Antiviral activities of ISG20 in positive-strand RNA virus infections

Zhi Zhou, Nan Wang, Sara E. Woodson, Qingming Dong, Jie Wang, Yuqiong Liang, Rene Rijnbrand, Lai Wei, Joan Nichols, Ju Tao Guo, Michael R. Holbrook, Stanley M. Lemon, Kui Li

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

ISG20 is an interferon-inducible 3-5exonuclease that inhibits replication of several human and animal RNA viruses. However, the specificities of ISG20's antiviral action remain poorly defined. Here we determine the impact of ectopic expression of ISG20 on replication of several positive-strand RNA viruses from distinct viral families. ISG20 inhibited infections by cell culture-derived hepatitis C virus (HCV) and a pestivirus, bovine viral diarrhea virus and a picornavirus, hepatitis A virus. Moreover, ISG20 demonstrated cell-type specific antiviral activity against yellow fever virus, a classical flavivirus. Overexpression of ISG20, however, did not inhibit propagation of severe acute respiratory syndrome coronavirus, a highly-pathogenic human coronavirus in Huh7.5 cells. The antiviral effects of ISG20 were all dependent on its exonuclease activity. The closely related cellular exonucleases, ISG20L1 and ISG20L2, did not inhibit HCV replication. Together, these data may help better understand the antiviral specificity and action of ISG20.

Original languageEnglish
Pages (from-to)175-188
Number of pages14
JournalVirology
Volume409
Issue number2
DOIs
StatePublished - Jan 20 2011

Fingerprint

RNA Virus Infections
Antiviral Agents
Exonucleases
Coronavirus
RNA Viruses
Hepacivirus
Pestivirus
Yellow fever virus
Picornaviridae
Bovine Viral Diarrhea Viruses
Flavivirus
Severe Acute Respiratory Syndrome
Hepatitis A virus
Virus Replication
Interferons
Cell Culture Techniques
Viruses
Infection

Keywords

  • Antiviral
  • Bovine viral diarrhea virus
  • Hepatitis A virus
  • Hepatitis C virus
  • Innate immunity
  • Interferon
  • ISG20
  • ISG20L1
  • ISG20L2
  • Severe acute respiratory syndrome coronavirus
  • Yellow fever virus

ASJC Scopus subject areas

  • Virology

Cite this

Zhou, Z., Wang, N., Woodson, S. E., Dong, Q., Wang, J., Liang, Y., ... Li, K. (2011). Antiviral activities of ISG20 in positive-strand RNA virus infections. Virology, 409(2), 175-188. https://doi.org/10.1016/j.virol.2010.10.008

Antiviral activities of ISG20 in positive-strand RNA virus infections. / Zhou, Zhi; Wang, Nan; Woodson, Sara E.; Dong, Qingming; Wang, Jie; Liang, Yuqiong; Rijnbrand, Rene; Wei, Lai; Nichols, Joan; Guo, Ju Tao; Holbrook, Michael R.; Lemon, Stanley M.; Li, Kui.

In: Virology, Vol. 409, No. 2, 20.01.2011, p. 175-188.

Research output: Contribution to journalArticle

Zhou, Z, Wang, N, Woodson, SE, Dong, Q, Wang, J, Liang, Y, Rijnbrand, R, Wei, L, Nichols, J, Guo, JT, Holbrook, MR, Lemon, SM & Li, K 2011, 'Antiviral activities of ISG20 in positive-strand RNA virus infections', Virology, vol. 409, no. 2, pp. 175-188. https://doi.org/10.1016/j.virol.2010.10.008
Zhou Z, Wang N, Woodson SE, Dong Q, Wang J, Liang Y et al. Antiviral activities of ISG20 in positive-strand RNA virus infections. Virology. 2011 Jan 20;409(2):175-188. https://doi.org/10.1016/j.virol.2010.10.008
Zhou, Zhi ; Wang, Nan ; Woodson, Sara E. ; Dong, Qingming ; Wang, Jie ; Liang, Yuqiong ; Rijnbrand, Rene ; Wei, Lai ; Nichols, Joan ; Guo, Ju Tao ; Holbrook, Michael R. ; Lemon, Stanley M. ; Li, Kui. / Antiviral activities of ISG20 in positive-strand RNA virus infections. In: Virology. 2011 ; Vol. 409, No. 2. pp. 175-188.
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