APE/Ref-1 responses to ischemia in rat brain

Michael Edwards, Thomas A. Kent, Harriet Charmaine Rea, Jinqua Wei, Mike Quast, Tadahide Izumi, Sankar Mitra, J. Regino Perez-Polo

    Research output: Contribution to journalArticle

    35 Citations (Scopus)

    Abstract

    Cerebral ischemia and the aftermath of reperfusion form a hypoxic/hyperoxic sequence of events that can trigger oxidative stress response cascades in neurons of the central nervous system. After transient ischemia there is an increase in intracellular Ca2+ release, extracellular glutamate, reactive oxygen species (ROS) and nitric oxide, genotoxic events that stimulate DNA repair. Increased oxidative stress and interrupted blood flow in ischemia, like DNA repair, also deplete cellular ATP and commit neurons to apoptosis. We report that levels of the DNA repair enzyme apurinic/apyrimidinic endonuclease (APE/Ref-1) decreased significantly in the hippocampus but not other brain areas after 6 h of reperfusion following an induced ischemic insult. This specific inhibition of APE/Ref-1 expression may affect the extent of apoptosis after ischemia.

    Original languageEnglish (US)
    Pages (from-to)4015-4018
    Number of pages4
    JournalNeuroReport
    Volume9
    Issue number18
    StatePublished - Dec 21 1998

    Fingerprint

    Ischemia
    DNA Repair
    Reperfusion
    Brain
    Oxidative Stress
    DNA-(Apurinic or Apyrimidinic Site) Lyase
    Apoptosis
    DNA Repair Enzymes
    Neurons
    Brain Ischemia
    Glutamic Acid
    Reactive Oxygen Species
    Hippocampus
    Nitric Oxide
    Central Nervous System
    Adenosine Triphosphate

    Keywords

    • APE/Ref-1
    • DNA repair
    • Hypoxia
    • Ischemia
    • Reperfusion
    • Stroke

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Edwards, M., Kent, T. A., Rea, H. C., Wei, J., Quast, M., Izumi, T., ... Perez-Polo, J. R. (1998). APE/Ref-1 responses to ischemia in rat brain. NeuroReport, 9(18), 4015-4018.

    APE/Ref-1 responses to ischemia in rat brain. / Edwards, Michael; Kent, Thomas A.; Rea, Harriet Charmaine; Wei, Jinqua; Quast, Mike; Izumi, Tadahide; Mitra, Sankar; Perez-Polo, J. Regino.

    In: NeuroReport, Vol. 9, No. 18, 21.12.1998, p. 4015-4018.

    Research output: Contribution to journalArticle

    Edwards, M, Kent, TA, Rea, HC, Wei, J, Quast, M, Izumi, T, Mitra, S & Perez-Polo, JR 1998, 'APE/Ref-1 responses to ischemia in rat brain', NeuroReport, vol. 9, no. 18, pp. 4015-4018.
    Edwards M, Kent TA, Rea HC, Wei J, Quast M, Izumi T et al. APE/Ref-1 responses to ischemia in rat brain. NeuroReport. 1998 Dec 21;9(18):4015-4018.
    Edwards, Michael ; Kent, Thomas A. ; Rea, Harriet Charmaine ; Wei, Jinqua ; Quast, Mike ; Izumi, Tadahide ; Mitra, Sankar ; Perez-Polo, J. Regino. / APE/Ref-1 responses to ischemia in rat brain. In: NeuroReport. 1998 ; Vol. 9, No. 18. pp. 4015-4018.
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