Application of Adcon-P or Seprafilm in consecutive laparotomies using a murine model

Mustafa Oncel, Feza H. Remzi, Anthony J. Senagore, Jason T. Connor, Victor W. Fazio

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Background: Intraabdominal administration of antiadhesive products may reduce postoperative adhesions. We compared two barriers, Adcon-P (Gliatech, Cleveland, Ohio) and Seprafilm (Genzyme, Cambridge, Massachusetts), in a relaparotomy murine model to evaluate whether using them during the first and second laparotomy reduces adhesion formation better than using them only during the relaparotomy. Methods: One-hundred and fifty mice underwent cecal abrasion, followed by complete adhesiolysis and cecal abrasion 21 days later. All were sacrified 21 days after the second operation. The mice (30 to a group) received Adcon-P in both surgeries (AA), Seprafilm in both surgeries (SS), Adcon-P in the second surgery (CA), Seprafilm in the second surgery (CS), or neither barrier (CC). Difficulty of adhesiolysis was blindly assessed with a 6-point scoring system and sites of adhesions were determined with a yes/no questionnaire. Results: The AA group had lower adhesiolysis score than SS, CS, and CC groups, and had fewer adhesions over cecum and between cecum and intra-peritoneal fat than all other groups. The numbers of the animals had adhesions between the cecum and abdominal wall were similar between the treatment groups. No difference was observed between SS and CS groups in any analysis. Conclusions: Using Adcon-P starting with the first laparotomy more effectively reduce adhesion formation and difficulty of adhesiolysis. Using Seprafilm only in the relaparotomy is as effective as using it in both laparotomies. Adcon-P is more effective in preventing adhesions to different sites than Seprafilm except to the abdominal wall under the midline incision.

Original languageEnglish (US)
Pages (from-to)304-308
Number of pages5
JournalAmerican Journal of Surgery
Volume187
Issue number2
DOIs
StatePublished - Feb 2004
Externally publishedYes

Fingerprint

Laparotomy
Cecum
Abdominal Wall
Adcon-P
Seprafilm
Fats

Keywords

  • Adcon-P
  • Adhesiolysis
  • Postoperative adhesions
  • Seprafilm

ASJC Scopus subject areas

  • Surgery

Cite this

Application of Adcon-P or Seprafilm in consecutive laparotomies using a murine model. / Oncel, Mustafa; Remzi, Feza H.; Senagore, Anthony J.; Connor, Jason T.; Fazio, Victor W.

In: American Journal of Surgery, Vol. 187, No. 2, 02.2004, p. 304-308.

Research output: Contribution to journalArticle

Oncel, Mustafa ; Remzi, Feza H. ; Senagore, Anthony J. ; Connor, Jason T. ; Fazio, Victor W. / Application of Adcon-P or Seprafilm in consecutive laparotomies using a murine model. In: American Journal of Surgery. 2004 ; Vol. 187, No. 2. pp. 304-308.
@article{9c5b2685cfb44bd893c90ba2ced2198e,
title = "Application of Adcon-P or Seprafilm in consecutive laparotomies using a murine model",
abstract = "Background: Intraabdominal administration of antiadhesive products may reduce postoperative adhesions. We compared two barriers, Adcon-P (Gliatech, Cleveland, Ohio) and Seprafilm (Genzyme, Cambridge, Massachusetts), in a relaparotomy murine model to evaluate whether using them during the first and second laparotomy reduces adhesion formation better than using them only during the relaparotomy. Methods: One-hundred and fifty mice underwent cecal abrasion, followed by complete adhesiolysis and cecal abrasion 21 days later. All were sacrified 21 days after the second operation. The mice (30 to a group) received Adcon-P in both surgeries (AA), Seprafilm in both surgeries (SS), Adcon-P in the second surgery (CA), Seprafilm in the second surgery (CS), or neither barrier (CC). Difficulty of adhesiolysis was blindly assessed with a 6-point scoring system and sites of adhesions were determined with a yes/no questionnaire. Results: The AA group had lower adhesiolysis score than SS, CS, and CC groups, and had fewer adhesions over cecum and between cecum and intra-peritoneal fat than all other groups. The numbers of the animals had adhesions between the cecum and abdominal wall were similar between the treatment groups. No difference was observed between SS and CS groups in any analysis. Conclusions: Using Adcon-P starting with the first laparotomy more effectively reduce adhesion formation and difficulty of adhesiolysis. Using Seprafilm only in the relaparotomy is as effective as using it in both laparotomies. Adcon-P is more effective in preventing adhesions to different sites than Seprafilm except to the abdominal wall under the midline incision.",
keywords = "Adcon-P, Adhesiolysis, Postoperative adhesions, Seprafilm",
author = "Mustafa Oncel and Remzi, {Feza H.} and Senagore, {Anthony J.} and Connor, {Jason T.} and Fazio, {Victor W.}",
year = "2004",
month = "2",
doi = "10.1016/j.amjsurg.2003.11.014",
language = "English (US)",
volume = "187",
pages = "304--308",
journal = "American Journal of Surgery",
issn = "0002-9610",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Application of Adcon-P or Seprafilm in consecutive laparotomies using a murine model

