APE/Ref-1 responses to oxidative stress in aged rats

Michael Edwards, David K. Rassin, Tadahide Izumi, Sankar Mitra, J. Regino Perez-Polo

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Chronic oxidative stress has been hypothesized to be a major contributor to the aging process. The continued exposure to reactive oxygen species (ROS) generated by oxidative metabolism or environmental sources can damage critical cellular structures and be responsible for some age-related pathology. The exposure of rodents to 100% oxygen, isobaric hyperoxia, increases ambient ROS levels and significantly increases apoptosis in brain. The deleterious effects of ROS also include increased lipid peroxidation, protein oxidation, and DNA damage. Although differences in the relative amounts of oxidative stress in young and old brains have been observed, the mechanisms responsible for impaired aging-associated DNA repair processes have not been characterized. We measured DNA levels of the DNA repair enzyme apurinic/apyrimidinic endonuclease (APE/Ref-1) protein by Western blot analysis in the brains of young (3-month) and old (30-month) male rats exposed to isobaric hyperoxia. Given that APE/Ref-1 is the rate-limiting enzyme in the repair pathway of apurinic/apyrimidinic sites generated in DNA by oxidative damage, we assumed that APE/Ref-1 protein levels were a good reflection of ongoing DNA base excision repair. Isobaric hyperoxia stimulated APE/Ref-1 expression in the hippocampus and basal forebrain of young rats experiencing 100% oxygen for 6 hr, while aged rats showed no significant changes in APE/Ref-1 protein levels in all brain areas at any time tested (0- 48 hr) after hyperoxia. Differences in the stress-induced levels of expression of DNA repair enzymes may contribute to apoptotic increases and pathology associated with the aging process.

Original languageEnglish (US)
Pages (from-to)635-638
Number of pages4
JournalJournal of Neuroscience Research
Volume54
Issue number5
DOIs
StatePublished - Dec 1 1998

Fingerprint

Hyperoxia
Oxidative Stress
DNA Repair Enzymes
Reactive Oxygen Species
Brain
DNA Repair
DNA Damage
Proteins
DNA-(Apurinic or Apyrimidinic Site) Lyase
Pathology
Oxygen
DNA
Cellular Structures
Lipid Peroxidation
Rodentia
Hippocampus
Western Blotting
Apoptosis
Enzymes

Keywords

  • APE/Ref-1, DNA repair
  • Hyperoxia
  • Hypoxia
  • Reperfusion

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

APE/Ref-1 responses to oxidative stress in aged rats. / Edwards, Michael; Rassin, David K.; Izumi, Tadahide; Mitra, Sankar; Perez-Polo, J. Regino.

In: Journal of Neuroscience Research, Vol. 54, No. 5, 01.12.1998, p. 635-638.

Research output: Contribution to journalArticle

Edwards, Michael ; Rassin, David K. ; Izumi, Tadahide ; Mitra, Sankar ; Perez-Polo, J. Regino. / APE/Ref-1 responses to oxidative stress in aged rats. In: Journal of Neuroscience Research. 1998 ; Vol. 54, No. 5. pp. 635-638.
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