TY - JOUR
T1 - Antisera to selected outer membrane proteins of Vibrio cholerae protect against challenge with homologous and heterologous strains of V. cholerae
AU - Das, Margaret
AU - Chopra, Ashok K.
AU - Cantu, Juan M.
AU - Peterson, Johnny W.
N1 - Funding Information:
This work was supported by a grant (DHHS 2RO1 AI21463) to J.W.P. from the National Institutes of Health, Bethesda, MD. Margaret Das was the recipient of a Predoctoral Fellowship from the James W. McLaughlin Fellowship Fund for a part of this study. We thank Mardelle Susman for editorial assistance.
PY - 1998/12
Y1 - 1998/12
N2 - Each year cholera epidemics occur in various places around the world. Though there is no effective vaccine against cholera, people who recover from an infection usually have prolonged immunity to the disease. Sera from convalescent patients contain antibodies to a number of outer membrane proteins (OMPs) of V. cholerae. We isolated several OMPs (43, 42, 30, and 22 kDa) from V. cholerae V86 El Tor Inaba, sequenced their amino-termini, and generated hyperimmune sera against them in rabbits. Antisera to the 43-, 42-, and 22-kDa OMPs, but not the preimmune sera, significantly reduced V. cholerae-induced fluid secretion seen in rabbit intestinal loops challenged with the homologous strain. In addition, a combination of antisera to the different OMPs reduced the fluid secretion induced by challenge with heterologous V. cholerae Ogawa and O139 strains. These results have significance in the development of vaccines to V. cholerae, as the hyperexpression of these OMP encoding genes in vaccine strains may improve the efficacy of cholera vaccines. Copyright (C) 1998 Federation of European Microbiological Societies.
AB - Each year cholera epidemics occur in various places around the world. Though there is no effective vaccine against cholera, people who recover from an infection usually have prolonged immunity to the disease. Sera from convalescent patients contain antibodies to a number of outer membrane proteins (OMPs) of V. cholerae. We isolated several OMPs (43, 42, 30, and 22 kDa) from V. cholerae V86 El Tor Inaba, sequenced their amino-termini, and generated hyperimmune sera against them in rabbits. Antisera to the 43-, 42-, and 22-kDa OMPs, but not the preimmune sera, significantly reduced V. cholerae-induced fluid secretion seen in rabbit intestinal loops challenged with the homologous strain. In addition, a combination of antisera to the different OMPs reduced the fluid secretion induced by challenge with heterologous V. cholerae Ogawa and O139 strains. These results have significance in the development of vaccines to V. cholerae, as the hyperexpression of these OMP encoding genes in vaccine strains may improve the efficacy of cholera vaccines. Copyright (C) 1998 Federation of European Microbiological Societies.
KW - Cholera vaccine
KW - Immunogenic surface protein
KW - Vibrio cholerae
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U2 - 10.1016/S0928-8244(98)00101-1
DO - 10.1016/S0928-8244(98)00101-1
M3 - Article
C2 - 9879921
AN - SCOPUS:0032405753
SN - 0928-8244
VL - 22
SP - 303
EP - 308
JO - FEMS Immunology and Medical Microbiology
JF - FEMS Immunology and Medical Microbiology
IS - 4
ER -