Application of Adcon-P or Seprafilm in consecutive laparotomies using a murine model

Mustafa Oncel, Feza H. Remzi, Anthony J. Senagore, Jason T. Connor, Victor W. Fazio

    Research output: Contribution to journalArticle

    26 Scopus citations

    Abstract

    Background: Intraabdominal administration of antiadhesive products may reduce postoperative adhesions. We compared two barriers, Adcon-P (Gliatech, Cleveland, Ohio) and Seprafilm (Genzyme, Cambridge, Massachusetts), in a relaparotomy murine model to evaluate whether using them during the first and second laparotomy reduces adhesion formation better than using them only during the relaparotomy. Methods: One-hundred and fifty mice underwent cecal abrasion, followed by complete adhesiolysis and cecal abrasion 21 days later. All were sacrified 21 days after the second operation. The mice (30 to a group) received Adcon-P in both surgeries (AA), Seprafilm in both surgeries (SS), Adcon-P in the second surgery (CA), Seprafilm in the second surgery (CS), or neither barrier (CC). Difficulty of adhesiolysis was blindly assessed with a 6-point scoring system and sites of adhesions were determined with a yes/no questionnaire. Results: The AA group had lower adhesiolysis score than SS, CS, and CC groups, and had fewer adhesions over cecum and between cecum and intra-peritoneal fat than all other groups. The numbers of the animals had adhesions between the cecum and abdominal wall were similar between the treatment groups. No difference was observed between SS and CS groups in any analysis. Conclusions: Using Adcon-P starting with the first laparotomy more effectively reduce adhesion formation and difficulty of adhesiolysis. Using Seprafilm only in the relaparotomy is as effective as using it in both laparotomies. Adcon-P is more effective in preventing adhesions to different sites than Seprafilm except to the abdominal wall under the midline incision.

    Original languageEnglish (US)
    Pages (from-to)304-308
    Number of pages5
    JournalAmerican Journal of Surgery
    Volume187
    Issue number2
    DOIs
    StatePublished - Feb 2004

    Keywords

    • Adcon-P
    • Adhesiolysis
    • Postoperative adhesions
    • Seprafilm

    ASJC Scopus subject areas

    • Surgery

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