Applications of piezoelectric fluid jetting devices to neuroscience research

Gary L. Bernardini, B. Allan Rampy, Gailyn A. Howell, Donald J. Hayes, Christopher J. Frederickson

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Piezoelectric pumps or "jets" are used in industry for precise dispensing of small volumes of fluids. In the present work we have tested the feasibility of using these piezoelectric devices for dispensing fluids in neurobiological research. In one experiment, 70 picoliter (pl) droplets of histochemical reagent were jetted onto discrete targets of frozen tissue sections, and qualitative and quantitative histochemical studies were done on the small (170 μm diameter) circle of tissue wet by the droplets. In the second experiment, 70 pl droplets of neuroactive drugs were jetted onto brain tissue slices while recording single neurons extracellularly in vitro, and the effects of the drugs were found to vary systematically as a function of the number of drops and the distance between drop application and the recorded neuron. The results indicate that piezoelectric jets could have wide application for dispensing fluids in neurobiological research.

Original languageEnglish (US)
Pages (from-to)81-88
Number of pages8
JournalJournal of Neuroscience Methods
Volume38
Issue number1
DOIs
StatePublished - Jun 1991

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Keywords

  • Brain slices
  • Drug application
  • Fluid dispensing
  • Histochemistry
  • Neurophysiology
  • Pumps

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Bernardini, G. L., Rampy, B. A., Howell, G. A., Hayes, D. J., & Frederickson, C. J. (1991). Applications of piezoelectric fluid jetting devices to neuroscience research. Journal of Neuroscience Methods, 38(1), 81-88. https://doi.org/10.1016/0165-0270(91)90157-U