Antidiabetic effects of yam (Dioscorea batatas) and its active constituent, allantoin, in a rat model of streptozotocin-induced diabetes

Hyeon Kyu Go, Mahbubur Rahman, Gi Beum Kim, Chong Sam Na, Choon Ho Song, Jin Shang Kim, Shang Jin Kim, Hyung Sub Kang

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The objective of this study was to investigate the therapeutic efficacies of crude yam (Dioscorea batatas) powder (PY), water extract of yam (EY), and allantoin (the active constituent of yam) in streptozotocin (STZ)-induced diabetic rats with respect to glucose, insulin, glucagon-like peptide-1 (GLP-1), C-peptide, glycated hemoglobin (HbAlc), lipid metabolism, and oxidative stress. For this purpose, 50 rats were divided into five groups: normal control (NC), diabetic control (STZ), and STZ plus treatment groups (STZ + PY, STZ + EY, and STZ + allantoin). After treatment for one-month, there was a decrease in blood glucose: 385 ± 7 in STZ, 231 ± 3 in STZ + PY, 214 ± 11 in STZ + EY, and 243 ± 6 mg/dL in STZ + allantoin, respectively. There were significant statistical differences (p <0.001) compared to STZ (100%): 60% in STZ + PY, 55% in STZ + EY, and 63% in STZ + allantoin. With groups in the same order, there were significant decreases (p <0.001) in HbAlc (100% as 24.4 ± 0.6 ng/mL, 78%, 75%, and 77%), total cholesterol (100% as 122 ± 3 mg/dL, 70%, 67%, and 69%), and low-density lipoprotein (100% as 29 ± 1 mg/dL, 45%, 48%, and 38%). There were also significant increases (p <0.001) in insulin (100% as 0.22 ± 0.00 ng/mL, 173%, 209%, and 177%), GLP-1 (100% as 18.4 ± 0.7 pmol/mL, 160%, 166%, and 162%), and C-peptide (100% as 2.56 ± 0.10 ng/mL, 129%, 132%, and 130%). The treatment effectively ameliorated antioxidant stress as shown by a significant decrease (p <0.001) in malondialdehyde (100% as 7.25 ± 0.11 nmol/mL, 87%, 86%, and 85%) together with increases (p <0.01) in superoxide dismutase (100% as 167 ± 6 IU/mL, 147%, 159%, and 145%) and reduced glutathione (100% as 167 ± 6 nmol/mL, 123%, 141%, and 140%). The results indicate that yam and allantoin have antidiabetic effects by modulating antioxidant activities, lipid profiles and by promoting the release of GLP-1, thereby improving the function of β-cells maintaining normal insulin and glucose levels.

Original languageEnglish (US)
Pages (from-to)8532-8544
Number of pages13
JournalNutrients
Volume7
Issue number10
DOIs
StatePublished - Oct 15 2015
Externally publishedYes

Fingerprint

Dioscorea polystachya
Allantoin
Dioscorea
allantoin
glycemic effect
Experimental Diabetes Mellitus
yams
streptozotocin
Streptozocin
Hypoglycemic Agents
diabetes
animal models
glucagon-like peptide 1
Glucagon-Like Peptide 1
insulin
C-Peptide
extracts
Insulin
Antioxidants
peptides

Keywords

  • Antioxidant
  • Diabetic
  • Dioscorea batatas
  • Glucagon-like peptide-1
  • Hyperglycemia
  • Insulin
  • Rat

ASJC Scopus subject areas

  • Food Science

Cite this

Antidiabetic effects of yam (Dioscorea batatas) and its active constituent, allantoin, in a rat model of streptozotocin-induced diabetes. / Go, Hyeon Kyu; Rahman, Mahbubur; Kim, Gi Beum; Na, Chong Sam; Song, Choon Ho; Kim, Jin Shang; Kim, Shang Jin; Kang, Hyung Sub.

In: Nutrients, Vol. 7, No. 10, 15.10.2015, p. 8532-8544.

