Antigenic peptide nanofibers elicit adjuvant-free CD8+ T cell responses

Charles B. Chesson, Erica J. Huelsmann, Andrew T. Lacek, Frederick J. Kohlhapp, Matthew F. Webb, Arman Nabatiyan, Andrew Zloza, Jai S. Rudra

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Vaccines that elicit robust CD8+ T cell responses are desirable for protection against infectious diseases and cancers. However, most vaccine adjuvants fail to elicit robust CD8+ T cell responses without inflammation and associated toxicity. We recently reported that self-assembling peptides that form nanofibers in physiological buffers elicited strong adjuvant-free and antigen-specific antibody responses in mice. However, whether or not such nanofibers likewise can elicit strong CD8+ T cell responses is unknown. Here, we demonstrate that the self-assembling peptide Q11 conjugated to a CD8+ T cell epitope of ovalbumin (Q11-OVA), elicits strong antigen-specific primary and recall responses, and in a vaccination regimen protects against subsequent infection. Importantly, we show that these antigenic peptide nanofibers do not persist as an inflammatory antigen depot at the injection site. Our results demonstrate for the first time that self-assembling peptides may be useful as carriers for vaccines where CD8+ T cell-mediated protection is needed.

Original languageEnglish (US)
Pages (from-to)1174-1180
Number of pages7
JournalVaccine
Volume32
Issue number10
DOIs
StatePublished - Feb 26 2014

Fingerprint

nanofibers
Nanofibers
adjuvants
T-lymphocytes
peptides
T-Lymphocytes
Peptides
Vaccines
Antigens
antigens
T-Lymphocyte Epitopes
Cytoprotection
vaccines
Ovalbumin
vaccine adjuvants
injection site
Antibody Formation
Communicable Diseases
ovalbumin
Buffers

Keywords

  • Adjuvant
  • CD8 T cell
  • Nanofiber
  • Peptide
  • Self-assembly
  • Vaccine

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

Cite this

Chesson, C. B., Huelsmann, E. J., Lacek, A. T., Kohlhapp, F. J., Webb, M. F., Nabatiyan, A., ... Rudra, J. S. (2014). Antigenic peptide nanofibers elicit adjuvant-free CD8+ T cell responses. Vaccine, 32(10), 1174-1180. https://doi.org/10.1016/j.vaccine.2013.11.047

Antigenic peptide nanofibers elicit adjuvant-free CD8+ T cell responses. / Chesson, Charles B.; Huelsmann, Erica J.; Lacek, Andrew T.; Kohlhapp, Frederick J.; Webb, Matthew F.; Nabatiyan, Arman; Zloza, Andrew; Rudra, Jai S.

In: Vaccine, Vol. 32, No. 10, 26.02.2014, p. 1174-1180.

