Antioxidant effect of retinoic acid on PC12 rat pheochromocytoma

George R. Jackson, Brent C. Morgan, Karin Werrbach-Perez, J. Regino Perez-Polo

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    Retinoic acid is a naturally occurring metabolite of vitamin A that influences the differentiation of a variety of neural cells in vitro. In the LA-N-1 human neuroblastoma line, retinoic acid treatment increases the binding of nerve growth factor (Bmax). The purpose of this study was to examine the effects of retinoic acid on PC12 rat pheochromocytoma, a neural crest-derived cell line that can be induced to express a sympathetic neuroblast-like phenotype by nerve growth factor treatment. In contrast to the differentiating effects of nerve growth factor, retinoic acid treatment of PC12 cells had a negligible effect on cellular morphology. However, treatment with retinoic acid enhanced the survival of PC12 cells following oxidative injury generated by H2O2 treatment in a manner that is qualitatively similar to that observed after nerve growth factor treatment. Also, there was an increase in 125I-nerve growth factor binding activity in solubilized PC12 membrane preparations derived from retinoic acid-treated PC12 cells. These data suggest that retinoic acid may play a role in neuronal development and in neuronal injury by stimulating the ability of neurons to cope with oxidative stress and/or by enhancing neuronal responsiveness to trophic factors such as the nerve growth factor.

    Original languageEnglish (US)
    JournalInternational Journal of Developmental Neuroscience
    Volume9
    Issue number2
    DOIs
    StatePublished - 1991

    Fingerprint

    Pheochromocytoma
    Tretinoin
    Nerve Growth Factor
    Antioxidants
    PC12 Cells
    Neural Crest
    Wounds and Injuries
    Vitamin A
    Neuroblastoma
    Oxidative Stress
    Phenotype
    Neurons
    Cell Line
    Membranes

    Keywords

    • antioxidant
    • hydrogen peroxide
    • nerve growth factor receptors
    • PC12
    • retinoic acid

    ASJC Scopus subject areas

    • Developmental Biology
    • Developmental Neuroscience

    Cite this

    Antioxidant effect of retinoic acid on PC12 rat pheochromocytoma. / Jackson, George R.; Morgan, Brent C.; Werrbach-Perez, Karin; Perez-Polo, J. Regino.

    In: International Journal of Developmental Neuroscience, Vol. 9, No. 2, 1991.

    Research output: Contribution to journalArticle

    Jackson, George R. ; Morgan, Brent C. ; Werrbach-Perez, Karin ; Perez-Polo, J. Regino. / Antioxidant effect of retinoic acid on PC12 rat pheochromocytoma. In: International Journal of Developmental Neuroscience. 1991 ; Vol. 9, No. 2.
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