Antigenic diversity of Rickettsia conorii

David Walker, Q. H. Liu, X. J. Yu, H. Li, C. Taylor, H. M. Feng

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Analysis of seven strains designated as Rickettsia conorii for reactivity with a panel of 12 monoclonal antibodies to surface-protein epitopes of spotted fever group rickettsiae and by Western immunoblotting with standard serotyping sera revealed remarkable antigenic diversity. Rickettsial strains from France, Morocco, Ethiopia, Kenya, South Africa, India, and the USSR differed from one another in reactivity with at least one and as many as five monoclonal antibodies. Simko and Indian strains were similar to one another and differed substantially from other R. conorii strains. All seven strains reacted with three R. conorii-specific monoclonal antibodies. Western immunoblotting demonstrated a major 120-kD protein and a major 135-kD protein in all strains. The principal differences were the presence of a major undenatured 130-kD protein in all strains except Indian and Simko, which had an analogous protein of 124 kD. Immunodominant antigenically related, heat- denatured protein bands of 170 kD (Malish 7 strain), 175 kD (Manuel strain), and 190 kD (Kenya tick typhus, Indian, and Simko strains) were not detected in the M-1 and Moroccan strains. This antigenic diversity is greater than that previously reported for other spotted fever group rickettsial species, suggesting that R. conorii is an older species than R. rickettsii with a longer period of time for evolutionary divergence.

Original languageEnglish (US)
Pages (from-to)78-86
Number of pages9
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume47
Issue number1 II SUPPL.
StatePublished - 1992

Fingerprint

Rickettsia conorii
Antigenic Variation
Kenya
Monoclonal Antibodies
Proteins
Fever
Western Blotting
Epidemic Louse-Borne Typhus
Rickettsia
Morocco
Serotyping
Ethiopia
USSR
Ticks
South Africa
France
Epitopes
India
Membrane Proteins
Hot Temperature

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Walker, D., Liu, Q. H., Yu, X. J., Li, H., Taylor, C., & Feng, H. M. (1992). Antigenic diversity of Rickettsia conorii. American Journal of Tropical Medicine and Hygiene, 47(1 II SUPPL.), 78-86.

Antigenic diversity of Rickettsia conorii. / Walker, David; Liu, Q. H.; Yu, X. J.; Li, H.; Taylor, C.; Feng, H. M.

In: American Journal of Tropical Medicine and Hygiene, Vol. 47, No. 1 II SUPPL., 1992, p. 78-86.

Research output: Contribution to journalArticle

Walker, D, Liu, QH, Yu, XJ, Li, H, Taylor, C & Feng, HM 1992, 'Antigenic diversity of Rickettsia conorii', American Journal of Tropical Medicine and Hygiene, vol. 47, no. 1 II SUPPL., pp. 78-86.
Walker D, Liu QH, Yu XJ, Li H, Taylor C, Feng HM. Antigenic diversity of Rickettsia conorii. American Journal of Tropical Medicine and Hygiene. 1992;47(1 II SUPPL.):78-86.
Walker, David ; Liu, Q. H. ; Yu, X. J. ; Li, H. ; Taylor, C. ; Feng, H. M. / Antigenic diversity of Rickettsia conorii. In: American Journal of Tropical Medicine and Hygiene. 1992 ; Vol. 47, No. 1 II SUPPL. pp. 78-86.
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