AU - Oncel, Mustafa

AU - Remzi, Feza H.

AU - Senagore, Anthony J.

AU - Connor, Jason T.

AU - Fazio, Victor W.

PY - 2004/2

Y1 - 2004/2

N2 - Background: Intraabdominal administration of antiadhesive products may reduce postoperative adhesions. We compared two barriers, Adcon-P (Gliatech, Cleveland, Ohio) and Seprafilm (Genzyme, Cambridge, Massachusetts), in a relaparotomy murine model to evaluate whether using them during the first and second laparotomy reduces adhesion formation better than using them only during the relaparotomy. Methods: One-hundred and fifty mice underwent cecal abrasion, followed by complete adhesiolysis and cecal abrasion 21 days later. All were sacrified 21 days after the second operation. The mice (30 to a group) received Adcon-P in both surgeries (AA), Seprafilm in both surgeries (SS), Adcon-P in the second surgery (CA), Seprafilm in the second surgery (CS), or neither barrier (CC). Difficulty of adhesiolysis was blindly assessed with a 6-point scoring system and sites of adhesions were determined with a yes/no questionnaire. Results: The AA group had lower adhesiolysis score than SS, CS, and CC groups, and had fewer adhesions over cecum and between cecum and intra-peritoneal fat than all other groups. The numbers of the animals had adhesions between the cecum and abdominal wall were similar between the treatment groups. No difference was observed between SS and CS groups in any analysis. Conclusions: Using Adcon-P starting with the first laparotomy more effectively reduce adhesion formation and difficulty of adhesiolysis. Using Seprafilm only in the relaparotomy is as effective as using it in both laparotomies. Adcon-P is more effective in preventing adhesions to different sites than Seprafilm except to the abdominal wall under the midline incision.

AB - Background: Intraabdominal administration of antiadhesive products may reduce postoperative adhesions. We compared two barriers, Adcon-P (Gliatech, Cleveland, Ohio) and Seprafilm (Genzyme, Cambridge, Massachusetts), in a relaparotomy murine model to evaluate whether using them during the first and second laparotomy reduces adhesion formation better than using them only during the relaparotomy. Methods: One-hundred and fifty mice underwent cecal abrasion, followed by complete adhesiolysis and cecal abrasion 21 days later. All were sacrified 21 days after the second operation. The mice (30 to a group) received Adcon-P in both surgeries (AA), Seprafilm in both surgeries (SS), Adcon-P in the second surgery (CA), Seprafilm in the second surgery (CS), or neither barrier (CC). Difficulty of adhesiolysis was blindly assessed with a 6-point scoring system and sites of adhesions were determined with a yes/no questionnaire. Results: The AA group had lower adhesiolysis score than SS, CS, and CC groups, and had fewer adhesions over cecum and between cecum and intra-peritoneal fat than all other groups. The numbers of the animals had adhesions between the cecum and abdominal wall were similar between the treatment groups. No difference was observed between SS and CS groups in any analysis. Conclusions: Using Adcon-P starting with the first laparotomy more effectively reduce adhesion formation and difficulty of adhesiolysis. Using Seprafilm only in the relaparotomy is as effective as using it in both laparotomies. Adcon-P is more effective in preventing adhesions to different sites than Seprafilm except to the abdominal wall under the midline incision.

KW - Adcon-P

KW - Adhesiolysis

KW - Postoperative adhesions

KW - Seprafilm

UR - http://www.scopus.com/inward/record.url?scp=0842266062&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0842266062&partnerID=8YFLogxK

U2 - 10.1016/j.amjsurg.2003.11.014

DO - 10.1016/j.amjsurg.2003.11.014

M3 - Article

VL - 187

SP - 304

EP - 308

JO - American Journal of Surgery

JF - American Journal of Surgery

SN - 0002-9610

IS - 2

ER -