TY - JOUR
T1 - Antimicrobial resistance and molecular typing of Staphylococcus aureus bloodstream isolates from hospitals in Peru
AU - García, Coralith
AU - Rijnders, Michelle I.A.
AU - Bruggeman, Cathrien
AU - Samalvides, Frine
AU - Stobberingh, Ellen E.
AU - Jacobs, Jan
PY - 2012/11
Y1 - 2012/11
N2 - Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) infections are of worldwide concern. The present study describes the antimicrobial resistance and molecular typing of methicillin-resistant and methicillin-susceptible S. aureus (MSSA) bloodstream isolates in Peru. Methods: Consecutive non-duplicate S. aureus bloodstream isolates were collected over a 15-month period (2008-2009) from seven hospitals in Lima and Callao, two contiguous cities in Peru. Detection of mecA gene, spa typing and Staphylococcal Chromosomal Cassette (SCC). mec typing were performed. Antimicrobial resistance was assessed by disk diffusion. Results: Of 338 isolates, MRSA rate was 50.0%. Among MRSA isolates (n = 169), 81.7% were associated to MLST CC5, 68.8% had spa t149/SCC. mec I, and more than 85% were co-resistant to ciprofloxacin, clindamycin, erythromycin and gentamicin; 8.9% (n = 15) were associated to MLST CC8, 14 of them had spa t148/SCC. mec IV, and more than 70% were co-resistant to ciprofloxacin, clindamycin and erythromycin. Among MSSA isolates (n = 169), there was a higher diversity of spa types (n = 56) compared to MRSA isolates (n = 17), 27.2% were associated to MLST CC8, 23.7% were resistant to erythromycin and clindamycin resistance exceeded 20%. Conclusions: MRSA rate among bloodstream isolates in Peru was 50%, with MLST CC5/t149/SCC. mec I representing the most frequent clone.
AB - Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) infections are of worldwide concern. The present study describes the antimicrobial resistance and molecular typing of methicillin-resistant and methicillin-susceptible S. aureus (MSSA) bloodstream isolates in Peru. Methods: Consecutive non-duplicate S. aureus bloodstream isolates were collected over a 15-month period (2008-2009) from seven hospitals in Lima and Callao, two contiguous cities in Peru. Detection of mecA gene, spa typing and Staphylococcal Chromosomal Cassette (SCC). mec typing were performed. Antimicrobial resistance was assessed by disk diffusion. Results: Of 338 isolates, MRSA rate was 50.0%. Among MRSA isolates (n = 169), 81.7% were associated to MLST CC5, 68.8% had spa t149/SCC. mec I, and more than 85% were co-resistant to ciprofloxacin, clindamycin, erythromycin and gentamicin; 8.9% (n = 15) were associated to MLST CC8, 14 of them had spa t148/SCC. mec IV, and more than 70% were co-resistant to ciprofloxacin, clindamycin and erythromycin. Among MSSA isolates (n = 169), there was a higher diversity of spa types (n = 56) compared to MRSA isolates (n = 17), 27.2% were associated to MLST CC8, 23.7% were resistant to erythromycin and clindamycin resistance exceeded 20%. Conclusions: MRSA rate among bloodstream isolates in Peru was 50%, with MLST CC5/t149/SCC. mec I representing the most frequent clone.
KW - Latin America
KW - Molecular characteristics
KW - Peru
KW - Staphylococcus aureus
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U2 - 10.1016/j.jinf.2012.06.009
DO - 10.1016/j.jinf.2012.06.009
M3 - Article
C2 - 22750236
AN - SCOPUS:84867335026
SN - 0163-4453
VL - 65
SP - 406
EP - 411
JO - Journal of Infection
JF - Journal of Infection
IS - 5
ER -