Research output: Contribution to journalArticle

Chesson, CB, Huelsmann, EJ, Lacek, AT, Kohlhapp, FJ, Webb, MF, Nabatiyan, A, Zloza, A & Rudra, JS 2014, 'Antigenic peptide nanofibers elicit adjuvant-free CD8+ T cell responses', Vaccine, vol. 32, no. 10, pp. 1174-1180. https://doi.org/10.1016/j.vaccine.2013.11.047
Chesson CB, Huelsmann EJ, Lacek AT, Kohlhapp FJ, Webb MF, Nabatiyan A et al. Antigenic peptide nanofibers elicit adjuvant-free CD8+ T cell responses. Vaccine. 2014 Feb 26;32(10):1174-1180. https://doi.org/10.1016/j.vaccine.2013.11.047
Chesson, Charles B. ; Huelsmann, Erica J. ; Lacek, Andrew T. ; Kohlhapp, Frederick J. ; Webb, Matthew F. ; Nabatiyan, Arman ; Zloza, Andrew ; Rudra, Jai S. / Antigenic peptide nanofibers elicit adjuvant-free CD8+ T cell responses. In: Vaccine. 2014 ; Vol. 32, No. 10. pp. 1174-1180.
@article{84e64165aedd48a18163eee030415443,
title = "Antigenic peptide nanofibers elicit adjuvant-free CD8+ T cell responses",
abstract = "Vaccines that elicit robust CD8+ T cell responses are desirable for protection against infectious diseases and cancers. However, most vaccine adjuvants fail to elicit robust CD8+ T cell responses without inflammation and associated toxicity. We recently reported that self-assembling peptides that form nanofibers in physiological buffers elicited strong adjuvant-free and antigen-specific antibody responses in mice. However, whether or not such nanofibers likewise can elicit strong CD8+ T cell responses is unknown. Here, we demonstrate that the self-assembling peptide Q11 conjugated to a CD8+ T cell epitope of ovalbumin (Q11-OVA), elicits strong antigen-specific primary and recall responses, and in a vaccination regimen protects against subsequent infection. Importantly, we show that these antigenic peptide nanofibers do not persist as an inflammatory antigen depot at the injection site. Our results demonstrate for the first time that self-assembling peptides may be useful as carriers for vaccines where CD8+ T cell-mediated protection is needed.",
keywords = "Adjuvant, CD8 T cell, Nanofiber, Peptide, Self-assembly, Vaccine",
author = "Chesson, {Charles B.} and Huelsmann, {Erica J.} and Lacek, {Andrew T.} and Kohlhapp, {Frederick J.} and Webb, {Matthew F.} and Arman Nabatiyan and Andrew Zloza and Rudra, {Jai S.}",
year = "2014",
month = "2",
day = "26",
doi = "10.1016/j.vaccine.2013.11.047",
language = "English (US)",
volume = "32",
pages = "1174--1180",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "10",

}

TY - JOUR

T1 - Antigenic peptide nanofibers elicit adjuvant-free CD8+ T cell responses

AU - Chesson, Charles B.

AU - Huelsmann, Erica J.

AU - Lacek, Andrew T.

AU - Kohlhapp, Frederick J.

AU - Webb, Matthew F.

AU - Nabatiyan, Arman

AU - Zloza, Andrew

AU - Rudra, Jai S.

PY - 2014/2/26

Y1 - 2014/2/26

N2 - Vaccines that elicit robust CD8+ T cell responses are desirable for protection against infectious diseases and cancers. However, most vaccine adjuvants fail to elicit robust CD8+ T cell responses without inflammation and associated toxicity. We recently reported that self-assembling peptides that form nanofibers in physiological buffers elicited strong adjuvant-free and antigen-specific antibody responses in mice. However, whether or not such nanofibers likewise can elicit strong CD8+ T cell responses is unknown. Here, we demonstrate that the self-assembling peptide Q11 conjugated to a CD8+ T cell epitope of ovalbumin (Q11-OVA), elicits strong antigen-specific primary and recall responses, and in a vaccination regimen protects against subsequent infection. Importantly, we show that these antigenic peptide nanofibers do not persist as an inflammatory antigen depot at the injection site. Our results demonstrate for the first time that self-assembling peptides may be useful as carriers for vaccines where CD8+ T cell-mediated protection is needed.

AB - Vaccines that elicit robust CD8+ T cell responses are desirable for protection against infectious diseases and cancers. However, most vaccine adjuvants fail to elicit robust CD8+ T cell responses without inflammation and associated toxicity. We recently reported that self-assembling peptides that form nanofibers in physiological buffers elicited strong adjuvant-free and antigen-specific antibody responses in mice. However, whether or not such nanofibers likewise can elicit strong CD8+ T cell responses is unknown. Here, we demonstrate that the self-assembling peptide Q11 conjugated to a CD8+ T cell epitope of ovalbumin (Q11-OVA), elicits strong antigen-specific primary and recall responses, and in a vaccination regimen protects against subsequent infection. Importantly, we show that these antigenic peptide nanofibers do not persist as an inflammatory antigen depot at the injection site. Our results demonstrate for the first time that self-assembling peptides may be useful as carriers for vaccines where CD8+ T cell-mediated protection is needed.

KW - Adjuvant

KW - CD8 T cell

KW - Nanofiber

KW - Peptide

KW - Self-assembly

KW - Vaccine

UR - http://www.scopus.com/inward/record.url?scp=84893927702&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84893927702&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2013.11.047

DO - 10.1016/j.vaccine.2013.11.047

M3 - Article

VL - 32

SP - 1174

EP - 1180

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 10

ER -