Antimicrobial resistance and molecular typing of Staphylococcus aureus bloodstream isolates from hospitals in Peru

Coralith García, Michelle I A Rijnders, Cathrien Bruggeman, Frine Samalvides, Ellen E. Stobberingh, Jan Jacobs

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) infections are of worldwide concern. The present study describes the antimicrobial resistance and molecular typing of methicillin-resistant and methicillin-susceptible S. aureus (MSSA) bloodstream isolates in Peru. Methods: Consecutive non-duplicate S. aureus bloodstream isolates were collected over a 15-month period (2008-2009) from seven hospitals in Lima and Callao, two contiguous cities in Peru. Detection of mecA gene, spa typing and Staphylococcal Chromosomal Cassette (SCC). mec typing were performed. Antimicrobial resistance was assessed by disk diffusion. Results: Of 338 isolates, MRSA rate was 50.0%. Among MRSA isolates (n = 169), 81.7% were associated to MLST CC5, 68.8% had spa t149/SCC. mec I, and more than 85% were co-resistant to ciprofloxacin, clindamycin, erythromycin and gentamicin; 8.9% (n = 15) were associated to MLST CC8, 14 of them had spa t148/SCC. mec IV, and more than 70% were co-resistant to ciprofloxacin, clindamycin and erythromycin. Among MSSA isolates (n = 169), there was a higher diversity of spa types (n = 56) compared to MRSA isolates (n = 17), 27.2% were associated to MLST CC8, 23.7% were resistant to erythromycin and clindamycin resistance exceeded 20%. Conclusions: MRSA rate among bloodstream isolates in Peru was 50%, with MLST CC5/t149/SCC. mec I representing the most frequent clone.

Original languageEnglish (US)
Pages (from-to)406-411
Number of pages6
JournalJournal of Infection
Volume65
Issue number5
DOIs
StatePublished - Nov 2012
Externally publishedYes

Fingerprint

Molecular Typing
Peru
Methicillin-Resistant Staphylococcus aureus
Staphylococcus aureus
Clindamycin
Erythromycin
Methicillin
Ciprofloxacin
Methicillin Resistance
Gentamicins
Clone Cells
Infection
Genes

Keywords

  • Latin America
  • Molecular characteristics
  • Peru
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

García, C., Rijnders, M. I. A., Bruggeman, C., Samalvides, F., Stobberingh, E. E., & Jacobs, J. (2012). Antimicrobial resistance and molecular typing of Staphylococcus aureus bloodstream isolates from hospitals in Peru. Journal of Infection, 65(5), 406-411. https://doi.org/10.1016/j.jinf.2012.06.009

Antimicrobial resistance and molecular typing of Staphylococcus aureus bloodstream isolates from hospitals in Peru. / García, Coralith; Rijnders, Michelle I A; Bruggeman, Cathrien; Samalvides, Frine; Stobberingh, Ellen E.; Jacobs, Jan.

In: Journal of Infection, Vol. 65, No. 5, 11.2012, p. 406-411.

Research output: Contribution to journalArticle

García, C, Rijnders, MIA, Bruggeman, C, Samalvides, F, Stobberingh, EE & Jacobs, J 2012, 'Antimicrobial resistance and molecular typing of Staphylococcus aureus bloodstream isolates from hospitals in Peru', Journal of Infection, vol. 65, no. 5, pp. 406-411. https://doi.org/10.1016/j.jinf.2012.06.009
García, Coralith ; Rijnders, Michelle I A ; Bruggeman, Cathrien ; Samalvides, Frine ; Stobberingh, Ellen E. ; Jacobs, Jan. / Antimicrobial resistance and molecular typing of Staphylococcus aureus bloodstream isolates from hospitals in Peru. In: Journal of Infection. 2012 ; Vol. 65, No. 5. pp. 406-411.
@article{275044892860426091167ba63561f76e,
title = "Antimicrobial resistance and molecular typing of Staphylococcus aureus bloodstream isolates from hospitals in Peru",
abstract = "Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) infections are of worldwide concern. The present study describes the antimicrobial resistance and molecular typing of methicillin-resistant and methicillin-susceptible S. aureus (MSSA) bloodstream isolates in Peru. Methods: Consecutive non-duplicate S. aureus bloodstream isolates were collected over a 15-month period (2008-2009) from seven hospitals in Lima and Callao, two contiguous cities in Peru. Detection of mecA gene, spa typing and Staphylococcal Chromosomal Cassette (SCC). mec typing were performed. Antimicrobial resistance was assessed by disk diffusion. Results: Of 338 isolates, MRSA rate was 50.0{\%}. Among MRSA isolates (n = 169), 81.7{\%} were associated to MLST CC5, 68.8{\%} had spa t149/SCC. mec I, and more than 85{\%} were co-resistant to ciprofloxacin, clindamycin, erythromycin and gentamicin; 8.9{\%} (n = 15) were associated to MLST CC8, 14 of them had spa t148/SCC. mec IV, and more than 70{\%} were co-resistant to ciprofloxacin, clindamycin and erythromycin. Among MSSA isolates (n = 169), there was a higher diversity of spa types (n = 56) compared to MRSA isolates (n = 17), 27.2{\%} were associated to MLST CC8, 23.7{\%} were resistant to erythromycin and clindamycin resistance exceeded 20{\%}. Conclusions: MRSA rate among bloodstream isolates in Peru was 50{\%}, with MLST CC5/t149/SCC. mec I representing the most frequent clone.",
keywords = "Latin America, Molecular characteristics, Peru, Staphylococcus aureus",
author = "Coralith Garc{\'i}a and Rijnders, {Michelle I A} and Cathrien Bruggeman and Frine Samalvides and Stobberingh, {Ellen E.} and Jan Jacobs",
year = "2012",
month = "11",
doi = "10.1016/j.jinf.2012.06.009",
language = "English (US)",
volume = "65",
pages = "406--411",
journal = "Journal of Infection",
issn = "0163-4453",
publisher = "W.B. Saunders Ltd",
number = "5",

