Apurinic endonuclease activity of yeast Apn2 protein

Ildiko Unk, Lajos Haracska, Robert E. Johnson, Satya Prakash, Louise Prakash

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Abasic (apurinic/apyrimidinic; AP) sites are generated in vivo through spontaneous base loss and by enzymatic removal of bases damaged by alkylating agents and reactive oxygen species. In Saccharomyces cerevisiae, the APN1 and APN2 genes function in alternate pathways of AP site removal. Apn2-like proteins have been identified in other eukaryotes including humans, and these proteins form a distinct subfamily within the exonuclease III (ExoIII)/Ape1/Apn2 family of proteins. Apn2 and other members of this subfamily contain a carboxyl-terminal extension not present in the ExoIII/Ape1-like proteins. Here, we purify the Apn2 protein from yeast and show that it is a class II AP endonuclease. Deletion of the carboxyl terminus does not affect the AP endonuclease activity of the protein, but this protein is defective in the removal of AP sites in vivo. The carboxyl terminus may enable Apn2 to complex with other proteins, and such a multiprotein assembly may be necessary for the efficient recognition and cleavage of AP sites in vivo.

Original languageEnglish (US)
Pages (from-to)22427-22434
Number of pages8
JournalJournal of Biological Chemistry
Volume275
Issue number29
DOIs
StatePublished - Jul 21 2000

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DNA-(Apurinic or Apyrimidinic Site) Lyase
Fungal Proteins
Proteins
Alkylating Agents
Eukaryota
Yeast
Saccharomyces cerevisiae
Reactive Oxygen Species
Genes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Apurinic endonuclease activity of yeast Apn2 protein. / Unk, Ildiko; Haracska, Lajos; Johnson, Robert E.; Prakash, Satya; Prakash, Louise.

In: Journal of Biological Chemistry, Vol. 275, No. 29, 21.07.2000, p. 22427-22434.

Research output: Contribution to journalArticle

Unk, Ildiko ; Haracska, Lajos ; Johnson, Robert E. ; Prakash, Satya ; Prakash, Louise. / Apurinic endonuclease activity of yeast Apn2 protein. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 29. pp. 22427-22434.
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