Anticonvulsant prolongation of survival in adult murine lymphocytic choriomeningitis. II. Ultrastructural observations of pathogenetic events

David Walker, D. L. Camenga, S. Whitfield, F. A. Murphy

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Because in previous ultrastructural studies of murine lymphocytic choriomeningitis (LCM) only mononuclear cell infiltration had been found, without any cytopathology of target cells in the choroid plexus, ependyma, and leptomeninges, diazepam was used to prolong survival for better characterization of late pathologic events. Mice which were treated with diazepam and sacrificed 8, 9, and 10 days after intracerebral inoculation with LCM virus showed an increasing amount of inflammatory infiltration into choroid plexuses, leptomeninges, Virchow Robin spaces, and ependyma. Mononuclear cells, lymphocytes, and polymorphonuclear (PMN) leukocytes increased in number as compared with terminally infected mice sacrificed 7 days after inoculation. Ultrastructurally, choroidal epithelial cells showed cytopathologic changes varying from dilated endoplasmic reticulum through necrosis. Greater numbers of granulocytes, macrophages, and activated macrophages and fewer undifferentiated mononuclear cells were seen in the choroid plexuses of the drug treated survivors. Virions and larger, more numerous arenavirus inclusions were present in the choroid plexus and ependyma. Ultrastructurally the leptomeningitis was characterized by large numbers of activated macrophages. Choroidal epithelial necrosis appears to be the in vivo correlate of T cell mediated cytotoxicity in vitro.

Original languageEnglish (US)
Pages (from-to)21-40
Number of pages20
JournalJournal of Neuropathology and Experimental Neurology
Volume36
Issue number1
StatePublished - 1977
Externally publishedYes

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Lymphocytic Choriomeningitis
Choroid Plexus
Ependyma
Anticonvulsants
Macrophages
Diazepam
Necrosis
Arenavirus
Lymphocytic choriomeningitis virus
Songbirds
Granulocytes
Endoplasmic Reticulum
Virion
Neutrophils
Epithelial Cells
Lymphocytes
T-Lymphocytes
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neuroscience(all)

Cite this

Anticonvulsant prolongation of survival in adult murine lymphocytic choriomeningitis. II. Ultrastructural observations of pathogenetic events. / Walker, David; Camenga, D. L.; Whitfield, S.; Murphy, F. A.

In: Journal of Neuropathology and Experimental Neurology, Vol. 36, No. 1, 1977, p. 21-40.

Research output: Contribution to journalArticle

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