Antigenic characterization of the live attenuated Japanese encephalitis vaccine virus SA14-14-2

a comparison with isolates of the virus covering a wide geographic area

Mark R. Wills, Bijon K. Sil, Jing X. Cao, Y. X. Yu, Alan Barrett

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

In China, a live attenuated Japanese encephalitis (JE) vaccine, based on strain SA14-14-2, has been derived from the wild-type strain SA14 as an alternative to the current inactivated vaccines such as Nakayama-NIH and P-3. SA14-14-2 has been characterized using monoclonal antibodies derived in mice, using HAI and neutralization tests, and compared with other Chinese live vaccine clones and 14 wild-type strains of JE virus. Wild-type strain SA14 was found to be a poor immunogen and antigenically distant from all other viruses examined. The vaccine derivatives SA14-5-3 was more immunogenic than its wild-type parent, while SA14-14-2 (derived from SA14-5-3) was more immunogenic than SA14-5-3 and elicited a cross neutralizing antibody response. Our studies indicated that JE virus strain Nakayama elicited as good a neutralizing antibody response in hyperimmunized mice as the SA14-14-2 vaccine clones grown in either primary hamster kidney (PHK) or primary dog kidney (PDK) cells. A single dose of live SA14-14-2 (PHK) also elicited a good antibody response. Antigenic variation between wild-type and vaccine clones of JE virus were detected but were not considered significant in terms of controlling JE virus infections by vaccination.

Original languageEnglish (US)
Pages (from-to)861-872
Number of pages12
JournalVaccine
Volume10
Issue number12
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Japanese Encephalitis Virus
Attenuated Vaccines
Japanese encephalitis virus
encephalitis
Vaccines
vaccines
Viruses
Antibody Formation
viruses
Clone Cells
Neutralizing Antibodies
Kidney
Cricetinae
clones
Japanese Encephalitis Vaccines
neutralizing antibodies
hamsters
Antigenic Variation
Neutralization Tests
Inactivated Vaccines

Keywords

  • Immunogenicity
  • Japanese encephalitis
  • vaccine

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Antigenic characterization of the live attenuated Japanese encephalitis vaccine virus SA14-14-2 : a comparison with isolates of the virus covering a wide geographic area. / Wills, Mark R.; Sil, Bijon K.; Cao, Jing X.; Yu, Y. X.; Barrett, Alan.

In: Vaccine, Vol. 10, No. 12, 1992, p. 861-872.

Research output: Contribution to journalArticle

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