Antigenic characterization of flavivirus structural proteins separated by isoelectric focusing

D. W. Trent

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Isoelectrofocusing of nonionic-detergent-disrupted flaviviruses separated the envelope glycoprotein of 53,000 to 58,000 daltons and the nucleocapsid protein of 14,000 daltons. The envelope protein and nucleocapsid protein were isolated at isoelectric points of pI 7.8 and 10.3, respectively. The antigenic determinants of St. Louis encephalitis, Japanese encephalitis, and dengue virus envelope and nucleocapsid proteins were examined by solid-phase competition radioimmunoassay. By the appropriate selection of antiserum and competing proteins, it was possible to distinguish type-specific, complex-reactive and flavivirus group-reactive antigenic determinants. The envelope glycoproteins of St. Louis encephalitis, Japanese encephalitis, and dengue viruses were found to contain each of these three classes of antigenic determinants. Most of the determinants on the envelope protein were type specific, some were complex reactive, and a small fraction were flavivirus group reactive. The nucleocapsid protein contained only flavivirus group-reactive antigenic determinants.

Original languageEnglish (US)
Pages (from-to)608-618
Number of pages11
JournalJournal of Virology
Volume22
Issue number3
StatePublished - 1977

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Flaviviridae
nucleocapsid proteins
Nucleocapsid Proteins
Flavivirus
structural proteins
isoelectric focusing
Isoelectric Focusing
epitopes
Epitopes
St. Louis Encephalitis
Japanese encephalitis virus
Dengue virus
Dengue
encephalitis
Japanese Encephalitis Virus
glycoproteins
Glycoproteins
Proteins
Japanese Encephalitis Viruses
proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Antigenic characterization of flavivirus structural proteins separated by isoelectric focusing. / Trent, D. W.

In: Journal of Virology, Vol. 22, No. 3, 1977, p. 608-618.

Research output: Contribution to journalArticle

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