TY - JOUR
T1 - Antigenic characterization of flavivirus structural proteins separated by isoelectric focusing
AU - Trent, D. W.
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 1977
Y1 - 1977
N2 - Isoelectrofocusing of nonionic-detergent-disrupted flaviviruses separated the envelope glycoprotein of 53,000 to 58,000 daltons and the nucleocapsid protein of 14,000 daltons. The envelope protein and nucleocapsid protein were isolated at isoelectric points of pI 7.8 and 10.3, respectively. The antigenic determinants of St. Louis encephalitis, Japanese encephalitis, and dengue virus envelope and nucleocapsid proteins were examined by solid-phase competition radioimmunoassay. By the appropriate selection of antiserum and competing proteins, it was possible to distinguish type-specific, complex-reactive and flavivirus group-reactive antigenic determinants. The envelope glycoproteins of St. Louis encephalitis, Japanese encephalitis, and dengue viruses were found to contain each of these three classes of antigenic determinants. Most of the determinants on the envelope protein were type specific, some were complex reactive, and a small fraction were flavivirus group reactive. The nucleocapsid protein contained only flavivirus group-reactive antigenic determinants.
AB - Isoelectrofocusing of nonionic-detergent-disrupted flaviviruses separated the envelope glycoprotein of 53,000 to 58,000 daltons and the nucleocapsid protein of 14,000 daltons. The envelope protein and nucleocapsid protein were isolated at isoelectric points of pI 7.8 and 10.3, respectively. The antigenic determinants of St. Louis encephalitis, Japanese encephalitis, and dengue virus envelope and nucleocapsid proteins were examined by solid-phase competition radioimmunoassay. By the appropriate selection of antiserum and competing proteins, it was possible to distinguish type-specific, complex-reactive and flavivirus group-reactive antigenic determinants. The envelope glycoproteins of St. Louis encephalitis, Japanese encephalitis, and dengue viruses were found to contain each of these three classes of antigenic determinants. Most of the determinants on the envelope protein were type specific, some were complex reactive, and a small fraction were flavivirus group reactive. The nucleocapsid protein contained only flavivirus group-reactive antigenic determinants.
UR - http://www.scopus.com/inward/record.url?scp=0017687495&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017687495&partnerID=8YFLogxK
U2 - 10.1128/jvi.22.3.608-618.1977
DO - 10.1128/jvi.22.3.608-618.1977
M3 - Article
C2 - 69040
AN - SCOPUS:0017687495
SN - 0022-538X
VL - 22
SP - 608
EP - 618
JO - Journal of virology
JF - Journal of virology
IS - 3
ER -