Apparatus and method for interfacing capillary electrophoresis and chemiluminescence nitrogen detection for the analysis of nitrogen-containing compounds

A. A. Mohammad, E. Fujinari, Anthony Okorodudu, J. R. Petersen

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have developed an interface that allows the specific detection of nitrogen-containing compounds by using a chemiluminescence nitrogen detector. The feasibility of using this interface was demonstrated by separating and detecting two nitrogen-containing compounds, p-aminosalicylic acid and l- phenylalanine. Although baseline separation was achieved, the theoretical plates were lower when compared to UV detection (25 000 vs. ~85 000). A sensitivity of 75 ng (~500 pmol) per injection was achieved with this system which is adequate for pharmaceutical and biotech applications.

Original languageEnglish (US)
Pages (from-to)121-125
Number of pages5
JournalJournal of Chromatography A
Volume868
Issue number1
DOIs
StatePublished - Jan 28 2000

Fingerprint

Nitrogen Compounds
Capillary electrophoresis
Chemiluminescence
Capillary Electrophoresis
Luminescence
Nitrogen
Aminosalicylic Acid
Phenylalanine
Injections
Pharmaceutical Preparations
Detectors

Keywords

  • Aminosalicylic acid
  • CE-chemiluminescence nitrogen detection
  • Detection, electrophoresis
  • Instrumentation
  • Interfaces
  • Phenylalanine

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Apparatus and method for interfacing capillary electrophoresis and chemiluminescence nitrogen detection for the analysis of nitrogen-containing compounds. / Mohammad, A. A.; Fujinari, E.; Okorodudu, Anthony; Petersen, J. R.

In: Journal of Chromatography A, Vol. 868, No. 1, 28.01.2000, p. 121-125.

Research output: Contribution to journalArticle

@article{fb3de697928f445aaedd0c79e6c3b482,
title = "Apparatus and method for interfacing capillary electrophoresis and chemiluminescence nitrogen detection for the analysis of nitrogen-containing compounds",
abstract = "We have developed an interface that allows the specific detection of nitrogen-containing compounds by using a chemiluminescence nitrogen detector. The feasibility of using this interface was demonstrated by separating and detecting two nitrogen-containing compounds, p-aminosalicylic acid and l- phenylalanine. Although baseline separation was achieved, the theoretical plates were lower when compared to UV detection (25 000 vs. ~85 000). A sensitivity of 75 ng (~500 pmol) per injection was achieved with this system which is adequate for pharmaceutical and biotech applications.",
keywords = "Aminosalicylic acid, CE-chemiluminescence nitrogen detection, Detection, electrophoresis, Instrumentation, Interfaces, Phenylalanine",
author = "Mohammad, {A. A.} and E. Fujinari and Anthony Okorodudu and Petersen, {J. R.}",
year = "2000",
month = "1",
day = "28",
doi = "10.1016/S0021-9673(99)01155-3",
language = "English (US)",
volume = "868",
pages = "121--125",
journal = "Journal of Chromatography",
issn = "0021-9673",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Apparatus and method for interfacing capillary electrophoresis and chemiluminescence nitrogen detection for the analysis of nitrogen-containing compounds

AU - Mohammad, A. A.

AU - Fujinari, E.

AU - Okorodudu, Anthony

AU - Petersen, J. R.

PY - 2000/1/28

Y1 - 2000/1/28

N2 - We have developed an interface that allows the specific detection of nitrogen-containing compounds by using a chemiluminescence nitrogen detector. The feasibility of using this interface was demonstrated by separating and detecting two nitrogen-containing compounds, p-aminosalicylic acid and l- phenylalanine. Although baseline separation was achieved, the theoretical plates were lower when compared to UV detection (25 000 vs. ~85 000). A sensitivity of 75 ng (~500 pmol) per injection was achieved with this system which is adequate for pharmaceutical and biotech applications.

AB - We have developed an interface that allows the specific detection of nitrogen-containing compounds by using a chemiluminescence nitrogen detector. The feasibility of using this interface was demonstrated by separating and detecting two nitrogen-containing compounds, p-aminosalicylic acid and l- phenylalanine. Although baseline separation was achieved, the theoretical plates were lower when compared to UV detection (25 000 vs. ~85 000). A sensitivity of 75 ng (~500 pmol) per injection was achieved with this system which is adequate for pharmaceutical and biotech applications.

KW - Aminosalicylic acid

KW - CE-chemiluminescence nitrogen detection

KW - Detection, electrophoresis

KW - Instrumentation

KW - Interfaces

KW - Phenylalanine

UR - http://www.scopus.com/inward/record.url?scp=0033979076&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033979076&partnerID=8YFLogxK

U2 - 10.1016/S0021-9673(99)01155-3

DO - 10.1016/S0021-9673(99)01155-3

M3 - Article

VL - 868

SP - 121

EP - 125

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0021-9673

IS - 1

ER -