Antigen capture enzyme-linked immunosorbent assay for specific detection of reston Ebola virus nucleoprotein

Tetsuro Ikegami, Masahiro Niikura, Masayuki Saijo, Mary E. Miranda, Alan B. Calaor, Marvin Hernandez, Luz P. Acosta, Daria L. Manalo, Ichiro Kurane, Yasuhiro Yoshikawa, Shigeru Morikawa

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Antigen capture enzyme-linked immunosorbent assay (ELISA) is one of the most useful methods to detect Ebola virus rapidly. We previously developed an antigen capture ELISA using a monoclonal antibody (MAb), 3-3D, which reacted not only to the nucleoprotein (NP) of Zaire Ebola virus (EBO-Z) but also to the NPs of Sudan (EBO-S) and Reston Ebola (EBO-R) viruses. In this study, we developed antigen capture ELISAs using two novel MAbs, Res2-6C8 and Res2-1D8, specific to the NP of EBO-R. Res2-6C8 and Res2-1D8 recognized epitopes consisting of 4 and 8 amino acid residues, respectively, near the C-terminal region of the EBO-R NP. The antigen capture ELISAs using these two MAbs detected the EBO-R NP in the tissues from EBO-R-infected cynomolgus macaques. The antigen capture ELISAs using Res2-6C8 and Res2-1D8 are useful for the rapid detection of the NP in EBO-R-infected cynomolgus macaques.

Original languageEnglish (US)
Pages (from-to)552-557
Number of pages6
JournalClinical and Diagnostic Laboratory Immunology
Volume10
Issue number4
DOIs
StatePublished - Jul 2003
Externally publishedYes

Fingerprint

Ebolavirus
Immunosorbents
Nucleoproteins
Viruses
Assays
Enzyme-Linked Immunosorbent Assay
Antigens
Enzymes
Macaca
Sudan
rhenium sulfide
Reston
Epitopes
Monoclonal Antibodies
Tissue
Amino Acids

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology and Allergy
  • Clinical Biochemistry
  • Immunology

Cite this

Antigen capture enzyme-linked immunosorbent assay for specific detection of reston Ebola virus nucleoprotein. / Ikegami, Tetsuro; Niikura, Masahiro; Saijo, Masayuki; Miranda, Mary E.; Calaor, Alan B.; Hernandez, Marvin; Acosta, Luz P.; Manalo, Daria L.; Kurane, Ichiro; Yoshikawa, Yasuhiro; Morikawa, Shigeru.

In: Clinical and Diagnostic Laboratory Immunology, Vol. 10, No. 4, 07.2003, p. 552-557.

Research output: Contribution to journalArticle

Ikegami, T, Niikura, M, Saijo, M, Miranda, ME, Calaor, AB, Hernandez, M, Acosta, LP, Manalo, DL, Kurane, I, Yoshikawa, Y & Morikawa, S 2003, 'Antigen capture enzyme-linked immunosorbent assay for specific detection of reston Ebola virus nucleoprotein', Clinical and Diagnostic Laboratory Immunology, vol. 10, no. 4, pp. 552-557. https://doi.org/10.1128/CDLI.10.4.552-557.2003
Ikegami, Tetsuro ; Niikura, Masahiro ; Saijo, Masayuki ; Miranda, Mary E. ; Calaor, Alan B. ; Hernandez, Marvin ; Acosta, Luz P. ; Manalo, Daria L. ; Kurane, Ichiro ; Yoshikawa, Yasuhiro ; Morikawa, Shigeru. / Antigen capture enzyme-linked immunosorbent assay for specific detection of reston Ebola virus nucleoprotein. In: Clinical and Diagnostic Laboratory Immunology. 2003 ; Vol. 10, No. 4. pp. 552-557.
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