Apelin, a New Enteric Peptide

Localization in the Gastrointestinal Tract, Ontogeny, and Stimulation of Gastric Cell Proliferation and of Cholecystokinin Secretion

Guiyun Wang, Younes Anini, Wei Wei, Xiang Qi, Anne Marie O'Carroll, Tohru Mochizuki, Hui Quin Wang, Mark Hellmich, Ella Englander, George H. Greeley

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

Apelin is a recently discovered peptide that is the endogenous ligand for the APJ receptor. The aim of this study was to characterize apelin expression (mRNA levels) in the rat gastrointestinal tract and pancreas, to localize distribution of apelin peptide-containing cells in the stomach by immunohistochemistry, and to characterize the ontogeny of gastric apelin expression and peptide and the influence of apelin on gastric cell proliferation in vitro. Additionally, the effect of apelin on cholecystokinin (CCK) secretion and the involvement of MAPK, protein kinase C, and changes in intracellular Ca2+ in apelin-induced CCK secretion in vitro were examined. Northern analysis showed a maximal apelin expression in the stomach with a lower expression level in the intestine. Apelin expression was not detected in the pancreas. Immunohistochemistry revealed abundant apelin-positive cells in the glandular epithelium of the stomach. The ontogeny study showed a higher apelin expression in the fetal and postnatal rat stomachs when compared with the adult stomach. In contrast to apelin expression, apelin peptide was not detected in the rat stomach until 20 d of age and then increased progressively with age. Apelin was shown to stimulate gastric cell proliferation in vitro. Apelin also stimulated CCK secretion from a murine enteroendocrine cell line (STC-1); apelin-stimulated CCK secretion is mediated through MAPK but not by intracellular Ca2+ signaling. Together, these data indicate that apelin is an important new stomach peptide with a potential physiological role in the gastrointestinal tract.

Original languageEnglish (US)
Pages (from-to)1342-1348
Number of pages7
JournalEndocrinology
Volume145
Issue number3
DOIs
StatePublished - Mar 2004

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Cholecystokinin
Gastrointestinal Tract
Stomach
Cell Proliferation
Peptides
Pancreas
Immunohistochemistry
Enteroendocrine Cells
Mitogen-Activated Protein Kinase Kinases
Protein Kinase C
Intestines
Epithelium
Ligands
Cell Line
Messenger RNA

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Apelin, a New Enteric Peptide : Localization in the Gastrointestinal Tract, Ontogeny, and Stimulation of Gastric Cell Proliferation and of Cholecystokinin Secretion. / Wang, Guiyun; Anini, Younes; Wei, Wei; Qi, Xiang; O'Carroll, Anne Marie; Mochizuki, Tohru; Wang, Hui Quin; Hellmich, Mark; Englander, Ella; Greeley, George H.

In: Endocrinology, Vol. 145, No. 3, 03.2004, p. 1342-1348.

Research output: Contribution to journalArticle

Wang, Guiyun ; Anini, Younes ; Wei, Wei ; Qi, Xiang ; O'Carroll, Anne Marie ; Mochizuki, Tohru ; Wang, Hui Quin ; Hellmich, Mark ; Englander, Ella ; Greeley, George H. / Apelin, a New Enteric Peptide : Localization in the Gastrointestinal Tract, Ontogeny, and Stimulation of Gastric Cell Proliferation and of Cholecystokinin Secretion. In: Endocrinology. 2004 ; Vol. 145, No. 3. pp. 1342-1348.
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