@inbook{98063841f8f547868e41d412c57fdfd5,
title = "Application of droplet digital PCR to validate rift valley fever vaccines",
abstract = "Droplet Digital{\texttrademark} polymerase chain reaction (ddPCR{\texttrademark}) is a promising technique that quantitates the absolute concentration of nucleic acids in a given sample. This technique utilizes water-in-oil emulsion technology, a system developed by Bio-Rad Laboratories that partitions a single sample into thousands of nanoliter-sized droplets and counts nucleic acid molecules encapsulated in each individual particle as one PCR reaction. This chapter discusses the applications and methodologies of ddPCR for development of Rift Valley fever (RVF) vaccine, using an example that measures RNA copy numbers of a live-attenuated MP-12 vaccine from virus stocks, infected cells, or animal blood. We also discuss how ddPCR detects a reversion mutant of MP-12 from virus stocks accurately. The use of ddPCR improves the quality control of live-attenuated vaccines in the seed lot systems.",
keywords = "Copy number validation, Droplet digital PCR, MP-12 vaccine, Rift valley fever, Variant RNA detection",
author = "Ly, {Hoai J.} and Nandadeva Lokugamage and Tetsuro Ikegami",
note = "Funding Information: We thank Dr. Raymond Miller (Bio-Rad) for initial setup of ddPCR experiment. This study was supported by NIH grant R01 AI087643, and the funding from the Sealy Center for Vaccine Development at The University of Texas Medical Branch at Galveston. Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
month = apr,
day = "1",
doi = "10.1007/978-1-4939-3387-7_10",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "207--220",
booktitle = "Methods in Molecular Biology",
}