Are Interstitial Cells of Cajal Involved in Mechanical Stress-Induced Gene Expression and Impairment of Smooth Muscle Contractility in Bowel Obstruction?

Chester C. Wu, Youmin Lin, Jerry Gao, John H. Winston, Leo K. Cheng, Xuan Zheng Shi

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Background and Aims:The network of interstitial cells of Cajal (ICC) is altered in obstructive bowel disorders (OBD). However, whether alteration in ICC network is a cause or consequence of OBD remains unknown. This study tested the hypothesis that mechanical dilation in obstruction disrupts the ICC network and that ICC do not mediate mechanotranscription of COX-2 and impairment of smooth muscle contractility in obstruction.Methods:Medical-grade silicon bands were wrapped around the distal colon to induce partial obstruction in wild-type and ICC deficient (W/Wv) mice.Results:In wild-type mice, colon obstruction led to time-dependent alterations of the ICC network in the proximal colon segment. Although unaffected on days 1 and 3, the ICC density decreased markedly and the network was disrupted on day 7 of obstruction. COX-2 expression increased, and circular muscle contractility decreased significantly in the segment proximal to obstruction. In W/Wv control mice, COX-2 mRNA level was 4.0 (±1.1)-fold higher (n=4) and circular muscle contractility was lower than in wild-type control mice. Obstruction further increased COX-2 mRNA level in W/Wv mice to 7.2 (±1.0)-fold vs. W/Wv controls [28.8 (±4.1)-fold vs. wild-type controls] on day 3. Obstruction further suppressed smooth muscle contractility in W/Wv mice. However, daily administration of COX-2 inhibitor NS-398 significantly improved muscle contractility in both W/Wv sham and obstruction mice.Conclusions:Lumen dilation disrupts the ICC network. ICC deficiency has limited effect on stretch-induced expression of COX-2 and suppression of smooth muscle contractility in obstruction. Rather, stretch-induced COX-2 plays a critical role in motility dysfunction in partial colon obstruction.

Original languageEnglish (US)
Article numbere76222
JournalPloS one
Volume8
Issue number9
DOIs
StatePublished - Sep 30 2013

ASJC Scopus subject areas

  • General

Fingerprint

Dive into the research topics of 'Are Interstitial Cells of Cajal Involved in Mechanical Stress-Induced Gene Expression and Impairment of Smooth Muscle Contractility in Bowel Obstruction?'. Together they form a unique fingerprint.

Cite this