Arachidonic acid inhibits 5-lipoxygenase in human T cells

Durgaprasadarao Atluru; Elias A. Lianos; James S. Goodwin

doi:10.1016/0006-291X(86)90045-8

Arachidonic acid inhibits 5-lipoxygenase in human T cells

Durgaprasadarao Atluru, Elias A. Lianos, James S. Goodwin

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The data on whether T cells produce leukotrienes or other 5-lipoxygenase metabolites of arachidonic acid is conflicting. We report that exogenous arachidonic acid added to phytohemagglutin-stimulated human T cells profoundly inhibits leukotriene B4 production, with 90% inhibition caused by 10^-6 M arachidonic acid. The 12-and 15-lipoxygenase pathways were also inhibited by arachidonic acid. Recent reports that human T cells produce no 5-lipoxygenase metabolites of arachidonic acid might be explained by the fact that the studies used ≥ 10^-5M arachidonic acid in the incubation media.

Original language	English (US)
Pages (from-to)	670-676
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	135
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(86)90045-8
State	Published - Mar 13 1986
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(86)90045-8

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title = "Arachidonic acid inhibits 5-lipoxygenase in human T cells",

abstract = "The data on whether T cells produce leukotrienes or other 5-lipoxygenase metabolites of arachidonic acid is conflicting. We report that exogenous arachidonic acid added to phytohemagglutin-stimulated human T cells profoundly inhibits leukotriene B4 production, with 90% inhibition caused by 10-6 M arachidonic acid. The 12-and 15-lipoxygenase pathways were also inhibited by arachidonic acid. Recent reports that human T cells produce no 5-lipoxygenase metabolites of arachidonic acid might be explained by the fact that the studies used ≥ 10-5M arachidonic acid in the incubation media.",

author = "Durgaprasadarao Atluru and Lianos, {Elias A.} and Goodwin, {James S.}",

note = "Funding Information: This work was supported the U.S. Public Health Heart Association.",

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T1 - Arachidonic acid inhibits 5-lipoxygenase in human T cells

AU - Atluru, Durgaprasadarao

AU - Lianos, Elias A.

AU - Goodwin, James S.

N1 - Funding Information: This work was supported the U.S. Public Health Heart Association.

PY - 1986/3/13

Y1 - 1986/3/13

N2 - The data on whether T cells produce leukotrienes or other 5-lipoxygenase metabolites of arachidonic acid is conflicting. We report that exogenous arachidonic acid added to phytohemagglutin-stimulated human T cells profoundly inhibits leukotriene B4 production, with 90% inhibition caused by 10-6 M arachidonic acid. The 12-and 15-lipoxygenase pathways were also inhibited by arachidonic acid. Recent reports that human T cells produce no 5-lipoxygenase metabolites of arachidonic acid might be explained by the fact that the studies used ≥ 10-5M arachidonic acid in the incubation media.

AB - The data on whether T cells produce leukotrienes or other 5-lipoxygenase metabolites of arachidonic acid is conflicting. We report that exogenous arachidonic acid added to phytohemagglutin-stimulated human T cells profoundly inhibits leukotriene B4 production, with 90% inhibition caused by 10-6 M arachidonic acid. The 12-and 15-lipoxygenase pathways were also inhibited by arachidonic acid. Recent reports that human T cells produce no 5-lipoxygenase metabolites of arachidonic acid might be explained by the fact that the studies used ≥ 10-5M arachidonic acid in the incubation media.

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Arachidonic acid inhibits 5-lipoxygenase in human T cells

Abstract

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