Antigenic protein modifications in Ehrlichia

S. Thomas; N. Thirumalapura; E. C. Crossley; N. Ismail; D. H. Walker

doi:10.1111/j.1365-3024.2009.01099.x

Antigenic protein modifications in Ehrlichia

S. Thomas, N. Thirumalapura, E. C. Crossley, N. Ismail, D. H. Walker

Pathology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

To develop effective vaccination strategies against Ehrlichia, we have previously reported developing an animal model of cross-protection in which C57BL/6 mice primed with E. muris were resistant to lethal infection with Ixodes ovatus ehrlichia (IOE). Polyclonal antibody produced in mice after priming with E. muris and later injected with IOE-detected antigenic proteins in E. muris and IOE cell lysates. Cross-reaction of antigenic proteins was observed when we probed both the E. muris and IOE cell lysates with IOE and E. muris-specific polyclonal antibody. Analysis of the total proteins of E. muris and IOE by two dimensional electrophoresis showed that both E. muris and IOE have the same antigenic proteins. Finally, studies on post-translational protein modifications using a novel technique, Eastern blotting, showed that E. muris proteins are more lipoylated and glycosylated than those of IOE.

Original language	English (US)
Pages (from-to)	296-303
Number of pages	8
Journal	Parasite Immunology
Volume	31
Issue number	6
DOIs	https://doi.org/10.1111/j.1365-3024.2009.01099.x
State	Published - Jun 2009

Keywords

Antigen
Eastern blotting
Ehrlichia
Ehrlichiosis
IOE

ASJC Scopus subject areas

Parasitology
Immunology

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10.1111/j.1365-3024.2009.01099.x

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title = "Antigenic protein modifications in Ehrlichia",

abstract = "To develop effective vaccination strategies against Ehrlichia, we have previously reported developing an animal model of cross-protection in which C57BL/6 mice primed with E. muris were resistant to lethal infection with Ixodes ovatus ehrlichia (IOE). Polyclonal antibody produced in mice after priming with E. muris and later injected with IOE-detected antigenic proteins in E. muris and IOE cell lysates. Cross-reaction of antigenic proteins was observed when we probed both the E. muris and IOE cell lysates with IOE and E. muris-specific polyclonal antibody. Analysis of the total proteins of E. muris and IOE by two dimensional electrophoresis showed that both E. muris and IOE have the same antigenic proteins. Finally, studies on post-translational protein modifications using a novel technique, Eastern blotting, showed that E. muris proteins are more lipoylated and glycosylated than those of IOE.",

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AU - Thirumalapura, N.

AU - Crossley, E. C.

AU - Ismail, N.

AU - Walker, D. H.

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N2 - To develop effective vaccination strategies against Ehrlichia, we have previously reported developing an animal model of cross-protection in which C57BL/6 mice primed with E. muris were resistant to lethal infection with Ixodes ovatus ehrlichia (IOE). Polyclonal antibody produced in mice after priming with E. muris and later injected with IOE-detected antigenic proteins in E. muris and IOE cell lysates. Cross-reaction of antigenic proteins was observed when we probed both the E. muris and IOE cell lysates with IOE and E. muris-specific polyclonal antibody. Analysis of the total proteins of E. muris and IOE by two dimensional electrophoresis showed that both E. muris and IOE have the same antigenic proteins. Finally, studies on post-translational protein modifications using a novel technique, Eastern blotting, showed that E. muris proteins are more lipoylated and glycosylated than those of IOE.

AB - To develop effective vaccination strategies against Ehrlichia, we have previously reported developing an animal model of cross-protection in which C57BL/6 mice primed with E. muris were resistant to lethal infection with Ixodes ovatus ehrlichia (IOE). Polyclonal antibody produced in mice after priming with E. muris and later injected with IOE-detected antigenic proteins in E. muris and IOE cell lysates. Cross-reaction of antigenic proteins was observed when we probed both the E. muris and IOE cell lysates with IOE and E. muris-specific polyclonal antibody. Analysis of the total proteins of E. muris and IOE by two dimensional electrophoresis showed that both E. muris and IOE have the same antigenic proteins. Finally, studies on post-translational protein modifications using a novel technique, Eastern blotting, showed that E. muris proteins are more lipoylated and glycosylated than those of IOE.

KW - Antigen

KW - Eastern blotting

KW - Ehrlichia

KW - Ehrlichiosis

KW - IOE

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Antigenic protein modifications in Ehrlichia

Abstract

Keywords

ASJC Scopus subject areas

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