Aptamers containing thymidine 3'-O-phosphorodithioates

Synthesis and binding to nuclear factor-κB

Xianbin Yang, Susan Fennewald, Bruce A. Luxon, Judith Aronson, Norbert K. Herzog, David G. Gorenstein

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Aptamers targeting NF-κB containing thymidine 3'-O-phosphorodithioates in selected positions of an oligonucleotide duplex were synthesized. Binding affinities to NF-κB varied with the number and positions of the dithioate backbone substitutions. One of the aptamers showed specific binding to a single NF-κB dimer in cell culture extracts.

Original languageEnglish (US)
Pages (from-to)3357-3362
Number of pages6
JournalBioorganic and Medicinal Chemistry Letters
Volume9
Issue number23
StatePublished - Dec 6 1999

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Cell Extracts
Cell culture
Oligonucleotides
Dimers
Thymidine
Substitution reactions
Cell Culture Techniques
phosphorodithioic acid

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

Cite this

Yang, X., Fennewald, S., Luxon, B. A., Aronson, J., Herzog, N. K., & Gorenstein, D. G. (1999). Aptamers containing thymidine 3'-O-phosphorodithioates: Synthesis and binding to nuclear factor-κB. Bioorganic and Medicinal Chemistry Letters, 9(23), 3357-3362.

Aptamers containing thymidine 3'-O-phosphorodithioates : Synthesis and binding to nuclear factor-κB. / Yang, Xianbin; Fennewald, Susan; Luxon, Bruce A.; Aronson, Judith; Herzog, Norbert K.; Gorenstein, David G.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 9, No. 23, 06.12.1999, p. 3357-3362.

Research output: Contribution to journalArticle

Yang, X, Fennewald, S, Luxon, BA, Aronson, J, Herzog, NK & Gorenstein, DG 1999, 'Aptamers containing thymidine 3'-O-phosphorodithioates: Synthesis and binding to nuclear factor-κB', Bioorganic and Medicinal Chemistry Letters, vol. 9, no. 23, pp. 3357-3362.
Yang, Xianbin ; Fennewald, Susan ; Luxon, Bruce A. ; Aronson, Judith ; Herzog, Norbert K. ; Gorenstein, David G. / Aptamers containing thymidine 3'-O-phosphorodithioates : Synthesis and binding to nuclear factor-κB. In: Bioorganic and Medicinal Chemistry Letters. 1999 ; Vol. 9, No. 23. pp. 3357-3362.
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