Research output: Contribution to journalArticle

Go, Hyeon Kyu ; Rahman, Mahbubur ; Kim, Gi Beum ; Na, Chong Sam ; Song, Choon Ho ; Kim, Jin Shang ; Kim, Shang Jin ; Kang, Hyung Sub. / Antidiabetic effects of yam (Dioscorea batatas) and its active constituent, allantoin, in a rat model of streptozotocin-induced diabetes. In: Nutrients. 2015 ; Vol. 7, No. 10. pp. 8532-8544.
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abstract = "The objective of this study was to investigate the therapeutic efficacies of crude yam (Dioscorea batatas) powder (PY), water extract of yam (EY), and allantoin (the active constituent of yam) in streptozotocin (STZ)-induced diabetic rats with respect to glucose, insulin, glucagon-like peptide-1 (GLP-1), C-peptide, glycated hemoglobin (HbAlc), lipid metabolism, and oxidative stress. For this purpose, 50 rats were divided into five groups: normal control (NC), diabetic control (STZ), and STZ plus treatment groups (STZ + PY, STZ + EY, and STZ + allantoin). After treatment for one-month, there was a decrease in blood glucose: 385 ± 7 in STZ, 231 ± 3 in STZ + PY, 214 ± 11 in STZ + EY, and 243 ± 6 mg/dL in STZ + allantoin, respectively. There were significant statistical differences (p <0.001) compared to STZ (100{\%}): 60{\%} in STZ + PY, 55{\%} in STZ + EY, and 63{\%} in STZ + allantoin. With groups in the same order, there were significant decreases (p <0.001) in HbAlc (100{\%} as 24.4 ± 0.6 ng/mL, 78{\%}, 75{\%}, and 77{\%}), total cholesterol (100{\%} as 122 ± 3 mg/dL, 70{\%}, 67{\%}, and 69{\%}), and low-density lipoprotein (100{\%} as 29 ± 1 mg/dL, 45{\%}, 48{\%}, and 38{\%}). There were also significant increases (p <0.001) in insulin (100{\%} as 0.22 ± 0.00 ng/mL, 173{\%}, 209{\%}, and 177{\%}), GLP-1 (100{\%} as 18.4 ± 0.7 pmol/mL, 160{\%}, 166{\%}, and 162{\%}), and C-peptide (100{\%} as 2.56 ± 0.10 ng/mL, 129{\%}, 132{\%}, and 130{\%}). The treatment effectively ameliorated antioxidant stress as shown by a significant decrease (p <0.001) in malondialdehyde (100{\%} as 7.25 ± 0.11 nmol/mL, 87{\%}, 86{\%}, and 85{\%}) together with increases (p <0.01) in superoxide dismutase (100{\%} as 167 ± 6 IU/mL, 147{\%}, 159{\%}, and 145{\%}) and reduced glutathione (100{\%} as 167 ± 6 nmol/mL, 123{\%}, 141{\%}, and 140{\%}). The results indicate that yam and allantoin have antidiabetic effects by modulating antioxidant activities, lipid profiles and by promoting the release of GLP-1, thereby improving the function of β-cells maintaining normal insulin and glucose levels.",
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T1 - Antidiabetic effects of yam (Dioscorea batatas) and its active constituent, allantoin, in a rat model of streptozotocin-induced diabetes

