Apoptosis occurs in a new model of thermal brain injury

Jeff W. Chen, Julian Lin, N. Madamanchi, Todd T. Trier, Gerald Campbell

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Apoptosis has been implicated recently as a prominent response of the brain to a variety of insults, such as ischemia and trauma. In this study, we demonstrate that apoptosis is a prominent part of the brain's response to a thermal insult. To examine the brain's response to a thermal insult, a new model of thermal brain injury in the laboratory rat was developed. Water heated to 60°C was passed over an area of thinned calvarium for 1 min. This resulted in an actual brain temperature of 47-48°C. A uniform area of 2,3,5-triphenyl-tetrazolium chloride pallor was demonstrated and pyknotic neurons were seen in the area of injury by hematoxylin-eosin staining. Apoptosis was demonstrated by the characteristic DNA fragmentation seen by agarose gel electrophoresis, ApopTag in situ staining and electron microscopy. The findings of apoptosis were localized to the area of thermal injury and were time dependent, starting 6 h after the insult and peaking approximately 18 h after the insult. This represents one of the first demonstrations that apoptosis occurs in the brain in response to a thermal injury. Copyright (C) 2000 National Science Council.

Original languageEnglish (US)
Pages (from-to)459-465
Number of pages7
JournalJournal of Biomedical Science
Volume7
Issue number6
StatePublished - 2000

Fingerprint

Brain Injuries
Brain
Hot Temperature
Apoptosis
Wounds and Injuries
Staining and Labeling
Pallor
Agar Gel Electrophoresis
DNA Fragmentation
Hematoxylin
Eosine Yellowish-(YS)
Skull
Chlorides
Electrophoresis
Electron Microscopy
Ischemia
Sepharose
Electron microscopy
Neurons
Rats

Keywords

  • Apoptosis
  • Thermal brain injury

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Chen, J. W., Lin, J., Madamanchi, N., Trier, T. T., & Campbell, G. (2000). Apoptosis occurs in a new model of thermal brain injury. Journal of Biomedical Science, 7(6), 459-465.

Apoptosis occurs in a new model of thermal brain injury. / Chen, Jeff W.; Lin, Julian; Madamanchi, N.; Trier, Todd T.; Campbell, Gerald.

In: Journal of Biomedical Science, Vol. 7, No. 6, 2000, p. 459-465.

Research output: Contribution to journalArticle

Chen, JW, Lin, J, Madamanchi, N, Trier, TT & Campbell, G 2000, 'Apoptosis occurs in a new model of thermal brain injury', Journal of Biomedical Science, vol. 7, no. 6, pp. 459-465.
Chen, Jeff W. ; Lin, Julian ; Madamanchi, N. ; Trier, Todd T. ; Campbell, Gerald. / Apoptosis occurs in a new model of thermal brain injury. In: Journal of Biomedical Science. 2000 ; Vol. 7, No. 6. pp. 459-465.
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