Antiviral effect of interferon lambda against West Nile virus

Dongling Ma, Dong Jiang, Min Qing, Jessica M. Weidner, Xiaowang Qu, Haitao Guo, Jinhong Chang, Baohua Gu, Pei Yong Shi, Timothy M. Block, Ju Tao Guo

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

Type III interferons (IFN), IFN-λ or IL-28/29, are new members of the IFN super-family. Except for using distinct receptors, type I and type III IFNs share the same major post receptor signaling components to activate the transcription of a similar set of IFN-stimulated genes (ISGs). To examine the antiviral effects of the new type IFNs against West Nile virus (WNV), we compared the antiviral effects of IFN-α and IFN-λ on WNV virus-like particle (VLP) infection and replicon replication in Huh7.5 and Hela cells. The results revealed that (i) both types of IFNs could efficiently prevent the WNV infection, but IFN-α demonstrated a stronger antiviral efficacy; (ii) WNV genome replication in VLP-infected cells and replicon-containing cell lines could only be inhibited by IFN-α, but not IFN-λ; (iii) in agreement with the observed antiviral effects, only IFN-λ-induced activation of JAK-STAT signaling pathway and induction of ISG expression were completely inhibited in WNV replicon-containing cell lines, but IFN-α signal transduction was either unaffected or only partially inhibited in Huh7.5 or Hela cells by the virus. Hence, the differential inhibition of WNV on IFN-α and IFN-λ signal transduction implies that the receptors of the two types of IFNs, but not the common post receptor signaling components, could be selectively targeted either directly by WNV nonstructural proteins or indirectly by the cellular responses induced by the virus infection to inhibit the signal transduction of the cytokines.

Original languageEnglish (US)
Pages (from-to)53-60
Number of pages8
JournalAntiviral research
Volume83
Issue number1
DOIs
StatePublished - Jul 1 2009
Externally publishedYes

Keywords

  • Antiviral
  • Interferon
  • West Nile virus

ASJC Scopus subject areas

  • Pharmacology
  • Virology

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  • Cite this

    Ma, D., Jiang, D., Qing, M., Weidner, J. M., Qu, X., Guo, H., Chang, J., Gu, B., Shi, P. Y., Block, T. M., & Guo, J. T. (2009). Antiviral effect of interferon lambda against West Nile virus. Antiviral research, 83(1), 53-60. https://doi.org/10.1016/j.antiviral.2009.03.006