Antigenic protein modifications in Ehrlichia

S. Thomas, N. Thirumalapura, E. C. Crossley, N. Ismail, D. H. Walker

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

To develop effective vaccination strategies against Ehrlichia, we have previously reported developing an animal model of cross-protection in which C57BL/6 mice primed with E. muris were resistant to lethal infection with Ixodes ovatus ehrlichia (IOE). Polyclonal antibody produced in mice after priming with E. muris and later injected with IOE-detected antigenic proteins in E. muris and IOE cell lysates. Cross-reaction of antigenic proteins was observed when we probed both the E. muris and IOE cell lysates with IOE and E. muris-specific polyclonal antibody. Analysis of the total proteins of E. muris and IOE by two dimensional electrophoresis showed that both E. muris and IOE have the same antigenic proteins. Finally, studies on post-translational protein modifications using a novel technique, Eastern blotting, showed that E. muris proteins are more lipoylated and glycosylated than those of IOE.

Original languageEnglish (US)
Pages (from-to)296-303
Number of pages8
JournalParasite Immunology
Volume31
Issue number6
DOIs
StatePublished - Jun 2009

Keywords

  • Antigen
  • Eastern blotting
  • Ehrlichia
  • Ehrlichiosis
  • IOE

ASJC Scopus subject areas

  • Parasitology
  • Immunology

Fingerprint Dive into the research topics of 'Antigenic protein modifications in Ehrlichia'. Together they form a unique fingerprint.

  • Cite this

    Thomas, S., Thirumalapura, N., Crossley, E. C., Ismail, N., & Walker, D. H. (2009). Antigenic protein modifications in Ehrlichia. Parasite Immunology, 31(6), 296-303. https://doi.org/10.1111/j.1365-3024.2009.01099.x