Antigen capture enzyme-linked immunosorbent assay for specific detection of reston Ebola virus nucleoprotein

Tetsuro Ikegami; Masahiro Niikura; Masayuki Saijo; Mary E. Miranda; Alan B. Calaor; Marvin Hernandez; Luz P. Acosta; Daria L. Manalo; Ichiro Kurane; Yasuhiro Yoshikawa; Shigeru Morikawa

doi:10.1128/CDLI.10.4.552-557.2003

Antigen capture enzyme-linked immunosorbent assay for specific detection of reston Ebola virus nucleoprotein

Tetsuro Ikegami, Masahiro Niikura, Masayuki Saijo, Mary E. Miranda, Alan B. Calaor, Marvin Hernandez, Luz P. Acosta, Daria L. Manalo, Ichiro Kurane, Yasuhiro Yoshikawa, Shigeru Morikawa

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38 Scopus citations

Abstract

Antigen capture enzyme-linked immunosorbent assay (ELISA) is one of the most useful methods to detect Ebola virus rapidly. We previously developed an antigen capture ELISA using a monoclonal antibody (MAb), 3-3D, which reacted not only to the nucleoprotein (NP) of Zaire Ebola virus (EBO-Z) but also to the NPs of Sudan (EBO-S) and Reston Ebola (EBO-R) viruses. In this study, we developed antigen capture ELISAs using two novel MAbs, Res2-6C8 and Res2-1D8, specific to the NP of EBO-R. Res2-6C8 and Res2-1D8 recognized epitopes consisting of 4 and 8 amino acid residues, respectively, near the C-terminal region of the EBO-R NP. The antigen capture ELISAs using these two MAbs detected the EBO-R NP in the tissues from EBO-R-infected cynomolgus macaques. The antigen capture ELISAs using Res2-6C8 and Res2-1D8 are useful for the rapid detection of the NP in EBO-R-infected cynomolgus macaques.

Original language	English (US)
Pages (from-to)	552-557
Number of pages	6
Journal	Clinical and Diagnostic Laboratory Immunology
Volume	10
Issue number	4
DOIs	https://doi.org/10.1128/CDLI.10.4.552-557.2003
State	Published - Jul 2003
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Clinical Biochemistry
Microbiology (medical)

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10.1128/CDLI.10.4.552-557.2003

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Ikegami, T., Niikura, M., Saijo, M., Miranda, M. E., Calaor, A. B., Hernandez, M., Acosta, L. P., Manalo, D. L., Kurane, I., Yoshikawa, Y., & Morikawa, S. (2003). Antigen capture enzyme-linked immunosorbent assay for specific detection of reston Ebola virus nucleoprotein. Clinical and Diagnostic Laboratory Immunology, 10(4), 552-557. https://doi.org/10.1128/CDLI.10.4.552-557.2003

Ikegami, T, Niikura, M, Saijo, M, Miranda, ME, Calaor, AB, Hernandez, M, Acosta, LP, Manalo, DL, Kurane, I, Yoshikawa, Y & Morikawa, S 2003, 'Antigen capture enzyme-linked immunosorbent assay for specific detection of reston Ebola virus nucleoprotein', Clinical and Diagnostic Laboratory Immunology, vol. 10, no. 4, pp. 552-557. https://doi.org/10.1128/CDLI.10.4.552-557.2003

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abstract = "Antigen capture enzyme-linked immunosorbent assay (ELISA) is one of the most useful methods to detect Ebola virus rapidly. We previously developed an antigen capture ELISA using a monoclonal antibody (MAb), 3-3D, which reacted not only to the nucleoprotein (NP) of Zaire Ebola virus (EBO-Z) but also to the NPs of Sudan (EBO-S) and Reston Ebola (EBO-R) viruses. In this study, we developed antigen capture ELISAs using two novel MAbs, Res2-6C8 and Res2-1D8, specific to the NP of EBO-R. Res2-6C8 and Res2-1D8 recognized epitopes consisting of 4 and 8 amino acid residues, respectively, near the C-terminal region of the EBO-R NP. The antigen capture ELISAs using these two MAbs detected the EBO-R NP in the tissues from EBO-R-infected cynomolgus macaques. The antigen capture ELISAs using Res2-6C8 and Res2-1D8 are useful for the rapid detection of the NP in EBO-R-infected cynomolgus macaques.",

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AU - Saijo, Masayuki

AU - Miranda, Mary E.

AU - Calaor, Alan B.

AU - Hernandez, Marvin

AU - Acosta, Luz P.

AU - Manalo, Daria L.

AU - Kurane, Ichiro

AU - Yoshikawa, Yasuhiro

AU - Morikawa, Shigeru

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N2 - Antigen capture enzyme-linked immunosorbent assay (ELISA) is one of the most useful methods to detect Ebola virus rapidly. We previously developed an antigen capture ELISA using a monoclonal antibody (MAb), 3-3D, which reacted not only to the nucleoprotein (NP) of Zaire Ebola virus (EBO-Z) but also to the NPs of Sudan (EBO-S) and Reston Ebola (EBO-R) viruses. In this study, we developed antigen capture ELISAs using two novel MAbs, Res2-6C8 and Res2-1D8, specific to the NP of EBO-R. Res2-6C8 and Res2-1D8 recognized epitopes consisting of 4 and 8 amino acid residues, respectively, near the C-terminal region of the EBO-R NP. The antigen capture ELISAs using these two MAbs detected the EBO-R NP in the tissues from EBO-R-infected cynomolgus macaques. The antigen capture ELISAs using Res2-6C8 and Res2-1D8 are useful for the rapid detection of the NP in EBO-R-infected cynomolgus macaques.

AB - Antigen capture enzyme-linked immunosorbent assay (ELISA) is one of the most useful methods to detect Ebola virus rapidly. We previously developed an antigen capture ELISA using a monoclonal antibody (MAb), 3-3D, which reacted not only to the nucleoprotein (NP) of Zaire Ebola virus (EBO-Z) but also to the NPs of Sudan (EBO-S) and Reston Ebola (EBO-R) viruses. In this study, we developed antigen capture ELISAs using two novel MAbs, Res2-6C8 and Res2-1D8, specific to the NP of EBO-R. Res2-6C8 and Res2-1D8 recognized epitopes consisting of 4 and 8 amino acid residues, respectively, near the C-terminal region of the EBO-R NP. The antigen capture ELISAs using these two MAbs detected the EBO-R NP in the tissues from EBO-R-infected cynomolgus macaques. The antigen capture ELISAs using Res2-6C8 and Res2-1D8 are useful for the rapid detection of the NP in EBO-R-infected cynomolgus macaques.

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Antigen capture enzyme-linked immunosorbent assay for specific detection of reston Ebola virus nucleoprotein

Abstract

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