Antigenic protein modifications in Ehrlichia

S. Thomas, N. Thirumalapura, E. C. Crossley, N. Ismail, David Walker

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To develop effective vaccination strategies against Ehrlichia, we have previously reported developing an animal model of cross-protection in which C57BL/6 mice primed with E. muris were resistant to lethal infection with Ixodes ovatus ehrlichia (IOE). Polyclonal antibody produced in mice after priming with E. muris and later injected with IOE-detected antigenic proteins in E. muris and IOE cell lysates. Cross-reaction of antigenic proteins was observed when we probed both the E. muris and IOE cell lysates with IOE and E. muris-specific polyclonal antibody. Analysis of the total proteins of E. muris and IOE by two dimensional electrophoresis showed that both E. muris and IOE have the same antigenic proteins. Finally, studies on post-translational protein modifications using a novel technique, Eastern blotting, showed that E. muris proteins are more lipoylated and glycosylated than those of IOE.

Original languageEnglish (US)
Pages (from-to)296-303
Number of pages8
JournalParasite Immunology
Volume31
Issue number6
DOIs
StatePublished - Jun 2009

Fingerprint

Ehrlichia
Ixodes
Proteins
Cross Protection
Antibodies
Cross Reactions
Post Translational Protein Processing
Inbred C57BL Mouse
Electrophoresis
Vaccination
Animal Models

Keywords

  • Antigen
  • Eastern blotting
  • Ehrlichia
  • Ehrlichiosis
  • IOE

ASJC Scopus subject areas

  • Parasitology
  • Immunology

Cite this

Thomas, S., Thirumalapura, N., Crossley, E. C., Ismail, N., & Walker, D. (2009). Antigenic protein modifications in Ehrlichia. Parasite Immunology, 31(6), 296-303. https://doi.org/10.1111/j.1365-3024.2009.01099.x

Antigenic protein modifications in Ehrlichia. / Thomas, S.; Thirumalapura, N.; Crossley, E. C.; Ismail, N.; Walker, David.

In: Parasite Immunology, Vol. 31, No. 6, 06.2009, p. 296-303.

Research output: Contribution to journalArticle

Thomas, S, Thirumalapura, N, Crossley, EC, Ismail, N & Walker, D 2009, 'Antigenic protein modifications in Ehrlichia', Parasite Immunology, vol. 31, no. 6, pp. 296-303. https://doi.org/10.1111/j.1365-3024.2009.01099.x
Thomas, S. ; Thirumalapura, N. ; Crossley, E. C. ; Ismail, N. ; Walker, David. / Antigenic protein modifications in Ehrlichia. In: Parasite Immunology. 2009 ; Vol. 31, No. 6. pp. 296-303.
@article{2a56368b3c704c7aa10b0f552c068f6e,
title = "Antigenic protein modifications in Ehrlichia",
abstract = "To develop effective vaccination strategies against Ehrlichia, we have previously reported developing an animal model of cross-protection in which C57BL/6 mice primed with E. muris were resistant to lethal infection with Ixodes ovatus ehrlichia (IOE). Polyclonal antibody produced in mice after priming with E. muris and later injected with IOE-detected antigenic proteins in E. muris and IOE cell lysates. Cross-reaction of antigenic proteins was observed when we probed both the E. muris and IOE cell lysates with IOE and E. muris-specific polyclonal antibody. Analysis of the total proteins of E. muris and IOE by two dimensional electrophoresis showed that both E. muris and IOE have the same antigenic proteins. Finally, studies on post-translational protein modifications using a novel technique, Eastern blotting, showed that E. muris proteins are more lipoylated and glycosylated than those of IOE.",
keywords = "Antigen, Eastern blotting, Ehrlichia, Ehrlichiosis, IOE",
author = "S. Thomas and N. Thirumalapura and Crossley, {E. C.} and N. Ismail and David Walker",
year = "2009",
month = "6",
doi = "10.1111/j.1365-3024.2009.01099.x",
language = "English (US)",
volume = "31",
pages = "296--303",
journal = "Parasite Immunology",
issn = "0141-9838",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Antigenic protein modifications in Ehrlichia

AU - Thomas, S.

AU - Thirumalapura, N.

AU - Crossley, E. C.

AU - Ismail, N.

AU - Walker, David

PY - 2009/6

Y1 - 2009/6

N2 - To develop effective vaccination strategies against Ehrlichia, we have previously reported developing an animal model of cross-protection in which C57BL/6 mice primed with E. muris were resistant to lethal infection with Ixodes ovatus ehrlichia (IOE). Polyclonal antibody produced in mice after priming with E. muris and later injected with IOE-detected antigenic proteins in E. muris and IOE cell lysates. Cross-reaction of antigenic proteins was observed when we probed both the E. muris and IOE cell lysates with IOE and E. muris-specific polyclonal antibody. Analysis of the total proteins of E. muris and IOE by two dimensional electrophoresis showed that both E. muris and IOE have the same antigenic proteins. Finally, studies on post-translational protein modifications using a novel technique, Eastern blotting, showed that E. muris proteins are more lipoylated and glycosylated than those of IOE.

AB - To develop effective vaccination strategies against Ehrlichia, we have previously reported developing an animal model of cross-protection in which C57BL/6 mice primed with E. muris were resistant to lethal infection with Ixodes ovatus ehrlichia (IOE). Polyclonal antibody produced in mice after priming with E. muris and later injected with IOE-detected antigenic proteins in E. muris and IOE cell lysates. Cross-reaction of antigenic proteins was observed when we probed both the E. muris and IOE cell lysates with IOE and E. muris-specific polyclonal antibody. Analysis of the total proteins of E. muris and IOE by two dimensional electrophoresis showed that both E. muris and IOE have the same antigenic proteins. Finally, studies on post-translational protein modifications using a novel technique, Eastern blotting, showed that E. muris proteins are more lipoylated and glycosylated than those of IOE.

KW - Antigen

KW - Eastern blotting

KW - Ehrlichia

KW - Ehrlichiosis

KW - IOE

UR - http://www.scopus.com/inward/record.url?scp=66449102184&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=66449102184&partnerID=8YFLogxK

U2 - 10.1111/j.1365-3024.2009.01099.x

DO - 10.1111/j.1365-3024.2009.01099.x

M3 - Article

VL - 31

SP - 296

EP - 303

JO - Parasite Immunology

JF - Parasite Immunology

SN - 0141-9838

IS - 6

ER -