response, distinct vaccine vectors can induce qualitatively different responses, affecting protective efficacy. These data suggest that immune correlates of vaccine protection cannot be generalized for all vaccines against the same pathogen, even if they use the exact same immunogen. Meyer et al. developed a panel of second-generation Ebola vaccines using respiratory viruses to deliver a common immunogen. The vaccines were protective against lethal infection in guinea pigs. The different vaccines elicited different antibody profiles, indicating correlates of protection may not be universal among Ebola vaccines.

    Original languageEnglish (US)
    Pages (from-to)1816-1829
    Number of pages14
    JournalCell Reports
    Volume24
    Issue number7
    DOIs
    StatePublished - Aug 14 2018

    Fingerprint

    Ebola Vaccines
    Vaccines
    Antigens
    Antibodies
    Viruses
    Ebolavirus
    Epitopes
    Antibody Formation
    Avulavirus
    Glycoproteins
    Guinea Pigs
    Antibody Specificity
    Neutralizing Antibodies
    Viral Load
    Pathogens
    Monoclonal Antibodies

    Keywords

    • antibody profile
    • Ebola
    • immune correlates of protection
    • mucosal
    • respiratory
    • vaccine

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

    Cite this

    Antibody Repertoires to the Same Ebola Vaccine Antigen Are Differentially Affected by Vaccine Vectors. / Meyer, Michelle; Yoshida, Asuka; Ramanathan, Palaniappan; Saphire, Erica Ollmann; Collins, Peter L.; Crowe, James E.; Samal, Siba; Bukreyev, Alexander.

    In: Cell Reports, Vol. 24, No. 7, 14.08.2018, p. 1816-1829.

    Research output: Contribution to journalArticle

    Meyer, M, Yoshida, A, Ramanathan, P, Saphire, EO, Collins, PL, Crowe, JE, Samal, S & Bukreyev, A 2018, 'Antibody Repertoires to the Same Ebola Vaccine Antigen Are Differentially Affected by Vaccine Vectors', Cell Reports, vol. 24, no. 7, pp. 1816-1829. https://doi.org/10.1016/j.celrep.2018.07.044
    Meyer M, Yoshida A, Ramanathan P, Saphire EO, Collins PL, Crowe JE et al. Antibody Repertoires to the Same Ebola Vaccine Antigen Are Differentially Affected by Vaccine Vectors. Cell Reports. 2018 Aug 14;24(7):1816-1829. https://doi.org/10.1016/j.celrep.2018.07.044
    Meyer, Michelle ; Yoshida, Asuka ; Ramanathan, Palaniappan ; Saphire, Erica Ollmann ; Collins, Peter L. ; Crowe, James E. ; Samal, Siba ; Bukreyev, Alexander. / Antibody Repertoires to the Same Ebola Vaccine Antigen Are Differentially Affected by Vaccine Vectors. In: Cell Reports. 2018 ; Vol. 24, No. 7. pp. 1816-1829.
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