Antioxidant enzymatic activities and resistance to oxidative stress in primary and subcultured rat astroglial cells

A. Vanella, R. Avola, D. F. Condorelli, A. Campisi, A. Costa, A. M Giuffrida Stella, J. R. Perez-Polo

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The survival of neural tissues depends in part on the balance between the formation of free radicals due to oxidative metabolism and the transformation of the free radicals to non-toxic compounds. Serial subculture of rat glial cells as described here resulted in a decrease of the specific activities of several antioxidant enzymes and a glial specific marker for astrocytes. Thus, there was an increased susceptibility to oxidative stress in cultures by the third passage. These subcultured glial cell cultures may represent a useful model for the study of free radical induced neural damage that may be relevant to CNS trauma and aging.

Original languageEnglish (US)
JournalInternational Journal of Developmental Neuroscience
Volume7
Issue number3
DOIs
StatePublished - 1989
Externally publishedYes

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Keywords

  • Aging
  • Antioxidants
  • Astrocytes
  • Free radicals
  • Glia
  • Tissue culture

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

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