AU - Go, Hyeon Kyu

AU - Rahman, Mahbubur

AU - Kim, Gi Beum

AU - Na, Chong Sam

AU - Song, Choon Ho

AU - Kim, Jin Shang

AU - Kim, Shang Jin

AU - Kang, Hyung Sub

PY - 2015/10/15

Y1 - 2015/10/15

N2 - The objective of this study was to investigate the therapeutic efficacies of crude yam (Dioscorea batatas) powder (PY), water extract of yam (EY), and allantoin (the active constituent of yam) in streptozotocin (STZ)-induced diabetic rats with respect to glucose, insulin, glucagon-like peptide-1 (GLP-1), C-peptide, glycated hemoglobin (HbAlc), lipid metabolism, and oxidative stress. For this purpose, 50 rats were divided into five groups: normal control (NC), diabetic control (STZ), and STZ plus treatment groups (STZ + PY, STZ + EY, and STZ + allantoin). After treatment for one-month, there was a decrease in blood glucose: 385 ± 7 in STZ, 231 ± 3 in STZ + PY, 214 ± 11 in STZ + EY, and 243 ± 6 mg/dL in STZ + allantoin, respectively. There were significant statistical differences (p <0.001) compared to STZ (100%): 60% in STZ + PY, 55% in STZ + EY, and 63% in STZ + allantoin. With groups in the same order, there were significant decreases (p <0.001) in HbAlc (100% as 24.4 ± 0.6 ng/mL, 78%, 75%, and 77%), total cholesterol (100% as 122 ± 3 mg/dL, 70%, 67%, and 69%), and low-density lipoprotein (100% as 29 ± 1 mg/dL, 45%, 48%, and 38%). There were also significant increases (p <0.001) in insulin (100% as 0.22 ± 0.00 ng/mL, 173%, 209%, and 177%), GLP-1 (100% as 18.4 ± 0.7 pmol/mL, 160%, 166%, and 162%), and C-peptide (100% as 2.56 ± 0.10 ng/mL, 129%, 132%, and 130%). The treatment effectively ameliorated antioxidant stress as shown by a significant decrease (p <0.001) in malondialdehyde (100% as 7.25 ± 0.11 nmol/mL, 87%, 86%, and 85%) together with increases (p <0.01) in superoxide dismutase (100% as 167 ± 6 IU/mL, 147%, 159%, and 145%) and reduced glutathione (100% as 167 ± 6 nmol/mL, 123%, 141%, and 140%). The results indicate that yam and allantoin have antidiabetic effects by modulating antioxidant activities, lipid profiles and by promoting the release of GLP-1, thereby improving the function of β-cells maintaining normal insulin and glucose levels.

AB - The objective of this study was to investigate the therapeutic efficacies of crude yam (Dioscorea batatas) powder (PY), water extract of yam (EY), and allantoin (the active constituent of yam) in streptozotocin (STZ)-induced diabetic rats with respect to glucose, insulin, glucagon-like peptide-1 (GLP-1), C-peptide, glycated hemoglobin (HbAlc), lipid metabolism, and oxidative stress. For this purpose, 50 rats were divided into five groups: normal control (NC), diabetic control (STZ), and STZ plus treatment groups (STZ + PY, STZ + EY, and STZ + allantoin). After treatment for one-month, there was a decrease in blood glucose: 385 ± 7 in STZ, 231 ± 3 in STZ + PY, 214 ± 11 in STZ + EY, and 243 ± 6 mg/dL in STZ + allantoin, respectively. There were significant statistical differences (p <0.001) compared to STZ (100%): 60% in STZ + PY, 55% in STZ + EY, and 63% in STZ + allantoin. With groups in the same order, there were significant decreases (p <0.001) in HbAlc (100% as 24.4 ± 0.6 ng/mL, 78%, 75%, and 77%), total cholesterol (100% as 122 ± 3 mg/dL, 70%, 67%, and 69%), and low-density lipoprotein (100% as 29 ± 1 mg/dL, 45%, 48%, and 38%). There were also significant increases (p <0.001) in insulin (100% as 0.22 ± 0.00 ng/mL, 173%, 209%, and 177%), GLP-1 (100% as 18.4 ± 0.7 pmol/mL, 160%, 166%, and 162%), and C-peptide (100% as 2.56 ± 0.10 ng/mL, 129%, 132%, and 130%). The treatment effectively ameliorated antioxidant stress as shown by a significant decrease (p <0.001) in malondialdehyde (100% as 7.25 ± 0.11 nmol/mL, 87%, 86%, and 85%) together with increases (p <0.01) in superoxide dismutase (100% as 167 ± 6 IU/mL, 147%, 159%, and 145%) and reduced glutathione (100% as 167 ± 6 nmol/mL, 123%, 141%, and 140%). The results indicate that yam and allantoin have antidiabetic effects by modulating antioxidant activities, lipid profiles and by promoting the release of GLP-1, thereby improving the function of β-cells maintaining normal insulin and glucose levels.

KW - Antioxidant

KW - Diabetic

KW - Dioscorea batatas

KW - Glucagon-like peptide-1

KW - Hyperglycemia

KW - Insulin

KW - Rat

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