}

TY - JOUR

T1 - Antimicrobial resistance and molecular typing of Staphylococcus aureus bloodstream isolates from hospitals in Peru

AU - García, Coralith

AU - Rijnders, Michelle I A

AU - Bruggeman, Cathrien

AU - Samalvides, Frine

AU - Stobberingh, Ellen E.

AU - Jacobs, Jan

PY - 2012/11

Y1 - 2012/11

N2 - Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) infections are of worldwide concern. The present study describes the antimicrobial resistance and molecular typing of methicillin-resistant and methicillin-susceptible S. aureus (MSSA) bloodstream isolates in Peru. Methods: Consecutive non-duplicate S. aureus bloodstream isolates were collected over a 15-month period (2008-2009) from seven hospitals in Lima and Callao, two contiguous cities in Peru. Detection of mecA gene, spa typing and Staphylococcal Chromosomal Cassette (SCC). mec typing were performed. Antimicrobial resistance was assessed by disk diffusion. Results: Of 338 isolates, MRSA rate was 50.0%. Among MRSA isolates (n = 169), 81.7% were associated to MLST CC5, 68.8% had spa t149/SCC. mec I, and more than 85% were co-resistant to ciprofloxacin, clindamycin, erythromycin and gentamicin; 8.9% (n = 15) were associated to MLST CC8, 14 of them had spa t148/SCC. mec IV, and more than 70% were co-resistant to ciprofloxacin, clindamycin and erythromycin. Among MSSA isolates (n = 169), there was a higher diversity of spa types (n = 56) compared to MRSA isolates (n = 17), 27.2% were associated to MLST CC8, 23.7% were resistant to erythromycin and clindamycin resistance exceeded 20%. Conclusions: MRSA rate among bloodstream isolates in Peru was 50%, with MLST CC5/t149/SCC. mec I representing the most frequent clone.

AB - Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) infections are of worldwide concern. The present study describes the antimicrobial resistance and molecular typing of methicillin-resistant and methicillin-susceptible S. aureus (MSSA) bloodstream isolates in Peru. Methods: Consecutive non-duplicate S. aureus bloodstream isolates were collected over a 15-month period (2008-2009) from seven hospitals in Lima and Callao, two contiguous cities in Peru. Detection of mecA gene, spa typing and Staphylococcal Chromosomal Cassette (SCC). mec typing were performed. Antimicrobial resistance was assessed by disk diffusion. Results: Of 338 isolates, MRSA rate was 50.0%. Among MRSA isolates (n = 169), 81.7% were associated to MLST CC5, 68.8% had spa t149/SCC. mec I, and more than 85% were co-resistant to ciprofloxacin, clindamycin, erythromycin and gentamicin; 8.9% (n = 15) were associated to MLST CC8, 14 of them had spa t148/SCC. mec IV, and more than 70% were co-resistant to ciprofloxacin, clindamycin and erythromycin. Among MSSA isolates (n = 169), there was a higher diversity of spa types (n = 56) compared to MRSA isolates (n = 17), 27.2% were associated to MLST CC8, 23.7% were resistant to erythromycin and clindamycin resistance exceeded 20%. Conclusions: MRSA rate among bloodstream isolates in Peru was 50%, with MLST CC5/t149/SCC. mec I representing the most frequent clone.

KW - Latin America

KW - Molecular characteristics

KW - Peru

KW - Staphylococcus aureus

UR - http://www.scopus.com/inward/record.url?scp=84867335026&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867335026&partnerID=8YFLogxK

U2 - 10.1016/j.jinf.2012.06.009

DO - 10.1016/j.jinf.2012.06.009

M3 - Article

C2 - 22750236

AN - SCOPUS:84867335026

VL - 65

SP - 406

EP - 411

JO - Journal of Infection

JF - Journal of Infection

SN - 0163-4453

IS - 5

ER -